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. 1971 Nov;8(5):771–777. doi: 10.1128/jvi.8.5.771-777.1971

Adenovirus-Associated Virus Multiplication

VI. Base Composition of the Deoxyribonucleic Acid Strand Species and Strand-Specific In Vivo Transcription

J A Rose 1, F Koczot 1
PMCID: PMC376259  PMID: 5132698

Abstract

The two complementary strand species of 5-bromodeoxyuridine-substituted, adenovirus-associated virus type 2 (AAV-2) deoxyribonucleic acid were preparatively separated in CsCl density gradients and further purified by sedimentation through 5 to 20% sucrose. The base composition of each strand species was determined, and it was found that the species banding at a greater density in CsCl (heavy strands) had an expected higher thymidine content (26.5%) than that 21.7%) of the less dense species (light strands). Furthermore, the base composition of in vivo-synthesized, AAV-specific ribonucleic acid was similar to that of light-strand deoxyribonucleic acid, and this ribonucleic acid apparently hybridized only with heavy strands. These observations indicate that the heavy-strand species alone serves as the transcriptional template in vivo. This study represents the first instance in which the base composition and specificity of in vivo transcription have been determined for each of the complementary strands of an animal virus deoxyribonucleic acid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ATCHISON R. W., CASTO B. C., HAMMON W. M. ADENOVIRUS-ASSOCIATED DEFECTIVE VIRUS PARTICLES. Science. 1965 Aug 13;149(3685):754–756. doi: 10.1126/science.149.3685.754. [DOI] [PubMed] [Google Scholar]
  2. Aloni Y., Winocour E., Sachs L. Characterization of the simian virus 40-specific RNA in virus-yielding and transformed cells. J Mol Biol. 1968 Feb 14;31(3):415–429. doi: 10.1016/0022-2836(68)90418-x. [DOI] [PubMed] [Google Scholar]
  3. BAUTZ E. K., HALL B. D. The isolation of T4-specific RNA on a DNA-cellulose column. Proc Natl Acad Sci U S A. 1962 Mar 15;48:400–408. doi: 10.1073/pnas.48.3.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berns K. I., Rose J. A. Evidence for a single-stranded adenovirus-associated virus genome: isolation and separation of complementary single strands. J Virol. 1970 Jun;5(6):693–699. doi: 10.1128/jvi.5.6.693-699.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Colvill A. J., Kanner L. C., Tocchini-Valentini G. P., Sarnat M. T., Geiduschek E. P. Asymmetric RNA synthesis in vitro: heterologous DNA-enzyme systems; E. coli RNA polymerase. Proc Natl Acad Sci U S A. 1965 May;53(5):1140–1147. doi: 10.1073/pnas.53.5.1140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Crawford L. V. A minute virus of mice. Virology. 1966 Aug;29(4):605–612. doi: 10.1016/0042-6822(66)90284-4. [DOI] [PubMed] [Google Scholar]
  7. Crawford L. V., Follett E. A., Burdon M. G., McGeoch D. J. The DNA of a minute virus of mice. J Gen Virol. 1969 Jan;4(1):37–46. doi: 10.1099/0022-1317-4-1-37. [DOI] [PubMed] [Google Scholar]
  8. GREEN M., PINA M. Biochemical studies on adenovirus multiplication. IV. Isolation, purification, and chemical analysis of adenovirus. Virology. 1963 May;20:199–207. doi: 10.1016/0042-6822(63)90157-0. [DOI] [PubMed] [Google Scholar]
