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. 1971 Oct;8(4):355–362. doi: 10.1128/jvi.8.4.355-362.1971

Sensitivity of Intracellular Bacteriophage λ to Colicin CA42-E2

Ron R Hull 1,1, Peter Reeves 1
PMCID: PMC376208  PMID: 4943074

Abstract

Treatment of Escherichia coli K-12 infected by λ CIts857 with colicin CA42-E2 resulted in partial inhibition of the infectious process. Uninfected bacteria were killed by colicin with a probability of about five times that with which similarly treated λ-infected bacteria lose plaque-forming ability. The λ deoxyribonucleic acid (DNA), when present in a bacterial cell either as the replicating DNA of infectious phage or as the nonreplicating DNA of superinfecting phage, was degraded to acid-soluble material after colicin treatment. Analysis of the intermediates of DNA breakdown has revealed that degradation of the DNA to acid-soluble material is preceded by endonucleolytic fragmentation of the chromosome at a limited number of sites. This is the same mechanism of degradation previously observed for E. coli DNA after colicin treatment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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