Abstract
A method was devised for obtaining single cell clones of human leukocytic cell lines in the presence of a human placental cell feeder layer. Clones of two lines, LS-B and EB3, which contain Epstein-Barr viral (EBV) antigen in approximately 1% of cells were tested for EBV antigen. Since all EB3 clones and LS-B clones contained EBV antigen, it is concluded that in vitro EBV genome is associated with all of the cells.
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Selected References
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- Epstein M. A., Achong B. G. Specific immunofluorescence test for the herpes-type EB virus of Burkitt lymphoblasts, authenticated by electron microscopy. J Natl Cancer Inst. 1968 Mar;40(3):593–607. [PubMed] [Google Scholar]
- Epstein M. A., Barr Y. M., Achong B. G. Studies with Burkitt's lymphoma. Wistar Inst Symp Monogr. 1965 Sep;4:69–82. [PubMed] [Google Scholar]
- Grogan E. A., Enders J. F., Miller G. Trypsinized placental cell cultures for the propagation of viruses and as "feeder layers". J Virol. 1970 Mar;5(3):406–409. doi: 10.1128/jvi.5.3.406-409.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Henle G., Henle W. Immunofluorescence in cells derived from Burkitt's lymphoma. J Bacteriol. 1966 Mar;91(3):1248–1256. doi: 10.1128/jb.91.3.1248-1256.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller G., Enders J. F., Lisco H., Kohn H. I. Establishment of lines from normal human blood leukocytes by co-cultivation with a leukocyte line derived from a leukemic child. Proc Soc Exp Biol Med. 1969 Oct;132(1):247–252. doi: 10.3181/00379727-132-34189. [DOI] [PubMed] [Google Scholar]