Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1970 Aug;6(2):169–175. doi: 10.1128/jvi.6.2.169-175.1970

Membrane Binding of Input Arbovirus Ribonucleic Acid: Effect of Interferon or Cycloheximide

Robert M Friedman 1,2, T Sreevalsan 1,2
PMCID: PMC376104  PMID: 5533703

Abstract

By 1 hr after infection, 36% of input Semliki Forest virus ribonucleic acid (RNA) which was cell associated was found in a membrane structure. This structure had many similarities to the membrane-associated replication complex (MRC) which had previously been identified in arbovirus infections. Interferon treatment did not affect the association of viral RNA with the MRC structure, but cycloheximide treatment inhibited it.

Full text

PDF
169

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHENG P. Y. Purification, size, and morphology of a mosquitoborne animal virus, Semliki Forest virus. Virology. 1961 May;14:124–131. doi: 10.1016/0042-6822(61)90139-8. [DOI] [PubMed] [Google Scholar]
  2. DE SOMER P., PRINZIE A., DENYS P., Jr, SCHONNE E. Mechanism of action of interferon. I. Relationship with viral ribonucleic acid. Virology. 1962 Jan;16:63–70. doi: 10.1016/0042-6822(62)90202-7. [DOI] [PubMed] [Google Scholar]
  3. Fantes K. H. Further purification of chick interferon. Nature. 1965 Sep 18;207(5003):1298–1298. doi: 10.1038/2071298a0. [DOI] [PubMed] [Google Scholar]
  4. Friedman R. M., Fantes K. H., Levy H. B., Carter W. B. Interferon action on parental Semliki forest virus ribonucleic acid. J Virol. 1967 Dec;1(6):1168–1173. doi: 10.1128/jvi.1.6.1168-1173.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Friedman R. M. Inhibition of arbovirus protein synthesis by interferon. J Virol. 1968 Oct;2(10):1081–1085. doi: 10.1128/jvi.2.10.1081-1085.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Friedman R. M., Levy H. B., Carter W. B. Replication of semliki forest virus: three forms of viral RNA produced during infection. Proc Natl Acad Sci U S A. 1966 Aug;56(2):440–446. doi: 10.1073/pnas.56.2.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Friedman R. M. Replicative intermediate of an arbovirus. J Virol. 1968 Jun;2(6):547–552. doi: 10.1128/jvi.2.6.547-552.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Friedman R. M., Sonnabend J. A. Inhibition of interferon action by puromycin. J Immunol. 1965 Oct;95(4):696–703. [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Mécs E., Sonnabend J. A., Martin E. M., Fantes K. H. The effect of interferon on the synthesis of RNA in chick cells infected with Semliki forest virus. J Gen Virol. 1967 Jan;1(1):25–40. doi: 10.1099/0022-1317-1-1-25. [DOI] [PubMed] [Google Scholar]
  11. Northrop R. L. Effect of puromycin and actinomycin D on a persistent mumps virus infection in vitro. J Virol. 1969 Aug;4(2):133–140. doi: 10.1128/jvi.4.2.133-140.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sreevalsan T., Yin F. H. Sindbis virus-induced viral ribonucleic acid polymerase. J Virol. 1969 Jun;3(6):599–604. doi: 10.1128/jvi.3.6.599-604.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tremblay G. Y., Daniels M. J., Schaechter M. Isolation of a cell membrane-DNA-nascent RNA complex from bacteria. J Mol Biol. 1969 Feb 28;40(1):65–76. doi: 10.1016/0022-2836(69)90296-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES