Abstract
We have studied the effect of the drug pactamycin on protein synthesis in poliovirus-infected HeLa cells. At a concentration which primarily inhibits initiation of protein synthesis, the spectrum of poliovirus proteins synthesized is markedly changed. The amount of NCVP 1, the capsid precursor, is greatly reduced relative to NCVP 2 and the amount of NCVP X is slightly reduced. Since it is believed that there is only one major site for the initiation of protein synthesis on the poliovirus genome, we interpret this differential effect on the poliovirus proteins to be an indication of their relative distance from the initiation site. On this basis, we propose a gene order for the poliovirus genome (5′ → 3′) of NCVP 1, NCVP X, NCVP 2.
Full text
PDF![395](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b453/376212/dc46bc45fca3/jvirol00286-0041.png)
![396](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b453/376212/665832c646b2/jvirol00286-0042.png)
![397](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b453/376212/7cf1514575b9/jvirol00286-0043.png)
![398](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b453/376212/6f75e16d36e1/jvirol00286-0044.png)
![399](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b453/376212/1c82809fe386/jvirol00286-0045.png)
![400](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b453/376212/3d8151581800/jvirol00286-0046.png)
![401](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b453/376212/cf220b1c8a89/jvirol00286-0047.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baltimore D., Girard M., Darnell J. E. Aspects of the synthesis of poliovirus RNA and the formation of virus particles. Virology. 1966 Jun;29(2):179–189. doi: 10.1016/0042-6822(66)90024-9. [DOI] [PubMed] [Google Scholar]
- Brown J. C., Smith A. E. Initiator codons in eukaryotes. Nature. 1970 May 16;226(5246):610–612. doi: 10.1038/226610a0. [DOI] [PubMed] [Google Scholar]
- Cohen L. B., Goldberg I. H., Herner A. E. Inhibition by pactamycin of the initiation of protein synthesis. Effect on the 30S ribosomal subunit. Biochemistry. 1969 Apr;8(4):1327–1335. doi: 10.1021/bi00832a005. [DOI] [PubMed] [Google Scholar]
- Cohen L. B., Herner A. E., Goldberg I. H. Inhibition by pactamycin of the initiation of protein synthesis. Binding of N-acetylphenylalanyl transfer ribonucleic acid and polyuridylic acid to ribosomes. Biochemistry. 1969 Apr;8(4):1312–1326. doi: 10.1021/bi00832a004. [DOI] [PubMed] [Google Scholar]
- Colombo B., Felicetti L., Baglioni C. Inhibition of protein synthesis in reticulocytes by antibiotics. I. Effects on polysomes. Biochim Biophys Acta. 1966 Apr 18;119(1):109–119. doi: 10.1016/0005-2787(66)90043-8. [DOI] [PubMed] [Google Scholar]
- EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
- Felicetti L., Colombo B., Baglioni C. Inhibition of protein synthesis in reticulocytes by antibiotics. II. The site of action of cycloheximide, streptovitacin A and pactamycin. Biochim Biophys Acta. 1966 Apr 18;119(1):120–129. [PubMed] [Google Scholar]
- Granboulan N., Girard M. Molecular weight of poliovirus ribonucleic acid. J Virol. 1969 Oct;4(4):475–479. doi: 10.1128/jvi.4.4.475-479.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huang A. S., Balitmore D. Initiation of polyribosome formation in poliovirus-infected HeLa cells. J Mol Biol. 1970 Feb 14;47(3):275–291. doi: 10.1016/0022-2836(70)90302-5. [DOI] [PubMed] [Google Scholar]
- Jacobson M. F., Asso J., Baltimore D. Further evidence on the formation of poliovirus proteins. J Mol Biol. 1970 May 14;49(3):657–669. doi: 10.1016/0022-2836(70)90289-5. [DOI] [PubMed] [Google Scholar]
- Jacobson M. F., Baltimore D. Polypeptide cleavages in the formation of poliovirus proteins. Proc Natl Acad Sci U S A. 1968 Sep;61(1):77–84. doi: 10.1073/pnas.61.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kiehn E. D., Holland J. J. Membrane and nonmembrane proteins of mammalian cells. Synthesis, turnover, and size distribution. Biochemistry. 1970 Apr 14;9(8):1716–1728. doi: 10.1021/bi00810a010. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Macdonald J. S., Goldberg I. H. An effect of pactamycin on the initiation of protein synthesis in reticulocytes. Biochem Biophys Res Commun. 1970 Oct 9;41(1):1–8. doi: 10.1016/0006-291x(70)90460-2. [DOI] [PubMed] [Google Scholar]
- PENMAN S., BECKER Y., DARNELL J. E. A CYTOPLASMIC STRUCTURE INVOLVED IN THE SYNTHESIS AND ASSEMBLY OF POLIOVIRUS COMPONENTS. J Mol Biol. 1964 Apr;8:541–555. doi: 10.1016/s0022-2836(64)80010-3. [DOI] [PubMed] [Google Scholar]
- SANGER F., BRETSCHER M. S., HOCQUARD E. J. A STUDY OF THE PRODUCTS FROM A POLYNUCLEOTIDE-DIRECTED CELL-FREE PROTEIN SYNTHESIZING SYSTEM. J Mol Biol. 1964 Jan;8:38–45. doi: 10.1016/s0022-2836(64)80146-7. [DOI] [PubMed] [Google Scholar]
- Scheele C. M., Pfefferkorn E. R. Virus-specific proteins synthesized in cells infected with RNA+ temperature-sensitive mutants of Sindbis virus. J Virol. 1970 Mar;5(3):329–337. doi: 10.1128/jvi.5.3.329-337.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Summers D. F., Maizel J. V., Jr, Darnell J. E., Jr Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Aug;54(2):505–513. doi: 10.1073/pnas.54.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]