Abstract
One hundred and fifty-nine prompt lactose-fermenting strains of Enterobacteriaceae were tested for H2S production, ornithine decarboxylase activity, motility, and citrate utilization by using two different sets of test media. It was shown that the three tests first mentioned could be carried out in a single tube, the results being as reliable as when each was done separately. Simmons medium was preferred for the detection of citrate utilization. Since these four tests require only two tubes, they are considered more convenient in routine diagnostics. A combined medium for the detection of H2S production and motility is described.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- GERSHMAN M. MODIFIED MOTILITY-SULFIDE MEDIUM. J Bacteriol. 1963 Nov;86:1122–1123. doi: 10.1128/jb.86.5.1122-1123.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnson J. G., Kunz L. J., Barron W., Ewing W. H. Biochemical differentiation of the Enterobacteriaceae with the aid of lysine-iron-agar. Appl Microbiol. 1966 Mar;14(2):212–217. doi: 10.1128/am.14.2.212-217.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steel K. J. Microbial identification. J Gen Microbiol. 1965 Jul;40(1):143–148. doi: 10.1099/00221287-40-1-143. [DOI] [PubMed] [Google Scholar]
- Wolfe M. W., Amsterdam D. New diagnostic system for the identification of lactose-fermenting gram-negative rods. Appl Microbiol. 1968 Oct;16(10):1528–1531. doi: 10.1128/am.16.10.1528-1531.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]