Abstract
Tail plates obtained from T4D amber mutants were examined with respect to sedimentation behavior, subunit molecular weights, amino acid composition, isoelectric points, and morphology. Intact plates had an S20,w of 77S from pH 5 to 9. The only conformational change noted was that below pH 5 tail plates readily dimerized yielding vis-à-vis dimers with an S20,w of 124S. Dissociated plates consisted of three major proteins with molecular weights of 53 K ± 5, 31 K ± 3, and 17 K ± 2 daltons. The amino acid analyses indicated that plates had a composition distinct from fibers and tubes and were relatively rich in tryptophan. Degradation studies with dimethyl sulfoxide (DMSO) indicated that tail plates had a unique biological structure. After treatment with DMSO, and to some extent without DMSO, or from lysates of defective mutants, tetrad structures were observed in the electron microscope. These structures had an amino acid content and relative amounts of types of subunits similar but not identical to intact plates. It was proposed that plates were composed of nine such tetrads giving rise to a structure with six- and threefold symmetry.
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- Anderson T. F., Stephens R. Decomposition of T6 bacteriophage in alkaline solutions. Virology. 1964 May;23(1):113–117. doi: 10.1016/s0042-6822(64)80017-9. [DOI] [PubMed] [Google Scholar]
- Cummings D. J., Chapman V. A., DeLong S. S. Disruption of T-even bacteriophages by dimethyl sulfoxide. J Virol. 1968 Jun;2(6):610–620. doi: 10.1128/jvi.2.6.610-620.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cummings D. J., Kusy A. R., Chapman V. A., DeLong S. S., Stone K. R. Characterization of T-even bacteriophage substructures. I. Tail fibers and tail tubes. J Virol. 1970 Oct;6(4):534–544. doi: 10.1128/jvi.6.4.534-544.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davison P. F. Proteins in denaturing solvents: gel exclusion studies. Science. 1968 Aug 30;161(3844):906–907. doi: 10.1126/science.161.3844.906. [DOI] [PubMed] [Google Scholar]
- Edgar R. S., Lielausis I. Some steps in the assembly of bacteriophage T4. J Mol Biol. 1968 Mar 14;32(2):263–276. doi: 10.1016/0022-2836(68)90008-9. [DOI] [PubMed] [Google Scholar]
- Forrest G. L., Cummings D. J. Head proteins from T-even bacteriophage. I. Molecular weight characterization. J Virol. 1970 Mar;5(3):398–405. doi: 10.1128/jvi.5.3.398-405.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KOZLOFF L. M., LUTE M., HENDERSON K. Viral invasion. I. Rupture of thiol ester bonds in the bacteriophage tail. J Biol Chem. 1957 Sep;228(1):511–528. [PubMed] [Google Scholar]
- Simon L. D., Anderson T. F. The infection of Escherichia coli by T2 and T4 bacteriophages as seen in the electron microscope. I. Attachment and penetration. Virology. 1967 Jun;32(2):279–297. doi: 10.1016/0042-6822(67)90277-2. [DOI] [PubMed] [Google Scholar]
- Simon L. D., Anderson T. F. The infection of Escherichia coli by T2 and T4 bacteriophages as seen in the electron microscope. II. Structure and function of the baseplate. Virology. 1967 Jun;32(2):298–305. doi: 10.1016/0042-6822(67)90278-4. [DOI] [PubMed] [Google Scholar]
- To C. M., Eisenstark A., Töreci H. Structure of mutator phage Mu1 of Escherichia coli. J Ultrastruct Res. 1966 Mar;14(5):441–448. doi: 10.1016/s0022-5320(66)80074-6. [DOI] [PubMed] [Google Scholar]
- To C. M., Kellenberger E., Eisenstark A. Disassembly of T-even bacteriophage into structural parts and subunits. J Mol Biol. 1969 Dec 28;46(3):493–511. doi: 10.1016/0022-2836(69)90192-2. [DOI] [PubMed] [Google Scholar]