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. 2013 Aug 15;5(8):1991–2004. doi: 10.3390/v5081991

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Phylogenetic trees of PEDV strains generated by the neighbor-joining method with nucleotide sequences of the full-length spike genes. Bootstrapping with 1,000 replicates was performed to determine the percentage reliability for each internal node. PUR46-MAD is an out group control. Horizontal branch lengths are proportional to genetic distances between PEDV strains. Black circles indicate PEDV isolates from the 2012 outbreak in Gansu Province, China. Scale bar indicates nucleotide substitutions per site.