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. 1970 Aug;6(2):199–207. doi: 10.1128/jvi.6.2.199-207.1970

Virus-Specific Deoxyribonucleic Acid in Simian Virus 40-Exposed Hamster Cells: Correlation with S and T Antigens 1

Arthur S Levine 1,2,3, Michael N Oxman 1,2,3, Patrick H Henry 1,2,3, Myron J Levin 1,2,3, George T Diamandopoulos 1,2,3, John F Enders 1,2,3
PMCID: PMC376109  PMID: 4322872

Abstract

Several homologous hamster embryonic cell lines, transformed in association with simian virus (SV) 40 infection, were examined for the presence of deoxyribonucleic acid (DNA) complementary to SV40 ribonucleic acid (RNA) made in vitro. The methods employed permitted the detection of 10−5 μg of viral DNA in 100 μg of cellular DNA, corresponding to one-fifth of an SV40 DNA molecule per cell. Those lines which contained both the SV40 surface (S) and tumor (T) antigens also contained DNA complementary to SV40 RNA synthesized in vitro. In contrast, neither of two lines which contained S, but not T, antigen contained detectable DNA complementary to SV40 RNA. These findings suggest that the production of S antigen does not depend upon the persistence of SV40 DNA in transformed cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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