Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1971 Oct;8(4):409–416. doi: 10.1128/jvi.8.4.409-416.1971

Enzymes and Nucleotides in Virions of Rous Sarcoma Virus

Satoshi Mizutani 1, Howard M Temin 1
PMCID: PMC376214  PMID: 4331649

Abstract

In addition to the previously described deoxyribonucleic acid (DNA) polymerase, DNA ligase, DNA exonuclease, and DNA endonuclease activities, purified virions of Schmidt-Ruppin strain of Rous sarcoma virus (SRV) have nucleotides and nucleotide kinase, phosphatase, hexokinase, and lactate dehydrogenase activities. The SRV virions have no glucose-6-phosphate dehydrogenase activity. All enzyme activities, but glucose-6-phosphate dehydrogenase and adenosine triphosphatase, were increased by disruption of the virions. The DNA polymerase, DNA ligase, and hexokinase activities had a higher specific activity in purified virion cores. It is suggested that during assembly virions of SRV may pick up cytoplasmic components which bind to virion proteins. The role of these components in viral replication is not known at present.

Full text

PDF
409

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altaner C., Temin H. M. Carcinogenesis by RNA sarcoma viruses. XII. A quantitative study of infection of rat cells in vitro by avian sarcoma viruses. Virology. 1970 Jan;40(1):118–134. doi: 10.1016/0042-6822(70)90384-3. [DOI] [PubMed] [Google Scholar]
  2. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baril E. F., Jenkins M. D., Brown O. E., Laszlo J. DNA polymerase activities associated with smooth membranes and ribosomes from rat liver and hepatoma cytoplasm. Science. 1970 Jul 3;169(3940):87–89. doi: 10.1126/science.169.3940.87. [DOI] [PubMed] [Google Scholar]
  4. Bishop J. M., Levinson W. E., Quintrell N., Sullivan D., Fanshier L., Jackson J. The low molecular weight RNAs of Rous sarcoma virus. I. The 4 S RNA. Virology. 1970 Sep;42(1):182–195. doi: 10.1016/0042-6822(70)90251-5. [DOI] [PubMed] [Google Scholar]
  5. Borsa J., Graham A. F. Reovirus: RNA polymerase activity in purified virions. Biochem Biophys Res Commun. 1968 Dec 30;33(6):895–901. doi: 10.1016/0006-291x(68)90396-3. [DOI] [PubMed] [Google Scholar]
  6. Borsa J., Grover J., Chapman J. D. Presence of nucleoside triphosphate phosphohydrolase activity in purified virions of reovirus. J Virol. 1970 Sep;6(3):295–302. doi: 10.1128/jvi.6.3.295-302.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carnegie J. W., Deeney A. O., Olson K. C., Beaudreau G. S. An RNA fraction from myeloblastosis virus having properties similar to transfer RNA. Biochim Biophys Acta. 1969 Oct 22;190(2):274–284. doi: 10.1016/0005-2787(69)90079-3. [DOI] [PubMed] [Google Scholar]
  8. Cashel M., Lazzarini R. A., Kalbacher B. An improved method for thin-layer chromatography of nucleotide mixtures containing 32P-labelled orthophosphate. J Chromatogr. 1969 Mar 11;40(1):103–109. doi: 10.1016/s0021-9673(01)96624-5. [DOI] [PubMed] [Google Scholar]
  9. Chow N. L., Simpson R. W. RNA-dependent RNA polymerase activity associated with virions and subviral particles of myxoviruses. Proc Natl Acad Sci U S A. 1971 Apr;68(4):752–756. doi: 10.1073/pnas.68.4.752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Coffin J. M., Temin H. M. Comparison of Rous sarcoma virus-specific deoxyribonucleic acid polymerases in virions of Rous sarcoma virus and in Rous sarcoma virus-infected chicken cells. J Virol. 1971 May;7(5):625–634. doi: 10.1128/jvi.7.5.625-634.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Erikson E., Erikson R. L. Isolation of amino acid acceptor RNA from purified avian myeloblastosis virus. J Mol Biol. 1970 Sep 14;52(2):387–390. doi: 10.1016/0022-2836(70)90038-0. [DOI] [PubMed] [Google Scholar]
  12. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  13. GREEN I., BEARD J. W. Virus of avian erythromyeoloblastic leukosis. VI. Properties of the enzyme associated with the virus in dephosphorylating adenosine triphosphate. J Natl Cancer Inst. 1955 Apr;15(5):1217–1229. [PubMed] [Google Scholar]
  14. Gold P. H., Dales S. Localization of nucleotide phosphohydrolase activity within vaccinia. Proc Natl Acad Sci U S A. 1968 Jul;60(3):845–852. doi: 10.1073/pnas.60.3.845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Huang A. S., Baltimore D., Bratt M. A. Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase. J Virol. 1971 Mar;7(3):389–394. doi: 10.1128/jvi.7.3.389-394.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ives D. H., Durham J. P., Tucker V. S. Rapid determination of nucleoside kinase and nucleotidase activities with tritium-labeled substrates. Anal Biochem. 1969 Apr 4;28(1):192–205. doi: 10.1016/0003-2697(69)90170-5. [DOI] [PubMed] [Google Scholar]