  9. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  10. HAYASHI M., HAYASHI M. N., SPIEGELMAN S. RESTRICTION OF IN VIVO GENETIC TRANSCRIPTION TO ONE OF THE COMPLEMENTARY STRANDS OF DNA. Proc Natl Acad Sci U S A. 1963 Oct;50:664–672. doi: 10.1073/pnas.50.4.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hoggan M. D., Blacklow N. R., Rowe W. P. Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1467–1474. doi: 10.1073/pnas.55.6.1467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hoggan M. D., Shatkin A. J., Blacklow N. R., Koczot F., Rose J. A. Helper-dependent infectious deoxyribonucleic acid from adenovirus-associated virus. J Virol. 1968 Aug;2(8):850–851. doi: 10.1128/jvi.2.8.850-851.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mayor H. D., Torikai K., Melnick J. L., Mandel M. Plus and minus single-stranded DNA separately encapsidated in adeno-associated satellite virions. Science. 1969 Dec 5;166(3910):1280–1282. doi: 10.1126/science.166.3910.1280. [DOI] [PubMed] [Google Scholar]
  14. Parks W. P., Green M., Piña M., Melnick J. L. Physicochemical characterization of adeno-associated satellite virus type 4 and its nucleic acid. J Virol. 1967 Oct;1(5):980–987. doi: 10.1128/jvi.1.5.980-987.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rose J. A., Berns K. I., Hoggan M. D., Koczot F. J. Evidence for a single-stranded adenovirus-associated virus genome: formation of a DNA density hybrid on release of viral DNA. Proc Natl Acad Sci U S A. 1969 Nov;64(3):863–869. doi: 10.1073/pnas.64.3.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rose J. A., Hoggan M. D., Koczot F., Shatkin A. J. Genetic relatedness studies with adenovirus-associated viruses. J Virol. 1968 Oct;2(10):999–1005. doi: 10.1128/jvi.2.10.999-1005.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rose J. A., Hoggan M. D., Shatkin A. J. Nucleic acid from an adeno-associated virus: chemical and physical studies. Proc Natl Acad Sci U S A. 1966 Jul;56(1):86–92. doi: 10.1073/pnas.56.1.86. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rose J. A., Reich P. R., Weissman S. M. RNA production in adenovirus-infected KB cells. Virology. 1965 Dec;27(4):571–579. doi: 10.1016/0042-6822(65)90183-2. [DOI] [PubMed] [Google Scholar]
  19. SCHERRER K., DARNELL J. E. Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochem Biophys Res Commun. 1962 Jun 4;7:486–490. doi: 10.1016/0006-291x(62)90341-8. [DOI] [PubMed] [Google Scholar]
  20. SEBRING E. D., SALZMAN N. P. AN IMPROVED PROCEDURE FOR MEASURING THE DISTRIBUTION OF P32O4--AMONG THE NUCLEOTIDES OF RIBONUCLEIC ACID. Anal Biochem. 1964 May;8:126–129. doi: 10.1016/0003-2697(64)90177-0. [DOI] [PubMed] [Google Scholar]
  21. SINSHEIMER R. L., STARMAN B., NAGLER C., GUTHRIE S. The process of infection with bacteriophage phi-XI74. I. Evidence for a "replicative form". J Mol Biol. 1962 Mar;4:142–160. doi: 10.1016/s0022-2836(62)80047-3. [DOI] [PubMed] [Google Scholar]
  22. Salzman L. A., Jori L. A. Characterization of the Kilham rat virus. J Virol. 1970 Feb;5(2):114–122. doi: 10.1128/jvi.5.2.114-122.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Salzman L. A., White W. L., Kakefuda T. Linear, single-stranded deoxyribonucleic acid isolated from Kilham rat virus. J Virol. 1971 Jun;7(6):830–835. doi: 10.1128/jvi.7.6.830-835.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Strauss J. H., Jr, Kelly R. B., Sinsheimer R. L. Denaturation of RNA with dimethyl sulfoxide. Biopolymers. 1968 Jun;6(6):793–807. doi: 10.1002/bip.1968.360060604. [DOI] [PubMed] [Google Scholar]
  25. Vernon S. K., Stasny J. T., Neurath A. R., Rubin B. A. Electron microscopy of DNA from adeno-associated virus type I. J Gen Virol. 1971 Mar;10(3):267–272. doi: 10.1099/0022-1317-10-3-267. [DOI] [PubMed] [Google Scholar]
  26. Vogt V. Breaks in DNA stimulate transcription by core RNA polymerase. Nature. 1969 Aug 23;223(5208):854–855. doi: 10.1038/223854a0. [DOI] [PubMed] [Google Scholar]

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