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. 1971 Nov;8(5):766–770. doi: 10.1128/jvi.8.5.766-770.1971

Structural Proteins of Adenovirus-Associated Viruses

J A Rose 1,2, J V Maizel Jr 1,2,1, J K Inman 1,2, A J Shatkin 1,2,2
PMCID: PMC376258  PMID: 5132697

Abstract

The structural proteins of adenovirus-associated virus (AAV) types 1, 2, and 3 were analyzed by acrylamide gel electrophoresis. In each case, one major protein (C) and two minor proteins (A and B) were identified. Component C had an estimated molecular weight of 62,000 daltons, and the molecular weights of components A and B were found to be 87,000 and 73,000 daltons, respectively. Coelectrophoresis of adenovirus and AAV proteins revealed an overlap only between the adenovirus fiber-penton component and the AAV C polypeptide. Among AAV serotypes, homologous components were electrophoretically identical, except that the C component of AAV-2 was of slightly lower molecular weight than the C components of AAV-1 and AAV-3. The relative incorporation of 14C-arginine and 14C-mixed amino acids into the three polypeptides of AAV-2 was similar, indicating an absence of an arginine-rich component. In addition, AAV-2 was found to have a substantially lower arginine content than helper adenoviruses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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