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. 1970 Dec;6(6):707–716. doi: 10.1128/jvi.6.6.707-716.1970

Electrophoretic Distribution of the Proteins and Glycoproteins of Influenza Virus and Sendai Virus

Jean Content 1,1, Peter H Duesberg 1
PMCID: PMC376186  PMID: 4322079

Abstract

The proteins of influenza (WSN) and Sendai virus have been separated by polyacrylamide gel electrophoresis into five components. In both cases, three of these components were shown to be glycoproteins containing fucose, galactose, and glucosamine. Two protein components of each virus were probably free from these sugar residues, including the structural unit of the viral ribonucleoprotein (molecular weight of about 60,000 daltons).

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Selected References

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  1. ADA G. L., GOTTSCHALK A. The component sugars of the influenza-virus particle. Biochem J. 1956 Apr;62(4):686–689. doi: 10.1042/bj0620686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bikel I., Duesberg P. H. Proteins of Newcastle disease virus and of the viral nucleocapsid. J Virol. 1969 Oct;4(4):388–393. doi: 10.1128/jvi.4.4.388-393.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blair C. D., Robinson W. S. Replication of Sendai virus. I. Comparison of the viral RNA and virus-specific RNA synthesis with Newcastle disease virus. Virology. 1968 Aug;35(4):537–549. doi: 10.1016/0042-6822(68)90284-5. [DOI] [PubMed] [Google Scholar]
  4. Blair C. D., Robinson W. S. Replication of Sendai virus. II. Steps in virus assembly. J Virol. 1970 May;5(5):639–650. doi: 10.1128/jvi.5.5.639-650.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burge B. W., Huang A. S. Comparison of membrane protein glycopeptides of Sindbis virus and vesicular stomatitis virus. J Virol. 1970 Aug;6(2):176–182. doi: 10.1128/jvi.6.2.176-182.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Caliguiri L. A., Klenk H. D., Choppin P. W. The proteins of the parainfluenza virus SV5. 1. Separation of virion polypeptides by polyacrylamide gel electrophoresis. Virology. 1969 Nov;39(3):460–466. doi: 10.1016/0042-6822(69)90094-4. [DOI] [PubMed] [Google Scholar]
  7. Choppin P. W. Replication of influenza virus in a continuous cell line: high yield of infective virus from cells inoculated at high multiplicity. Virology. 1969 Sep;39(1):130–134. doi: 10.1016/0042-6822(69)90354-7. [DOI] [PubMed] [Google Scholar]
  8. Deusberg P. H., Robinson W. S. On the structure and replication of influenza virus. J Mol Biol. 1967 May 14;25(3):383–405. doi: 10.1016/0022-2836(67)90193-3. [DOI] [PubMed] [Google Scholar]
  9. Dimmock N. J. New virus-specific antigens in cells infected with influenza virus. Virology. 1969 Oct;39(2):224–234. doi: 10.1016/0042-6822(69)90042-7. [DOI] [PubMed] [Google Scholar]
  10. Duesberg P. H. Distinct subunits of the ribonucleoprotein of influenza virus. J Mol Biol. 1969 Jun 28;42(3):485–499. doi: 10.1016/0022-2836(69)90237-x. [DOI] [PubMed] [Google Scholar]
  11. Duesberg P. H., Martin G. S., Vogt P. K. Glycoprotein components of avian and murine RNA tumor viruses. Virology. 1970 Aug;41(4):631–646. doi: 10.1016/0042-6822(70)90428-9. [DOI] [PubMed] [Google Scholar]
