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Journal of Virology logoLink to Journal of Virology
. 1971 Dec;8(6):836–841. doi: 10.1128/jvi.8.6.836-841.1971

Isolation and Characterization of Temperature-Sensitive Mutants of Vesicular Stomatitis Virus, New Jersey Serotype

C R Pringle 1, I B Duncan 1, Mary Stevenson 1
PMCID: PMC376272  PMID: 4366429

Abstract

Forty-eight temperature-sensitive (ts) mutants have been isolated from a wild-type strain of the New Jersey serotype of vesicular stomatitis virus (VSV) after exposure to the base analogue mutagen 5-fluorouracil. Of these mutants, 47 have been classified into 6 nonoverlapping complementation groups containing 21, 17, 4, 3, 2, and 1 mutant, respectively (1 mutant remaining unallocated). The ribonucleic acid (RNA) phenotype of 23 of these mutants has been established. Four of the six groups contain one or more mutants unable to synthesize detectable amounts of viral RNA under restrictive conditions (39 C). No complementation was observed in mixed infection with ts mutants from the five established complementation groups of the Indiana serotype of VSV.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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