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. 1970 Oct;6(4):414–420. doi: 10.1128/jvi.6.4.414-420.1970

Density Gradient Centrifugation Studies on Lymphocytic Choriomeningitis Virus and on Viral Ribonucleic Acid

Ib R Pedersen 1
PMCID: PMC376138  PMID: 4993891

Abstract

Lymphocytic choriomeningitis (LCM) virus, Traub strain, was purified from BHK-21 tissue culture medium. The virus was then analyzed by equilibrium centrifugation and rate zonal centrifugation in sucrose gradients. A buoyant density in sucrose of 1.18 g/ml was found and the S20, w value was estimated to be about 470 to 500S. Furthermore, the 3H-uridine-labeled ribonucleic acid (RNA) from virus was extracted from LCM virus and analyzed by rate zonal centrifugation. Two major and one minor single-stranded RNA components were found with sedimentation coefficients of 28, 22, and 18S.

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Selected References

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