Table 1.
Persistence of antibodies, measured by ELISA and SNT, in the milk and blood of adult dairy cattle four years post-vaccination with a BTV-8 inactivated vaccine.
| Cow number | Milk ELISA (%S/P)a |
Early detection ELISA (%S/P)b |
SNT log10 |
|||
|---|---|---|---|---|---|---|
| 2011 | 2012 | 2011 | 2012 | 2011 | 2012 | |
| 1 | Pos (290) | Pos (153) | Pos (184) | Pos (254) | Pos (1.18) | Pos (1.48) |
| 2 | Pos (221) | Neg (79) | Pos (184) | Pos (251) | Pos (1.48) | Pos (1.00) |
| 3 | Pos (262) | Pos (142) | Pos (165) | Pos (267) | Pos (1.78) | Pos (1.78) |
| 4 | Neg (68) | Neg (24) | Pos (178) | Pos (224) | Pos (1.60) | Pos (1.48) |
| 5 | Pos (307) | No sample | Pos (193) | Pos (280) | Pos (1.00) | Pos (1.78) |
| 6 | Pos (305) | Pos (144) | Pos (190) | Pos (255) | Pos (2.38) | Pos (2.20) |
| 7 | Pos (206) | Pos (134) | Pos (193) | Pos (267) | Pos (1.60) | Pos (2.20) |
| 8 | Pos (140) | Pos (113) | Pos (193) | Pos (280) | Pos (2.20) | Pos (1.78) |
| 9 | Pos (301) | Pos (145) | Pos (183) | Pos (262) | Pos (1.78) | Pos (1.78) |
| 10 | Pos (295) | Pos (146) | Pos (192) | Pos (255) | Pos (2.08) | Pos (2.38) |
| 11 | Pos (292) | Pos (146) | Pos (122) | Pos (185) | Pos (1.60) | Pos (1.90) |
| 12 | Pos (189) | Pos (125) | Pos (153) | Pos (230) | Pos (1.18) | Pos (1.60) |
| 13 | Pos (281) | Pos (131) | Pos (193) | Pos (280) | Pos (1.48) | Pos (1.18) |
| 14 | Pos (286) | Pos (139) | Pos (170) | Pos (240) | Pos (1.18) | Pos (1.60) |
| 15 | Pos (229) | Pos (125) | Pos (183) | Pos (264) | Pos (1.18) | Pos (1.00) |
| 16 | Pos (298) | Pos (152) | Pos (187) | Pos (266) | Pos (1.78) | Pos (2.08) |
| 17 | Pos (304) | Pos (148) | Pos (193) | Pos (266) | Pos (1.30) | Pos (1.78) |
| 18 | Pos (300) | Pos (137) | Pos (185) | Pos (268) | Pos (2.51) | Pos (1.90) |
| 19 | Inc (98) | Neg (68) | Pos (165) | Pos (198) | Pos (1.00) | Pos (1.18) |
| 20 | Pos (241) | Pos (141) | Pos (193) | Pos (276) | Pos (1.30) | Pos (1.48) |
| 21 | Pos (247) | Pos (124) | Pos (191) | Pos (262) | Pos (1.48) | Pos (1.18) |
| 22 | Pos (260) | Pos (114) | Pos (193) | Pos (240) | Pos (1.60) | Pos (1.60) |
| 23 | Pos (306) | Pos (150) | Pos (193) | Pos (270) | Pos (1.78) | Pos (1.78) |
| 24 | Pos (306) | Pos (153) | Pos (189) | Pos (268) | Pos (1.00) | Pos (1.18) |
| 25 | Pos (301) | Pos (151) | Pos (187) | Pos (269) | Pos (1.48) | Pos (1.60) |
| 26 | Neg (7) | Neg (-8) | Neg (22) | Neg (18) | Neg | Neg |
| 27 | Neg (52) | Pos (135) | Pos (176) | Pos (263) | Pos (1.18) | Pos (1.18) |
| 28 | Neg (50) | Neg (58) | Pos (107) | Pos (99) | Pos (1.30) | Pos (1.78) |
| 29 | Pos (268) | Pos (137) | Pos (181) | Pos (248) | Pos (1.60) | Pos (1.60) |
SNT: serum neutralisation test; Pos: positive; Neg: negative; Inc: inconclusive.
% S/P: percentage of positivity compared to the positive control.
Bold value shows the negative results.
a
Individual samples: negative ≤90%, inconclusive 90–110%, positive ≥110%.
b
Negative ≤25%, inconclusive 25–30%, positive ≥30%.