Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1970 Nov;6(5):599–603. doi: 10.1128/jvi.6.5.599-603.1970

Propagation in Human Cells of a Filterable Agent from the ST Feline Sarcoma

R Shihman Chang 1, H Dean Golden 1, Boyd Harrold 1
PMCID: PMC376164  PMID: 4098429

Abstract

Nineteen lines of human fibroblasts were inoculated with a filtrate prepared from the ST feline sarcoma. Seven lines showed morphological alteration and released focus-forming activity for feline cells, 2 lines showed morphological alteration but did not release focus-forming activity, and 11 lines showed no morphological alteration and released no focus-forming agent. Morphologically altered cells appeared enlarged, hyper-refractile, and intensely stained by hematoxylin. They neither assumed a crisscross pattern nor piled up to form a visible focus. Time-lapse cinegraph showed that the morphologically altered cells did not divide and were motile. The fluid from two human fibroblast cultures, inoculated 4 and 14 weeks previously with the ST sarcoma filtrate, induced fibrosarcoma in newborn kittens.

Full text

PDF
599

Images in this article

600Image
on p.600

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler H. E., DaMassa A. J. Effect of dextrose in medium for the preparation of Mycoplasma gallisepticum plate antigens. Appl Microbiol. 1968 Apr;16(4):558–562. doi: 10.1128/am.16.4.558-562.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Deinhardt F., Wolfe L. G., Theilen G. H., Snyder S. P. ST-feline fibrosarcoma virus: induction of tumors in marmoset monkeys. Science. 1970 Feb 6;167(3919):881–881. doi: 10.1126/science.167.3919.881. [DOI] [PubMed] [Google Scholar]
  3. Dougherty R. M., Di Stefano H. S. Virus particles associated with "nonproducer" Rous sarcoma cells. Virology. 1965 Nov;27(3):351–359. doi: 10.1016/0042-6822(65)90115-7. [DOI] [PubMed] [Google Scholar]
  4. Fischinger P. J., O'Connor T. E. Productive infection and morphologic alteration of human cells by a modified sarcoma virus. J Natl Cancer Inst. 1970 Feb;44(2):429–438. [PubMed] [Google Scholar]
  5. GERBER P. Tumors induced in hamsters by simian virus 40: persistent subviral infection. Science. 1963 May 24;140(3569):889–890. doi: 10.1126/science.140.3569.889. [DOI] [PubMed] [Google Scholar]
  6. Gerber P. Studies on the transfer of subviral infectivity from SV40-induced hamster tumor cells to indicator cells. Virology. 1966 Apr;28(4):501–509. doi: 10.1016/0042-6822(66)90234-0. [DOI] [PubMed] [Google Scholar]
  7. Hardy W. D., Jr, Geering G., Old L. J., DE Harven E., Brody R. S., McDonough S. Feline leukemia virus: occurrence of viral antigen in the tissues of cats with lymphosarcoma and other diseases. Science. 1969 Nov 21;166(3908):1019–1021. doi: 10.1126/science.166.3908.1019. [DOI] [PubMed] [Google Scholar]
  8. Huebner R. J., Hartley J. W., Rowe W. P., Lane W. T., Capps W. I. Rescue of the defective genome of Moloney sarcoma virus from a noninfectious hamster tumor and the production of pseudotype sarcoma viruses with various murine leukemia viruses. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1164–1169. doi: 10.1073/pnas.56.4.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jarrett O., Laird H. M., Hay D., Crighton G. W. Replication of cat leukemia virus in cell cultures. Nature. 1968 Aug 3;219(5153):521–522. doi: 10.1038/219521a0. [DOI] [PubMed] [Google Scholar]
  10. Jarrett O., Laird H. M., Hay D. Growth of feline leukaemia virus in human cells. Nature. 1969 Dec 20;224(5225):1208–1209. doi: 10.1038/2241208a0. [DOI] [PubMed] [Google Scholar]
  11. McKissick G. E., Lamont P. H. Characteristics of a virus isolated from a feline fibrosarcoma. J Virol. 1970 Feb;5(2):247–257. doi: 10.1128/jvi.5.2.247-257.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sarma P. S., Huebner R. J., Basker J. F., Vernon L., Gilden R. V. Feline leukemia and sarcoma viruses: susceptibility of human cells to infection. Science. 1970 May 29;168(3935):1098–1100. doi: 10.1126/science.168.3935.1098. [DOI] [PubMed] [Google Scholar]
  13. Snyder S. P., Theilen G. H. Transmissible feline fibrosarcoma. Nature. 1969 Mar 15;221(5185):1074–1075. doi: 10.1038/2211074a0. [DOI] [PubMed] [Google Scholar]
  14. Vogt P. K. A virus released by "nonproducing" Rous sarcoma cells. Proc Natl Acad Sci U S A. 1967 Sep;58(3):801–808. doi: 10.1073/pnas.58.3.801. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES