Skip to main content
PMC home page
Applied Microbiology logoLink to Applied Microbiology
. 1971 Oct;22(4):688–692. doi: 10.1128/am.22.4.688-692.1971

Improved Medium for Enumeration of Clostridium perfringens

Stanley M Harmon 1, Donald A Kautter 1, James T Peeler 1
PMCID: PMC376387  PMID: 4331774

Abstract

An improved selective medium, Tryptose-sulfite-cycloserine (TSC) agar, for the enumeration of Clostridium perfringens is described. It consists of the same basal medium as Shahidi-Ferguson-perfringens (SFP) agar, but with 400 μg of D-cycloserine per ml substituted for the kanamycin and polymyxin. Tolerance of C. perfringens for D-cycloserine, its production of lecithinase, and its ability to reduce sulfite were used as the basis for development of this medium. Comparisons were made between TSC and SFP agars for the recovery of vegetative cells of C. perfringens by using statistical methods. The results showed that TSC allowed virtually complete recovery of most of the C. perfringens strains while inhibiting practically all facultative anaerobes tested. SFP agar allowed a slightly higher rate of recovery of C. perfringens but was found to be much less selective.

Full text

PDF
688

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANGELOTTI R., HALL H. E., FOTER M. J., LEWIS K. H. Quantitation of Clostridium perfringens in foods. Appl Microbiol. 1962 May;10:193–199. doi: 10.1128/am.10.3.193-199.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Füzi M., Csukás Z. New selective medium for the isolation of Clostridium perfringens. Acta Microbiol Acad Sci Hung. 1969;16(3):273–278. [PubMed] [Google Scholar]
  3. HOBBS B. C., SMITH M. E., OAKLEY C. L., WARRACK G. H., CRUICKSHANK J. C. Clostridium welchii food poisoning. J Hyg (Lond) 1953 Mar;51(1):75–101. doi: 10.1017/s0022172400015515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Harmon S. M., Kautter D. A., Peeler J. T. Comparison of media for the enumeration of Clostridium perfringens. Appl Microbiol. 1971 May;21(5):922–927. doi: 10.1128/am.21.5.922-927.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MARSHALL R. S., STEENBERGEN J. F., MCCLUNG L. S. RAPID TECHNIQUE FOR THE ENUMERATION OF CLOSTRIDIUM PERFINGENS. Appl Microbiol. 1965 Jul;13:559–563. doi: 10.1128/am.13.4.559-563.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. McClung L. S., Toabe R. The Egg Yolk Plate Reaction for the Presumptive Diagnosis of Clostridium sporogenes and Certain Species of the Gangrene and Botulinum Groups. J Bacteriol. 1947 Feb;53(2):139–147. doi: 10.1128/jb.53.2.139-147.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Shahidi S. A., Ferguson A. R. New quantitative, qualitative, and confirmatory media for rapid analysis of food for Clostridium perfringens. Appl Microbiol. 1971 Mar;21(3):500–506. doi: 10.1128/am.21.3.500-506.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Solomon H. M., Lynt R. K., Jr, Kautter D. A., Lilly T., Jr Serological studies of Clostridium botulinum type E and related organisms. II. Serology of spores. J Bacteriol. 1969 May;98(2):407–414. doi: 10.1128/jb.98.2.407-414.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES