Effects of Light Intensity and Nitrogen Starvation on Glycerolipid, Glycerophospholipid, and Carotenoid Composition in Dunaliella tertiolecta Culture

Abstract

Time-course variation of lipid and carotenoid production under high light (300 μE/m²s) and nitrogen starvation conditions was determined in a Dunaliella tertiolecta strain. Nanoelectrospray (nanoESI) chip based direct infusion was used for lipid analysis and ultra-performance liquid chromatography (UPLC) coupled with a photodiode array (PDA) or atmospheric chemical ionization mass spectrometry (APCI-MS) was used for carotenoid analysis. A total of 29 lipids and 7 carotenoids were detected. Alterations to diacylglyceryltrimethylhomoserine (DGTS) and digalactosyldiacylglycerol (DGDG) species were significant observations under stress conditions. Their role in relation to the regulation of photosynthesis under stress condition is discussed in this study. The total carotenoid content was decreased under stress conditions, while ã-carotene was increased under nitrate-deficient cultivation. The highest productivity of carotenoid was attained under high light and nitrate sufficiency (HLNS) condition, which result from the highest level of biomass under HLNS. When stress was induced at stationary phase, the substantial changes to the lipid composition occurred, and the higher carotenoid content and productivity were exhibited. This is the first report to investigate the variation of lipids, including glycerolipid, glycerophospholipid, and carotenoid in D. tertiolecta in response to stress conditions using lipidomics tools.

Introduction

Dunaliella tertiolecta was on the focus of this study because of its relatively high oil content, rapid growth rate, and high tolerance to extreme conditions such as high salinity [1]–[3]. It facilitates the extraction process because of its lack of a rigid cell wall, making it a promising resource for producing biofuel and pharmaceutical materials such as carotenoids [4]. Until now, most of studies on D. tertiolecta have only focused on analyzing content and composition of fatty acids and measuring cell growth or total lipid content [5], [6]. However, there were no reports of individual lipid species and carotenoid profiling in D. tertiolecta cultures. Moreover, only a few of reports of lipidomics studies based on mass spectrometry on other microalgae species have been published to date [7]–[9].

Although traditional analytical technologies, particularly thin-layer chromatography (TLC), have been used as the major tools to investigate lipids in microalgae [10], [11], after developing direct-infusion electrospray ionization-mass spectrometry (ESI-MS), it has been adopted by investigators to identify algal lipidome because of its relatively simple analytical process and potential for high-throughput analysis [12].

To survive in extreme environments, microalgae contain characteristic lipid species such as betaine lipids and alter the chain length and/or the degree of saturation. In other words, they induce specific alterations in lipids to resist against stress conditions [13]. However, lipidomics research regarding the pathways and alterations of individual lipid species in microalgae under various stress condition has been lacking until now, despite the importance of elucidating the biological role of lipids as a defense response to stress conditions.

The hypothesis of this study is that considerable alterations to lipids species and their compositions would occur under nitrogen-starvation or high light intensity conditions in D. tertiolecta cells. The objectives of this study are to identify individual lipid species and carotenoid, and investigate the change tendency of lipids over the stress time point and duration under various stress condition. The carotenoid content and productivity under several different stress conditions were also determined.

Materials and Methods

Chemicals and Reagents

HPLC-grade acetonitrile, methanol, chloroform, water and hexane were purchased from Honeywell Burdick & Jackson (Muskegon, MI, USA). HPLC-grade methyl tertiary butyl ether (MTBE), butylated hydroxyl toluene (BHT), β-apo-8′-carotenal, and ammonium acetate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Carotenoid (neoxanthin, NEO; violaxanthin, VIO; anthraxanthin, ANT; zeaxanthin, ZEA; lutein, LUT; β-cryptoxanthin, βCRYP; lycopene, LYC; neurosporene, NEU; γ-carotene, γCAR; α-carotene, αCAR; β-carotene, βCAR;) standards were obtained from Carotenature (Lupsingen, Switzerland). LC-MS grade formic acid was purchased from Fisher Scientific (Loughborough, UK). Phospholipids (phosphatidylcholine, PC; phosphatidylglycerol, PG; phosphatidylinositol, PI; phosphatidylethanolamine, PE; lysophosphatidylcholine, LPC; lysophosphatidylinositol, LPI) and two glycolipids (monogalactosyldiacylglycerol, MGDG; digalactosyldiacylglycerol, DGDG) standards used in this study were purchased from Avanti Polar Lipids (Alabaster, AL, USA). A standard of sulfoquinovosyldiacylglycerol (SQDG) was obtained from Lipid Products (Redhill, UK). All chemicals were of analytical grade or higher purity.

