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. 1971 Nov;8(5):649–655. doi: 10.1128/jvi.8.5.649-655.1971

Release of Envelope Glycoprotein from Rabies Virions by a Nonionic Detergent

Emese György 1,1, Mary C Sheehan 1, Frantisek Sokol 1
PMCID: PMC376242  PMID: 5167273

Abstract

Treatment of rabies virus with the nonionic detergent Nonidet P-40 resulted in solubilization of viral lipids and in a preferential release of the envelope glycoprotein. The other viral proteins and the viral ribonucleic acid remained associated in “core” particles sedimenting at a rate similar to that of intact virions. After fractionation of treated virus by velocity centrifugation in a sucrose density gradient, the amount of residual glycoprotein recovered in the “core” particle fraction and the extent of contamination of the glycoprotein fraction by other viral components were dependent on the ratio of detergent to viral protein used.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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