Abstract
The structure and morphogenesis of respiratory syncytial (RS) virus particles in a green monkey kidney cell line (Vero) were examined. Infected cells contained dense intracytoplasmic inclusions composed of filamentous structures. In places where inclusion material was associated with membranes, structural modifications were induced. There was a thickening of the membrane and an addition of projections 12 to 15 nm in length. The same changes were most frequently observed after association of isolated filamentous structures with the cytoplasmic membrane. The budding-off process was clearly visualized. The diameter of mature virus particles varied between 90 and 130 nm and that of the internal component varied between 11 and 15 nm. The similarities between ultrastructural features of cells infected with RS virus and pneumonia virus of mice are pointed out. It is proposed that these two viruses should be classified together in a third subgroup of myxoviruses.
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Selected References
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- BLOTH B. N., NORRBY E. C. MORPHOLOGY OF SUBUNITS OF RESPIRATORY SYNCYTIAL (RS) VIRUS. (BRIEF REPORT). Arch Gesamte Virusforsch. 1965;15:249–252. doi: 10.1007/BF01257736. [DOI] [PubMed] [Google Scholar]
- Bloth B., Norrby E. Electron microscopic analysis of the internal component of respiratory syncytial (RS) virus. Arch Gesamte Virusforsch. 1967;21(1):71–77. doi: 10.1007/BF01258478. [DOI] [PubMed] [Google Scholar]
- Bonissol C. Etude de cellules KB infectées par les Myxovirus parainfluenzae de type 2 et 3 et le virus respiratoire syncytial. II. Etude cytochimique. Ann Inst Pasteur (Paris) 1966 Sep;111(3):265–281. [PubMed] [Google Scholar]
- Compans R. W., Harter D. H., Choppin P. W. Studies on pneumonia virus of mice (PVM) in cell culture. II. Structure and morphogenesis of the virus particle. J Exp Med. 1967 Aug 1;126(2):267–276. doi: 10.1084/jem.126.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Compans R. W., Holmes K. V., Dales S., Choppin P. W. An electron microscopic study of moderate and virulent virus-cell interactions of the parainfluenza virus SV5. Virology. 1966 Nov;30(3):411–426. doi: 10.1016/0042-6822(66)90119-x. [DOI] [PubMed] [Google Scholar]
- Eckert E. A., Maassab H. F., Cavallaro J. J. Inner component of respiratory syncytial virus. J Bacteriol. 1965 Dec;90(6):1784–1785. doi: 10.1128/jb.90.6.1784-1785.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feller U., Dougherty R. M., Di Stefano H. S. Morphogenesis of Newcastle disease virus in chorioallantoic membrane. J Virol. 1969 Nov;4(5):753–762. doi: 10.1128/jvi.4.5.753-762.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Howe C., Morgan C., de Vaux St Cyr C., Hsu K. C., Rose H. M. Morphogenesis of type 2 parainfluenza virus examined by light and electron microscopy. J Virol. 1967 Feb;1(1):215–237. doi: 10.1128/jvi.1.1.215-237.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JORDAN W. S., Jr Growth characteristics of respiratory syncytial virus. J Immunol. 1962 May;88:581–590. [PubMed] [Google Scholar]
- Joncas J., Berthiaume L., Pavilanis V. The structure of the respiratory syncytial virus. Virology. 1969 Jul;38(3):493–496. doi: 10.1016/0042-6822(69)90166-4. [DOI] [PubMed] [Google Scholar]
- Prose P. H., Balk S. D., Liebhaber H., Krugman S. Studies of a myxovirus recovered from patients with infectious hepatitis. II. Fine structure and electron microscopic demonstration of intracytoplasmic internal component and viral filament formation. J Exp Med. 1965 Dec 1;122(6):1151–1160. doi: 10.1084/jem.122.6.1151. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zakstelskaya L. Y., Almeida J. D., Bradstreet C. M. The morphological characterisation of respiratory syncytial virus by a simple electron microscope technique. Acta Virol. 1967 Sep;11(5):420–423. [PubMed] [Google Scholar]