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. 1971 Nov;8(5):690–694. doi: 10.1128/jvi.8.5.690-694.1971

Rescue of Murine Sarcoma Virus from a Sarcoma-Positive Leukemia-Negative Cell Line: Requirement for Replicating Leukemia Virus

P T Peebles 1, R H Bassin 1, D K Haapala 1, L A Phillips 1, S Nomura 1, P J Fischinger 1
PMCID: PMC376248  PMID: 4332138

Abstract

The nature of murine sarcoma virus (MSV) “defectiveness” was investigated by employing an MSV-transformed mouse 3T3 cell line which releases noninfectious virus-like particles. Rescue kinetics of MSV, observed after murine leukemia virus (MuLV) superinfection of these “sarcoma-positive leukemia-negative (S + L −)” mouse 3T3 cells, consisted of a 9− to 12-hr eclipse period followed by simultaneous release of both MSV and MuLV with no evidence for release of infectious MSV prior to the production of progeny MuLV. Addition of thymidine to the growth medium of MuLV-superinfected S + L − cells at a concentration suppressing deoxyribonucleic acid synthesis inhibited the replication of MuLV and the rescue of MSV. MSV production closely paralleled MuLV replication under a variety of experimental conditions. These results suggest that replication of MuLV is required for the rescue of infectious MSV from S + L − cells and that one (or more) factor, produced late in the MuLV replicative cycle, is utilized by both viruses during virion assembly. During the course of these experiments, virus stocks were recovered which contained infectious MSV in apparent excess over MuLV. These stocks were used for generating new S + L − cell lines by simple end point dilution procedures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A., Jainchill J. L., Todaro G. J. Murine sarcoma virus transformation of BALB-3T3 cells: lack of dependence on murine leukemia virus. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1236–1243. doi: 10.1073/pnas.66.4.1236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aaronson S. A., Rowe S. P. Nonproducer clones of murine sarcoma virus transformed BALB-3T3 cells. Virology. 1970 Sep;42(1):9–19. doi: 10.1016/0042-6822(70)90233-3. [DOI] [PubMed] [Google Scholar]
  3. Aaronson S. A., Todaro G. J. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. J Cell Physiol. 1968 Oct;72(2):141–148. doi: 10.1002/jcp.1040720208. [DOI] [PubMed] [Google Scholar]
  4. Bassin R. H., Phillips L. A., Kramer M. J., Haapala D. K., Peebles P. T., Nomura S., Fischinger P. J. Transformation of mouse 3T3 cells by murine sarcoma virus: release of virus-like particles in the absence of replicating murine leukemia helper virus. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1520–1524. doi: 10.1073/pnas.68.7.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bassin R. H., Tuttle N., Fischinger P. J. Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures. Int J Cancer. 1970 Jul 15;6(1):95–107. doi: 10.1002/ijc.2910060114. [DOI] [PubMed] [Google Scholar]
  6. Bassin R. H., Tuttle N., Fischinger P. J. Rapid cell culture assay technic for murine leukaemia viruses. Nature. 1971 Feb 19;229(5286):564–566. doi: 10.1038/229564b0. [DOI] [PubMed] [Google Scholar]
  7. Bather R., Leonard A. The role of DNA synthesis in virus replication and the morphological transformation of normal mouse embryo cells by MSV (Moloney). J Gen Virol. 1970 Jun;7(3):249–256. doi: 10.1099/0022-1317-7-3-249. [DOI] [PubMed] [Google Scholar]
  8. Duesberg P. H., Vogt P. K. On the role of DNA synthesis in avian tumor virus infection. Proc Natl Acad Sci U S A. 1969 Nov;64(3):939–946. doi: 10.1073/pnas.64.3.939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fischinger P. J., Moore C. O., O'Connor T. E. Isolation and identification of a helper virus found in the Moloney sarcoma-leukemia virus complex. J Natl Cancer Inst. 1969 Apr;42(4):605–622. [PubMed] [Google Scholar]
  10. Goldsby R. A., Zipser E. The isolation and replica plating of mammalian cell clones. Exp Cell Res. 1969 Feb;54(2):271–275. doi: 10.1016/0014-4827(69)90250-x. [DOI] [PubMed] [Google Scholar]
  11. HANAFUSA H., HANAFUSA T., RUBIN H. The defectiveness of Rous sarcoma virus. Proc Natl Acad Sci U S A. 1963 Apr;49:572–580. doi: 10.1073/pnas.49.4.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hanafusa H., Hanafusa T. Noninfectious RSV deficient in DNA polymerase. Virology. 1971 Jan;43(1):313–316. doi: 10.1016/0042-6822(71)90251-0. [DOI] [PubMed] [Google Scholar]
  13. Hanafusa H. Virus production by Rous sarcoma cells. Curr Top Microbiol Immunol. 1970;51:114–123. doi: 10.1007/978-3-642-46213-9_6. [DOI] [PubMed] [Google Scholar]
  14. Hartley J. W., Rowe W. P. Production of altered cell foci in tissue culture by defective Moloney sarcoma virus particles. Proc Natl Acad Sci U S A. 1966 Apr;55(4):780–786. doi: 10.1073/pnas.55.4.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hirschman S. Z., Fischinger P. J., O'Connor T. E. The inhibition by cytosine arabinoside of the replication of murine leukemia and sarcoma viruses in mouse embryo cultures. Int J Cancer. 1969 Sep 15;4(5):671–677. doi: 10.1002/ijc.2910040513. [DOI] [PubMed] [Google Scholar]
  16. Huebner R. J., Hartley J. W., Rowe W. P., Lane W. T., Capps W. I. Rescue of the defective genome of Moloney sarcoma virus from a noninfectious hamster tumor and the production of pseudotype sarcoma viruses with various murine leukemia viruses. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1164–1169. doi: 10.1073/pnas.56.4.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nakata Y., Bader J. P. Transformation by murine sarcoma virus: fixation (deoxyribonucleic acid synthesis) and development. J Virol. 1968 Nov;2(11):1255–1261. doi: 10.1128/jvi.2.11.1255-1261.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Robinson W. S., Robinson H. L. DNA polymerase in defective Rous sarcoma virus. Virology. 1971 May;44(2):457–462. doi: 10.1016/0042-6822(71)90278-9. [DOI] [PubMed] [Google Scholar]
  19. TODARO G. J., GREEN H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol. 1963 May;17:299–313. doi: 10.1083/jcb.17.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vigier P. RNA oncogenic viruses: structure, replication, and oncogenicity. Prog Med Virol. 1970;12:240–283. [PubMed] [Google Scholar]
  21. Wright B. S., O'Brien P. A., Shibley G. P., Mayyasi S. A., Lasfargues J. C. Infection of an established mouse bone marrow cell line (JLS-V9) with Rauscher and Moloney murine leukemia viruses. Cancer Res. 1967 Sep;27(9):1672–1677. [PubMed] [Google Scholar]
  22. Yoshikura H. Possible requirement of DNA synthesis for the growth of Friend leukemia virus. Jpn J Med Sci Biol. 1967 Jun;20(3):237–242. doi: 10.7883/yoken1952.20.237. [DOI] [PubMed] [Google Scholar]

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