  12. Duesberg P. H., Robinson H. L., Robinson W. S., Huebner R. J., Turner H. C. Proteins of Rous sarcoma virus. Virology. 1968 Sep;36(1):73–86. doi: 10.1016/0042-6822(68)90118-9. [DOI] [PubMed] [Google Scholar]
  13. Evans M. J., Kingsbury D. W. Separation of Newcastle disease virus proteins by polyacrylamide gel electrophoresis. Virology. 1969 Apr;37(4):597–604. doi: 10.1016/0042-6822(69)90277-3. [DOI] [PubMed] [Google Scholar]
  14. FRISCH-NIGGEMEYER W., HOYLE L. The nucleic acid and carbohydrate content of influenza virus A and of virus fractions produced by ether disintegration. J Hyg (Lond) 1956 Jun;54(2):201–212. doi: 10.1017/s0022172400044466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. FROMMHAGEN L. H., KNIGHT C. A., FREEMAN N. K. The ribonucleic acid, lipid, and polysaccharide constituents of influenza virus preparations. Virology. 1959 Jun;8(2):176–197. doi: 10.1016/0042-6822(59)90003-0. [DOI] [PubMed] [Google Scholar]
  16. HARBOE A. THE NORMAL ALLANTOIC ANTIGEN WHICH NEUTRALIZES THE INFLUENZA VIRUS HI-ANTIBODY TO HOST MATERIAL. Acta Pathol Microbiol Scand. 1963;57:488–492. doi: 10.1111/j.1699-0463.1963.tb05116.x. [DOI] [PubMed] [Google Scholar]
  17. Haslam E. A., Cheyne I. M., White D. O. The structural proteins of Newcastle disease virus. Virology. 1969 Sep;39(1):118–129. doi: 10.1016/0042-6822(69)90353-5. [DOI] [PubMed] [Google Scholar]
  18. Holland J. J., Kiehn E. D. Influenza virus effects on cell membrane proteins. Science. 1970 Jan 9;167(3915):202–205. doi: 10.1126/science.167.3915.202. [DOI] [PubMed] [Google Scholar]
  19. Joss A., Gandhi S. S., Hay A. J., Burke D. C. Ribonucleic Acid and protein synthesis in chick embryo cells infected with fowl plague virus. J Virol. 1969 Dec;4(6):816–822. doi: 10.1128/jvi.4.6.816-822.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Klenk H. D., Choppin P. W. Chemical composition of the parainfluenza virus SV5. Virology. 1969 Jan;37(1):155–157. doi: 10.1016/0042-6822(69)90321-3. [DOI] [PubMed] [Google Scholar]
  21. LAVER W. G. STRUCTURAL STUDIES ON THE PROTEIN SUBUNITS FROM THREE STRAINS OF INFLUENZA VIRUS. J Mol Biol. 1964 Jul;9:109–124. doi: 10.1016/s0022-2836(64)80094-2. [DOI] [PubMed] [Google Scholar]
  22. Laver W. G., Webster R. G. The structure of influenza viruses. IV. Chemical studies of the host antigen. Virology. 1966 Sep;30(1):104–115. doi: 10.1016/s0042-6822(66)81014-0. [DOI] [PubMed] [Google Scholar]
  23. Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
  24. Spear P. G., Kellejmroian B. Proteins spcified by herpes simplex virus. II. Viral glycoprotins associated with cellular membranes. J Virol. 1970 Feb;5(2):123–131. doi: 10.1128/jvi.5.2.123-131.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Strauss J. H., Jr, Burge B. W., Darnell J. E. Carbohydrate content of the membrane protein of Sindbis virus. J Mol Biol. 1970 Feb 14;47(3):437–448. doi: 10.1016/0022-2836(70)90313-x. [DOI] [PubMed] [Google Scholar]
  26. Taylor J. M., Hampson A. W., White D. O. The polypeptides of influenza virus. 1. Cytoplasmic synthesis and nuclear accumulation. Virology. 1969 Nov;39(3):419–425. doi: 10.1016/0042-6822(69)90090-7. [DOI] [PubMed] [Google Scholar]
  27. WATERSON A. P. Two kinds of myxovirus. Nature. 1962 Mar 24;193:1163–1164. doi: 10.1038/1931163a0. [DOI] [PubMed] [Google Scholar]

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