  17. Kapuler A. M., Mendelsohn N., Klett H., Acs G. Four base-specific nucleoside 5'-triphosphatases in the subviral core of reovirus. Nature. 1970 Mar 28;225(5239):1209–1213. doi: 10.1038/2251209a0. [DOI] [PubMed] [Google Scholar]
  18. Kates J. R., McAuslan B. R. Messenger RNA synthesis by a "coated" viral genome. Proc Natl Acad Sci U S A. 1967 Feb;57(2):314–320. doi: 10.1073/pnas.57.2.314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lewandowski L. J., Kalmakoff J., Tanada Y. Characterization of a Ribonucleic Acid Polymerase Activity Associated with Purified Cytoplasmic Polyhedrosis Virus of the Silkworm Bombyx mori. J Virol. 1969 Dec;4(6):857–865. doi: 10.1128/jvi.4.6.857-865.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mizutani S., Boettiger D., Temin H. M. A DNA-depenent DNA polymerase and a DNA endonuclease in virions of Rous sarcoma virus. Nature. 1970 Oct 31;228(5270):424–427. doi: 10.1038/228424a0. [DOI] [PubMed] [Google Scholar]
  21. Mizutani S., Temin H. M., Kodama M., Wells R. T. DNA ligase and exonuclease activities in virions of rous sarcoma virus. Nat New Biol. 1971 Apr 21;230(16):232–235. doi: 10.1038/newbio230232a0. [DOI] [PubMed] [Google Scholar]
  22. Munyon W., Paoletti E., Grace J. T., Jr RNA polymerase activity in purified infectious vaccinia virus. Proc Natl Acad Sci U S A. 1967 Dec;58(6):2280–2287. doi: 10.1073/pnas.58.6.2280. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Neuhard J. Studies on the acid-soluble nucleotide pool in thymine-requiring mutants of Escherichia coli during thymine starvation. 3. On the regulation of the deoxyadenosine triphosphate and deoxycytidine triphosphate pools of Escherichia coli. Biochim Biophys Acta. 1966 Oct 24;129(1):104–115. doi: 10.1016/0005-2787(66)90012-8. [DOI] [PubMed] [Google Scholar]
  24. Penhoet E., Miller H., Doyle M., Blatti S. RNA-dependent RNA polymerase activity in influenza virions. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1369–1371. doi: 10.1073/pnas.68.6.1369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Pogo B. G., Dales S. Regulation of the synthesis of nucleotide phosphohydrolase and neutral deoxyribonuclease: two activities present within purified vaccina virus. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1297–1303. doi: 10.1073/pnas.63.4.1297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Pogo B. G., Dales S. Two deoxyribonuclease activities within purified vaccinia virus. Proc Natl Acad Sci U S A. 1969 Jul;63(3):820–827. doi: 10.1073/pnas.63.3.820. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Randerath K., Randerath E. Ion-exchange thin-layer chromatography. XIV. Separation of nucleotide sugars and nucleoside monophosphates on PEI-cellulose. Anal Biochem. 1965 Dec;13(3):575–579. doi: 10.1016/0003-2697(65)90356-8. [DOI] [PubMed] [Google Scholar]
  28. Roy P., Bishop D. H. Nucleoside triphosphate phosphotransferase. A new enzyme activity of oncogenic and non-oncogenic "budding" viruses. Biochim Biophys Acta. 1971 Apr 14;235(1):191–206. doi: 10.1016/0005-2744(71)90047-7. [DOI] [PubMed] [Google Scholar]
  29. Salzman L. A. DNA polymerase activity associated with purified Kilham rat virus. Nat New Biol. 1971 Jun 9;231(23):174–176. doi: 10.1038/newbio231174a0. [DOI] [PubMed] [Google Scholar]
  30. Shatkin A. J., Sipe J. D. RNA polymerase activity in purified reoviruses. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1462–1469. doi: 10.1073/pnas.61.4.1462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Temin H. M. Malignant transformation of cells by viruses. Perspect Biol Med. 1970 Autumn;14(1):11–26. doi: 10.1353/pbm.1970.0006. [DOI] [PubMed] [Google Scholar]
  32. Temin H. M. Mechanism of cell transformation by RNA tumor viruses. Annu Rev Microbiol. 1971;25:609–648. doi: 10.1146/annurev.mi.25.100171.003141. [DOI] [PubMed] [Google Scholar]
  33. Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]
  34. Temin H. M. Studies on carcinogenesis by avian sarcoma viruses. 8. Glycolysis and cell multiplication. Int J Cancer. 1968 Mar 15;3(2):273–282. doi: 10.1002/ijc.2910030213. [DOI] [PubMed] [Google Scholar]
  35. Trávnícek M., Ríman J. Chromatographic differences between lysyl-tRNA's from avian tumor virus BAI strain A and virus transformed cells. Biochim Biophys Acta. 1970 Jan 21;199(1):283–285. [PubMed] [Google Scholar]
  36. Vilagines R., McAuslan B. R. Proteins of polyhedal cytoplasmic deoxyvirus. II. Nucleotide phosphohydrolase activity associated with frog virus 3. J Virol. 1971 May;7(5):619–624. doi: 10.1128/jvi.7.5.619-624.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES