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. 1970 Aug;6(2):149–155. doi: 10.1128/jvi.6.2.149-155.1970

Degradation of Escherichia coli B Deoxyribonucleic Acid After Infection with Deoxyribonucleic Acid-Defective Amber Mutants of Bacteriophage T7 1

Paul D Sadowski 1,2, Carol Kerr 1
PMCID: PMC376101  PMID: 4925772

Abstract

The degradation of bacterial deoxyribonucleic acid (DNA) was studied after infection of Escherichia coli B with DNA-negative amber mutants of bacteriophage T7. Degradation occurred in three stages. (i) Release of the DNA from a rapidly sedimenting cellular structure occurred between 5 and 6 min after infection. (ii) The DNA was cleaved endonucleolytically to fragments having a molecular weight of about 2 × 106 between 6 and 10 min after infection. (iii) These fragments of DNA were reduced to acid-soluble products between 7.5 and 15 min after infection. Stage 1 did not occur in the absence of the gene 1 product (ribonucleic acid polymerase sigma factor), stage 2 did not occur in the absence of the gene 3 product (phage T7-induced endonuclease), and stage 3 did not occur in the absence of the gene 6 product.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURGI E., HERSHEY A. D. Sedimentation rate as a measure of molecular weight of DNA. Biophys J. 1963 Jul;3:309–321. doi: 10.1016/s0006-3495(63)86823-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bose S. K., Warren R. J. Bacteriophage-induced inhibition of host functions. II. Evidence for multiple, sequential bacteriophage-induced deoxyribonucleases responsible for degradation of cellular deoxyribonucleic acid. J Virol. 1969 Jun;3(6):549–556. doi: 10.1128/jvi.3.6.549-556.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CRAWFORD L. V. Nucleic acid metabolism in Escherichia coli infected with phage T5. Virology. 1959 Apr;7(4):359–374. doi: 10.1016/0042-6822(59)90065-0. [DOI] [PubMed] [Google Scholar]
  4. Center M. S., Studier F. W., Richardson C. C. The structural gene for a T7 endonuclease essential for phage DNA synthesis. Proc Natl Acad Sci U S A. 1970 Jan;65(1):242–248. doi: 10.1073/pnas.65.1.242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FRASER D., JERREL E. A. The amino acid composition of T3 bacteriophage. J Biol Chem. 1953 Nov;205(1):291–295. [PubMed] [Google Scholar]
  6. HERSHEY A. D., DIXON J., CHASE M. Nucleic acid economy in bacteria infected with bacteriophage T2. I. Purine and pyrimidine composition. J Gen Physiol. 1953 Jul;36(6):777–789. doi: 10.1085/jgp.36.6.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hausmann R., Gomez B. Amber mutants of bacteriophages T3 and T7 defective in phage-directed deoxyribonucleic acid synthesis. J Virol. 1967 Aug;1(4):779–792. doi: 10.1128/jvi.1.4.779-792.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KELLENBERGER E., SECHAUD J., RYTER A. Electron microscopical studies of phage multiplication. IV. The establishment of the DNA pool of vegetative phage and the maturation of phage particles. Virology. 1959 Aug;8:478–498. doi: 10.1016/0042-6822(59)90050-9. [DOI] [PubMed] [Google Scholar]
  9. KOZLOFF L. M. Origin and fate of bacteriophage material. Cold Spring Harb Symp Quant Biol. 1953;18:209–220. doi: 10.1101/sqb.1953.018.01.032. [DOI] [PubMed] [Google Scholar]
  10. Kutter E. M., Wiberg J. S. Degradation of cytosin-containing bacterial and bacteriophage DNA after infection of Escherichia coli B with bacteriophage T4D wild type and with mutants defective in genes 46, 47 and 56. J Mol Biol. 1968 Dec;38(3):395–411. doi: 10.1016/0022-2836(68)90394-x. [DOI] [PubMed] [Google Scholar]
  11. LABAW L. W. The origin of phosphorus in Escherichia coli bacteriophages. J Bacteriol. 1951 Aug;62(2):169–173. doi: 10.1128/jb.62.2.169-173.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LABAW L. W. The origin of phosphorus in the T1, T5, T6, and T7 bacteriophages of Escherichia coli. J Bacteriol. 1953 Oct;66(4):429–436. doi: 10.1128/jb.66.4.429-436.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LURIA S. E., HUMAN M. L. Chromatin staining of bacteria during bacteriophage infection. J Bacteriol. 1950 Apr;59(4):551–560. doi: 10.1128/jb.59.4.551-560.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. STONE A. B., BURTON K. Studies on the deoxyribonucleases of bacteriophage-infected Escherichia coli. Biochem J. 1962 Dec;85:600–606. doi: 10.1042/bj0850600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sadowski P. D., Hurwitz J. Enzymatic breakage of deoxyribonucleic acid. I. Purification and properties of endonuclease II from T4 phage-infected Escherichia coli. J Biol Chem. 1969 Nov 25;244(22):6182–6191. [PubMed] [Google Scholar]
  16. Sadowski P. D., Hurwitz J. Enzymatic breakage of deoxyribonucleic acid. II. Purification and properties of endonuclease IV from T4 phage-infected Escherichia coli. J Biol Chem. 1969 Nov 25;244(22):6192–6198. [PubMed] [Google Scholar]
  17. Studier F. W., Hausmann R. Integration of two sets of T7 mutants. Virology. 1969 Nov;39(3):587–588. doi: 10.1016/0042-6822(69)90106-8. [DOI] [PubMed] [Google Scholar]
  18. Studier F. W., Maizel J. V., Jr T7-directed protein synthesis. Virology. 1969 Nov;39(3):575–586. doi: 10.1016/0042-6822(69)90105-6. [DOI] [PubMed] [Google Scholar]
  19. Studier F. W. The genetics and physiology of bacteriophage T7. Virology. 1969 Nov;39(3):562–574. doi: 10.1016/0042-6822(69)90104-4. [DOI] [PubMed] [Google Scholar]
  20. Summers W. C., Siegel R. B. Control of template specificity of E. coli RNA polymerase by a phage-coded protein. Nature. 1969 Sep 13;223(5211):1111–1113. doi: 10.1038/2231111a0. [DOI] [PubMed] [Google Scholar]
  21. Tremblay G. Y., Daniels M. J., Schaechter M. Isolation of a cell membrane-DNA-nascent RNA complex from bacteria. J Mol Biol. 1969 Feb 28;40(1):65–76. doi: 10.1016/0022-2836(69)90296-4. [DOI] [PubMed] [Google Scholar]
  22. Warren R. J., Bose S. K. Bacteriophage-induced inhibition of host functions. I. Degradation of Escherichia coli deoxyribonucleic acid after T4 infection. J Virol. 1968 Apr;2(4):327–334. doi: 10.1128/jvi.2.4.327-334.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wiberg J. S. Mutants of bacteriophage T4 unable to cause breakdown of host DNA. Proc Natl Acad Sci U S A. 1966 Mar;55(3):614–621. doi: 10.1073/pnas.55.3.614. [DOI] [PMC free article] [PubMed] [Google Scholar]

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