Strain Culture

The green microalga D. tertiolecta (UTEX LB999) was obtained from the Culture Collection of Algae at the University of Texas at Austin (UTEX). Cells were grown photoautotrophically in three folded f/2 medium [14] except vitamin solution at 21°C and irradiated with fluorescent lamps at 50 μE/m²s. The optimal light intensity was determined in a preliminary experiment, as described previously [15]. A constant nitrate concentration of 2.68 mM was maintained by supplying NO₃ stock solution every 24 hours after measuring the NO₃ concentration. D. tertiolecta cultures were set up in 400 mL bubble-column photobioreactors [16] at a 0.1 g/L fresh cell weight (FCW) inoculation concentration and a flow rate of 0.1 vvm bubbled air that contained 2% CO₂.

Stress Conditions

A stress was triggered in middle of the exponential phase and the beginning of the stationary phase. Based on a preliminary study, each point was determined by the fresh cell weight and cell concentration, which were 1.7 g/L (4.3×10⁷ cells/L) and 3.2 g/L (1.3×10⁷ cells/L), respectively. The cells were harvested at that point, and then resuspended into stress culture conditions. For nitrogen starvation and/or high light stress, cells were transferred to nitrate-free mediums and/or new cultures under the continuous illumination at 300 μE/m²s. Therefore, the following combinations of shifts in culture conditions of D. tertiolecta were investigated: high light intensity and nitrate deficiency (HLND), high light intensity and nitrate sufficiency (HLNS), low light intensity and nitrate deficiency (LLND), and low light intensity and nitrate sufficiency (LLNS); the LLNS condition was the same as the initial condition. All treatments were performed in duplicate and the cells were harvested at 0 (control), 12, 36, and 72 h after starting the stress at each phase. When cells were harvested, 400 mL of the cell suspensions were centrifuged at 1500 rpm (UNION 32R PLUS, Hanil Science Industrial. Co., Incheon, Republic of Korea) for 5 min and washed with distilled water (Milli-Q system, Millipore, Bedford, MA, USA). This process was repeated four times. Dry cell weights (DCW) were measured after freeze-drying, and cells were kept in labeled tubes at −80°C for storage.

Lipid Extraction

Glycerolipids and glycerophospholipids, were extracted with a modified Folch method [17]. Three hundred microliters of methanol and 600 µL of chloroform were added to 1 mg of dried cells and vortexed. Then, the mixture was incubated for 1 h at room temperature in a shaker, and then phase separation was induced by adding 250 µL of water. The mixture was centrifuged at 1,000 g for 10 min and the lower phase was collected using a Pasteur pipet. Then the upper phase was extracted again as was done in the above procedure with 400 µL of the solvent mixture, whose composition was equivalent to the assumed composition of the lower phase (chloroform/methanol/water, 86∶14∶1, v/v/v) and then combined with the first lower phase. Five hundred microliters of the combined organic phase aliquot was taken and then dried with nitrogen gas. The dried extracts were stored at −80°C for subsequent analysis.

A method based on Bohoyo-Gil et al [18] and Scaife et al [19] were modified to extract carotenoids from D. tertiolecta. Initially, 10 mg of dried D. tertiolecta sample was extracted with 1 mL of acetone and vortexed for 20 sec. After a 5 min sonication, the tube was centrifuged at 1,000 g for 10 min at 4°C (Model 1730MR, Gyrozen Co., Ltd, Daejeon, Republic of Korea). The extraction was repeated twice until colorless. The supernatant was collected separately from each sample and the extracts were passed through syringe filters (1.7 cm²) with a 0.45-μm pore size polytetrafluoroethylene (PTFE) membrane (Minisart SRP 15, Sartorius, Germany). After filtration, 0.7 mL of each sample solution was transferred into vials and 0.7 mL of hexane with 0.1% BHT and 0.2 mL of water were added and the mixture was vigorously shaken. The 0.5 mL hexane layer (upper layer) was separated, and this extract was dried under a nitrogen gas flow and reconstituted in 0.5 mL of acetonitrile: methanol (70∶30, v/v). All extracts were protected from light and treated using amber glass vials with screw caps to avoid degradation of carotenoids.

