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. 1971 Sep;22(3):422–427. doi: 10.1128/am.22.3.422-427.1971

Reduction of Nucleic Acid Content in Candida Yeast Cells by Bovine Pancreatic Ribonuclease A Treatment

A C Castro 1, A J Sinskey 1, S R Tannenbaum 1
PMCID: PMC376325  PMID: 5165838

Abstract

Yeast as a source of protein for human consumption is limited by its relatively high nucleic acid content. In this study, we developed an enzymatic method of decreasing the nucleic acid content. Candida utilis cells, heat-shocked at 80 C for 30 sec, were treated with bovine pancreatic ribonuclease A. Maximum leakage of nucleic acid was observed when the incubation temperature was between 55 and 65 C, the pH of the system from 6.75 to 8.0, and the enzyme-to-cell ratio 1:10,000 on a weight-by-weight basis. Other factors, such as yeast strain, age of cells, and method of propagation, did not influence the susceptibility of the yeast cells to the action of ribonuclease. Buffers and monovalent cations had no inhibiting effects. Magnesium and calcium ions at concentrations greater than 0.001 m showed marked inhibition on the rate of nucleic acid leakage. This enzymatic method reduced the nucleic acid content of yeast cells from 7.5 to 9.0% to 1.5 to 2.0% with no significant concomitant loss of protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allwood M. C., Russell A. D. Thermally induced ribonucleic acid degradation and leakage of substances from the metabolic pool in Staphylococcus aureus. J Bacteriol. 1968 Feb;95(2):345–349. doi: 10.1128/jb.95.2.345-349.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Edozien J. C., Udo U. U., Young V. R., Scrimshaw N. S. Effects of high levels of yeast feeding on uric acid metabolism of young man. Nature. 1970 Oct 10;228(5267):180–180. doi: 10.1038/228180a0. [DOI] [PubMed] [Google Scholar]
  3. Eichhorn G. L., Clark P., Tarien E. The interaction of metal ions with polynucleotides and related compounds. 13. The effect of metal ions on the enzymatic degradation of ribonucleic acid by bovine pancreatic ribonuclease and of deoxyribonucleic acid by bovine pancreatic deoxyribonuclease I. J Biol Chem. 1969 Feb 10;244(3):937–942. [PubMed] [Google Scholar]
  4. Haight R. D., Ordal Z. J. Thermally induced degradation of staphylococcal ribosomes. Can J Microbiol. 1969 Jan;15(1):15–19. doi: 10.1139/m69-003. [DOI] [PubMed] [Google Scholar]
  5. Iandolo J. J., Ordal Z. J. Repair of thermal injury of Staphylococcus aureus. J Bacteriol. 1966 Jan;91(1):134–142. doi: 10.1128/jb.91.1.134-142.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Maul S. B., Sinskey A. J., Tannenbaum S. R. New process for reducing the nucleic acid content of yeast. Nature. 1970 Oct 10;228(5267):181–181. doi: 10.1038/228181a0. [DOI] [PubMed] [Google Scholar]
  7. Morrill G. A., Reiss M. M. Inhibition of enzymatic degradation of RNA by bound calcium and magnesium. Biochim Biophys Acta. 1969 Mar 18;179(1):43–49. doi: 10.1016/0005-2787(69)90120-8. [DOI] [PubMed] [Google Scholar]
  8. NECAS O. Action of ribonuclease on yeast protoplasts. Exp Cell Res. 1958 Feb;14(1):216–219. doi: 10.1016/0014-4827(58)90232-5. [DOI] [PubMed] [Google Scholar]
  9. Ohta S., Maul S., Sinskey A. J., Tannenbaum S. R. Characterization of a heat-shock process for reduction of the nucleic acid content of Candida utilis. Appl Microbiol. 1971 Sep;22(3):415–421. doi: 10.1128/am.22.3.415-421.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SCHLENK F., DAINKO J. L. ACTION OF RIBONUCLEASE PREPARATIONS ON VIABLE YEAST CELLS AND SPHEROPLASTS. J Bacteriol. 1965 Feb;89:428–436. doi: 10.1128/jb.89.2.428-436.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. STICKLAND L. H. The determination of small quantities of bacteria by means of the biuret reaction. J Gen Microbiol. 1951 Oct;5(4):698–703. doi: 10.1099/00221287-5-4-698. [DOI] [PubMed] [Google Scholar]
  12. de KLOET S., van WERMESKERKEN R., KONINGSBERGER V. V. Studies on protein synthesis by protoplasts of Saccharomyces carlsbergensis. I. The effect of ribonuclease on protein synthesis. Biochim Biophys Acta. 1961 Feb 12;47:138–143. doi: 10.1016/0006-3002(61)90838-1. [DOI] [PubMed] [Google Scholar]

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