Table 1. Upregulated transcripts in three mouse models of SPEM.
Normal chief cells and SPEM lineages from 3 SPEM models were compared by gene microarray (6 mice per group). Five comparison categories were established: Pan-SPEM (I), Acute SPEM (II), SPEM with Inflammation (III), Specific to SPEM with Inflammation (IV), and SPEM with Chronic Inflammation (V). The linear fold change in each sample group is listed as compared to the column marked with a bullet point (•). In Categories I, II, and III, fold changes are upregulated as compared to Chief Cells. Category IV changes are compared to DMP-777. In Category V, listed transcripts are upregulated in H. felis as compared to each of the other groups; thus fold changes are shown as relative downregulation in the respective groups. Asterisks denote transcripts previously found upregulated in human metaplasias of the stomach.[11]
| I. Pan-SPEM | ||||
|---|---|---|---|---|
| Chief Cells | DMP-777 | L635 | H.felis | |
|
|
||||
| *Ccnb2 | ● | 2.01 | 2.55 | 1.82 |
| *Cenpk | ● | 1.35 | 2.60 | 1.27 |
| Clu | ● | 1.04 | 1.58 | 1.38 |
| Cxcl17 | ● | 1.50 | 1.81 | 1.56 |
| Slfn9 | ● | 1.33 | 2.18 | 1.33 |
| *Top2a | ● | 1.95 | 2.92 | 2.11 |
| Traf4 | ● | 1.75 | 2.18 | 1.59 |
| Wfdc2 | ● | 1.62 | 1.50 | 3.12 |
| II. Acute SPEM | ||||
| Chief Cells | DMP-777 | L635 | ||
|
|
||||
| Chek2 | ● | 1.23 | 1.42 | |
| Fignl1 | ● | 1.19 | 1.93 | |
| Mmp12 | ● | 1.91 | 2.79 | |
| Tmem48 | ● | 0.78 | 1.76 | |
| Ube2c | ● | 1.61 | 2.88 | |
|
| ||||
| III. SPEM with Inflammation | ||||
| Chief Cells | L635 | H.felis | ||
|
|
|
|||
| *Cftr | ● | 3.14 | 5.78 | |
| *Ctss | ● | 1.24 | 2.03 | |
| *Dmbt1 | ● | 2.72 | 2.77 | |
| Etv5 | ● | 2.01 | 3.13 | |
| *Gpx2 | ● | 2.36 | 3.00 | |
| *Mad2l1 | ● | 2.12 | 1.78 | |
| *Prom1 | ● | 0.73 | 1.44 | |
| IV. Specific to SPEM with Inflammation | ||||
| DMP-777 | L635 | H.felis | ||
|
|
||||
| Arhgap9 | ● | 0.84 | 0.77 | |
| Cd14 | ● | 1.53 | 2.72 | |
| Ceacam10 | ● | 0.36 | 2.17 | |
| Glipr1 | ● | 0.98 | 0.84 | |
| Gpr171 | ● | 1.89 | 2.17 | |
| Ly6a | ● | 0.75 | 1.26 | |
| Lyz2 | ● | 2.53 | 1.97 | |
| Ms4a6b | ● | 1.45 | 1.94 | |
| Ms4a6c | ● | 2.13 | 2.03 | |
| Tyrobp | ● | 1.47 | 1.15 | |
| V. SPEM with Chronic Inflammation | ||||
| Chief Cells | DMP-777 | L635 | H.felis | |
|
|
||||
| *Casp1 | −4.10 | −4.45 | −4.32 | ● |
| *Ceacam1 | −3.44 | −3.05 | −2.28 | ● |
| *Gpa33 | −2.93 | −2.74 | −3.60 | ● |
| *Il18r1 | −3.09 | −1.66 | −1.99 | ● |
| *Itga2 | −2.87 | −2.61 | −2.81 | ● |
| *Muc4 | −3.74 | −3.34 | −3.77 | ● |
| *Pigr | −5.37 | −3.29 | −3.16 | ● |
| *Vil1 | −2.59 | −2.26 | −2.60 | ● |