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. 1971 Oct;22(4):671–677. doi: 10.1128/am.22.4.671-677.1971

Dye Uptake Assay: an Efficient and Sensitive Method for Human Interferon Titration

Anne L R Pidot 1
PMCID: PMC376384  PMID: 4331773

Abstract

Currently, human interferon (IF) assays are generally performed by plaque reduction or visual cytopathic effect methods. Both are time-consuming, subjective in interpretation, and, in the case of the latter, relatively insensitive. An adaption of the dye uptake method for human IF titration which uses foreskin-derived fibroblasts and vesicular stomatitis virus is described. This assay is reproducible and sensitive (1 unit = 3 international units). By direct comparison, however, it is somewhat less sensitive than the plaque reduction assay (1 unit = 1.1 international units). This assay is especially recommended for use in needed clinical investigations of human IF because of its technical simplicity, allowing efficient handling of large numbers of specimens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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