Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1971 Oct;8(4):597–600. doi: 10.1128/jvi.8.4.597-600.1971

Repair Replication of HeLa Cell Deoxyribonucleic Acid in Cells Infected with Newcastle Disease Virus or Mengovirus or Treated with Puromycin

Roger Hand 1, Igor Tamm 1
PMCID: PMC376235  PMID: 4331658

Abstract

We examined repair replication of HeLa cell deoxyribonucleic acid (DNA) in cells infected with mengovirus or Newcastle disease virus or treated with puromycin. Cellular DNA was damaged by ultraviolet light and then pulse-labeled with 3H-thymidine. Autoradiographic analysis of non-S-phase DNA synthesis (repair replication) showed that there was no inhibition of this process at a time when overall cellular DNA synthesis was severely inhibited by either virus infection or puromycin treatment.

Full text

PDF
597

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amako K., Dales S. Cytopathology of Mengovirus infection. I. Relationship between cellular disintegration and virulence. Virology. 1967 Jun;32(2):184–200. doi: 10.1016/0042-6822(67)90269-3. [DOI] [PubMed] [Google Scholar]
  2. BABLANIAN R., EGGERS H. J., TAMM I. STUDIES ON THE MECHANISM OF POLIOVIRUS-INDUCED CELL DAMAGE. I. THE RELATION BETWEEN POLIOVIRUS,-INDUCED METABOLIC AND MORPHOLOGICAL ALTERATIONS IN CULTURED CELLS. Virology. 1965 May;26:100–113. doi: 10.1016/0042-6822(65)90030-9. [DOI] [PubMed] [Google Scholar]
  3. Cleaver J. E. Defective repair replication of DNA in xeroderma pigmentosum. Nature. 1968 May 18;218(5142):652–656. doi: 10.1038/218652a0. [DOI] [PubMed] [Google Scholar]
  4. Cleaver J. E. Repair replication of mammalian cell DNA: effects of compounds that inhibit DNA synthesis or dark repair. Radiat Res. 1969 Feb;37(2):334–348. [PubMed] [Google Scholar]
  5. Cleaver J. R., Painter R. B. Evidence for repair replication of HeLa cell DNA damaged by ultraviolet light. Biochim Biophys Acta. 1968 Jul 23;161(2):552–554. doi: 10.1016/0005-2787(68)90131-7. [DOI] [PubMed] [Google Scholar]
  6. Djordjevic B., Evans R. G., Perez A. G., Weill M. K. Spontaneous unscheduled DNA synthesis in GI HeLa cells. Nature. 1969 Nov 22;224(5221):803–804. doi: 10.1038/224803a0. [DOI] [PubMed] [Google Scholar]
  7. Ensminger W. D., Tamm I. The step in cellular DNA synthesis blocked by Newcastle disease or mengovirus infection. Virology. 1970 Jan;40(1):152–165. doi: 10.1016/0042-6822(70)90387-9. [DOI] [PubMed] [Google Scholar]
  8. Evans R. G., Djordjevic B., Perez A. G. Temperature dependence of thymidine uptake and ability of thymidine incorporated during unscheduled DNA synthesis in irradiated and unirradiated HeLa cells. Int J Radiat Biol Relat Stud Phys Chem Med. 1970;18(3):277–280. doi: 10.1080/09553007014551101. [DOI] [PubMed] [Google Scholar]
  9. FRANKLIN R. M., BALTIMORE D. Patterns of macromolecular synthesis in normal and virus-infected mammalian cells. Cold Spring Harb Symp Quant Biol. 1962;27:175–198. doi: 10.1101/sqb.1962.027.001.019. [DOI] [PubMed] [Google Scholar]
  10. Fox M., Ayad S. R., Fox B. W. Characteristics of "repair synthesis" in x-irradiated P388F lymphoma cells. Int J Radiat Biol Relat Stud Phys Chem Med. 1970;18(2):101–110. doi: 10.1080/09553007014550911. [DOI] [PubMed] [Google Scholar]
  11. Hand R., Ensminger W. D., Tamm I. Cellular DNA replication in infections with cytocidal RNA viruses. Virology. 1971 Jun;44(3):527–536. doi: 10.1016/0042-6822(71)90366-7. [DOI] [PubMed] [Google Scholar]
  12. PETTIJOHN D., HANAWALT P. EVIDENCE FOR REPAIR-REPLICATION OF ULTRAVIOLET DAMAGED DNA IN BACTERIA. J Mol Biol. 1964 Aug;9:395–410. doi: 10.1016/s0022-2836(64)80216-3. [DOI] [PubMed] [Google Scholar]
  13. Painter R. B., Cleaver J. E. Repair replication, unscheduled DNA synthesis, and the repair of mammalian DNA. Radiat Res. 1969 Mar;37(3):451–466. [PubMed] [Google Scholar]
  14. Rasmussen R. E., Painter R. B. Radiation-stimulated DNA synthesis in cultured mammalian cells. J Cell Biol. 1966 Apr;29(1):11–19. doi: 10.1083/jcb.29.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. WHEELOCK E. F., TAMM I. Biochemical basis for alterations in structure and function of HeLa cells infected with Newcastle disease virus. J Exp Med. 1961 Nov 1;114:617–632. doi: 10.1084/jem.114.5.617. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES