Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1971 Nov;8(5):708–715. doi: 10.1128/jvi.8.5.708-715.1971

Integration of Simian Virus 40 Deoxyribonucleic Acid into the Deoxyribonucleic Acid of Primary Infected Chinese Hamster Cells

K Hirai 1, J Lehman 1,1, V Defendi 1,2
PMCID: PMC376251  PMID: 4332141

Abstract

Simian virus 40 deoxyribonucleic acid (DNA) became associated in an alkaline-stable form with the DNA of Chinese hamster embryo cells at 15 to 20 hr post-infection, at the time when cell DNA synthesis and T antigen were induced. The integration process was not inhibited by d-arabinosyl cytosine and was only partially inhibited by cycloheximide.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benjamin T. L., Burger M. M. Absence of a cell membrane alteration function in non-transforming mutants of polyoma virus. Proc Natl Acad Sci U S A. 1970 Oct;67(2):929–934. doi: 10.1073/pnas.67.2.929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burlingham B. T., Doerfler W. Three size-classes of intracellular adenovirus deoxyribonucleic acid. J Virol. 1971 Jun;7(6):707–719. doi: 10.1128/jvi.7.6.707-719.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cuzin F., Vogt M., Dieckmann M., Berg P. Induction of virus multiplication in 3T3 cells transformed by a thermosensitive mutant of polyoma virus. II. Formation of oligometric polyoma DNA molecules. J Mol Biol. 1970 Feb 14;47(3):317–333. doi: 10.1016/0022-2836(70)90305-0. [DOI] [PubMed] [Google Scholar]
  5. Doerfler W. Integration of the deoxyribonucleic acid of adenovirus type 12 into the deoxyribonucleic acid of baby hamster kidney cells. J Virol. 1970 Nov;6(5):652–666. doi: 10.1128/jvi.6.5.652-666.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dulbecco R., Eckhart W. Temperature-dependent properties of cells transformed by a thermosensitive mutant of polyoma virus. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1775–1781. doi: 10.1073/pnas.67.4.1775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hirai K., Defendi V. Homology between SV40 DNA and DNA of normal and SV40-transformed Chinese hamster cells. Biochem Biophys Res Commun. 1971 Feb 19;42(4):714–722. doi: 10.1016/0006-291x(71)90546-8. [DOI] [PubMed] [Google Scholar]
  8. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  9. IHLER G., MESELSON M. GENETIC RECOMBINATION IN BACTERIOPHAGE LAMBDA BY BREAKAGE AND JOINING OF DNA MOLECULES. Virology. 1963 Sep;21:7–10. doi: 10.1016/0042-6822(63)90297-6. [DOI] [PubMed] [Google Scholar]
  10. JENSEN F. C., GIRARDI A. J., GILDEN R. V., KOPROWSKI H. INFECTION OF HUMAN AND SIMIAN TISSUE CULTURES WITH ROUS SARCOMA VIRUS. Proc Natl Acad Sci U S A. 1964 Jul;52:53–59. doi: 10.1073/pnas.52.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KELLENBERGER G., ZICHICHI M. L., WEIGLE J. J. Exchange of DNA in the recombination of bacteriophage lambda. Proc Natl Acad Sci U S A. 1961 Jun 15;47:869–878. doi: 10.1073/pnas.47.6.869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lehman J. M., Defendi V. Changes in deoxyribonucleic acid synthesis regulation in Chinese hamster cells infected with simian virus 40. J Virol. 1970 Dec;6(6):738–749. doi: 10.1128/jvi.6.6.738-749.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MESELSON M., WEIGLE J. J. Chromosome brekage accompanying genetic recombination in bacteriophage. Proc Natl Acad Sci U S A. 1961 Jun 15;47:857–868. doi: 10.1073/pnas.47.6.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McConaughy B. L., McCarthy B. J. The interaction of oligodeoxynucleotides with denatured DNA. Biochim Biophys Acta. 1967 Nov 21;149(1):180–189. doi: 10.1016/0005-2787(67)90700-9. [DOI] [PubMed] [Google Scholar]
  15. Penman S. RNA metabolism in the HeLa cell nucleus. J Mol Biol. 1966 May;17(1):117–130. doi: 10.1016/s0022-2836(66)80098-0. [DOI] [PubMed] [Google Scholar]
  16. Sambrook J., Westphal H., Srinivasan P. R., Dulbecco R. The integrated state of viral DNA in SV40-transformed cells. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1288–1295. doi: 10.1073/pnas.60.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Weil J., Signer E. R. Recombination in bacteriophage lambda. II. Site-specific recombination promoted by the integration system. J Mol Biol. 1968 Jul 14;34(2):273–279. doi: 10.1016/0022-2836(68)90252-0. [DOI] [PubMed] [Google Scholar]
  18. Westphal H., Dulbecco R. Viral DNA in polyoma- and SV40-transformed cell lines. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1158–1165. doi: 10.1073/pnas.59.4.1158. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES