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. 1971 Sep;22(3):393–396. doi: 10.1128/am.22.3.393-396.1971

High Aflatoxin Production on a Chemically Defined Medium 1

T V Reddy 1, L Viswanathan 1, T A Venkitasubramanian 1
PMCID: PMC376320  PMID: 5119206

Abstract

Aspergillus parasiticus ATCC 15517 produced 28 to 30 mg of aflatoxin per 100 ml of a medium containing sucrose, asparagine, and salts in stationary and shaken cultures. In the absence of asparagine in the medium, the toxin yields fell drastically, and the thin-layer chromatograms of the chloroform extracts of the cultures indicated the total absence of aflatoxin G1 and the presence of new intense blue and green fluorescent bands having RF values lower than aflatoxins. Initial pH was critical and had to be around 4.5 for good growth and high toxin production on this medium. Optimum concentrations of KH2PO4 and MgSO4·7H2O in the medium were much lower than those normally used in fungal growth media.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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