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Journal of Virology logoLink to Journal of Virology
. 1971 Nov;8(5):675–683. doi: 10.1128/jvi.8.5.675-683.1971

Intermediates in the Synthesis of Type 2 Adenovirus Deoxyribonucleic Acid

Marshall S Horwitz 1
PMCID: PMC376246  PMID: 5132696

Abstract

Intermediates in the synthesis of adenovirus type 2 deoxyribonucleic acid (DNA) were studied in HeLa cells. Pieces of DNA smaller than the viral genome were demonstrated after labeling with 3H-thymidine for 10 to 240 sec. Intermediates as small as the Okazaki fragments (8 to 10S) do not predominate at any of the above times. No detectable addition of nucleotides to parental genome could be shown, nor was there any breakdown of recently synthesized viral DNA. The DNA intermediates were of viral origin for they hybridized to viral DNA and were made at a stage of the cell cycle (G2) when host DNA is not synthesized.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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