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. 1971 Sep;22(3):387–392. doi: 10.1128/am.22.3.387-392.1971

l-Asparaginase from Proteus vulgaris1

Tetsuya Tosa 1,2, Ryujiro Sano 1,2, Kozo Yamamoto 1,2, Masatoshi Nakamura 1,2, Katsuko Ando 1,2, Ichiro Chibata 1,2
PMCID: PMC376319  PMID: 5000866

Abstract

To produce an immunologically and enzymologically new type of l-asparaginase, 108 strains of bacteria were screened for enzyme production. As a result, 13 bacteria belonging to the genera Alcaligenes, Bacterium, and Proteus were found to produce l-asparaginases in high levels. Among these l-asparaginases, partially purified l-asparaginases from B. cadaveris and P. vulgaris showed antitumor activity. A partially purified l-asparaginase preparation of P. vulgaris did not react with the antibody of Escherichia colil-asparaginase on the Ouchterlony agar plate. Culture conditions for the production of l-asparaginase by P. vulgaris were investigated in detail. The enzyme was produced in high yields when cells were grown aerobically in a medium containing sodium fumarate and corn steep liquor. The addition of glucose or ammonium ion to the medium, however, resulted in depressed production of l-asparaginase. Under the optimum conditions, 3,700 international units of l-asparaginase was obtained from 1 liter of culture medium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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