Analysis of Glycerolipids and Glycerophospholipids

Lipid analysis was performed using electrospray ionization-tandem mass spectrometry (ESI-MS/MS) on a linear ion-trap mass spectrometer (LTQ-XL, ThermoFisher Scientific, San Jose, CA, USA) equipped with an automated nanoinfusion/nanospray source (Triversa NanoMate System, Advion, Ithaca, NY, USA). The ionization voltage was set to 1.4 kV, gas pressure to 0.4 psi, and the source was controlled by Chipsoft 8.3.1 software (Advion, Ithaca, NY, USA). All lipid samples were dissolved in 500 µL of methanol: chloroform (9∶1, v/v) containing 7.5 mM ammonium acetate, and 5 μL of the reconstituted samples were infused. The infusion of the extracted lipid samples was performed using an Advion ESI chip with 5.5 μm ID emitter nozzles.

Fifteen microliters of each sample were randomly loaded into a 96-well plate (Eppendorf, Germany) to avoid bias in analysis. A standard mixture of glycerophospholipids (PC, PE, PI, and PG at concentrations of 250 µg/mL each) and a quality-control (QC) sample (pooled sample) were loaded at the beginning, middle, and end of the batch. The 96-well plate was sealed with aluminum film (ThermoFisher Scientific, San Jose, CA, USA) using a heat sealer and then placed on the NanoMate cooling plate, which was set to 5°C to prevent evaporation of the solvent. Full-scan spectra were collected at the 400–1200 m/z and the 500–1300 m/z ranges in positive and negative-ion mode, respectively. The capillary temperature was set to 200°C. The tube lens was set to 100 V. The mass spectra of each sample were acquired in profile mode over 2 min. A collision-induced dissociation (CID) was performed with over an isolated width of 3 m/z units, with 35% collision energy. The tandem mass spectrometry (MS/MS) triggering threshold was set to 500, with a default charge state of 1. Dynamic exclusion parameters were a 60 s repeat duration, 60 s exclusion duration, and a 50 exclusion list size.

Analysis of Carotenoids

The ultra-performance liquid chromatography (UPLC) system was an Accela LC (ThermoFisher Scientific, San Jose, CA, USA) equipped with a degasser, Accela 600 pump, and Accela AS autosampler. The column was selected as a Zorbax Eclipse plus C18 column, 2.1 mm×100 mm and 1.8 µm particle size from Agilent Technologies (Böblingen, Germany) [18], and the column oven was set to a temperature of 25°C. The temperature of the auto-sampler tray was set to 4°C. The injection volume was 5 µL for each reconstituted sample and the elution flow rate was set to a constant 200 µL/min. The mobile phases were a 70∶20∶10 mixture of acetonitrile/methanol/MTBE (solvent A) and 0.1% formic acid (solvent B). The gradient program was as follows: 75% A for 4 min, followed by 98% A between 12 and 40 min. The equilibrium time was 5 min after each run, with 75% solvent A. The separation efficiency of UPLC was evaluated with the separation factor (α) and resolution (Rs).

The LC effluent was pumped through a Accela photodiode array (PDA) (ThermoFisher Scientific, San Jose, CA, USA) and LTQ-XL equipped with an atmospheric chemical ionization (APCI) source. It was reported that APCI is a more powerful technique for ionizing the carotenoids than electrospray ionization (ESI) or the atmospheric pressure photoionization (APPI) [20]. The flow rates of the nitrogen sheath gas and auxiliary gas were set to 25 and 5 (arbitrary units), respectively. The capillary temperature was 275°C and the temperature of the APCI vaporizer was set to 350°C. The voltages of the source, capillary, and tube lens were 6 kV, 41 V, and 100 V, respectively, in positive mode. Mass spectrometer was operated in full-scan mode from 500–700 m/z.

Carotenoid Standards

One hundred micrograms per mililiter of standard stock solutions were prepared in hexane containing 0.1% BHT (w/v) in order to increase solution stability [18], [21]. Solutions were stored in amber vials at −80°C. Standard working solutions were prepared from the stock solution by the appropriate dilution in acetonitrile/methanol (70∶30, v/v). Standard mixtures were analyzed on three separate days to assess inter-day variability, which was performed by measuring the retention time (RT) and area ratio of carotenoids on the three different days, with three determinations each day.

Data Acquisition and Statistical Analysis

MS/MS spectra were analyzed manually for the identification of lipid species using an in-house library, which was built using lipid standards and authentic references. The nominal ion mass spectra, which averaged the scans between 0.5–1.0 min, was extracted using Xcalibur software (ThermoFisher Scientific, San Jose, CA, USA). To normalize the spectrum, the average of the sum of intensities from the QC samples was divided by the sum of the intensities of each sample spectra, and then each value (fold) was multiplied by the intensity of each lipid species in that sample.

The normalized intensities of lipid species under stress conditions were compared to that under control conditions (LLNS). Significant differences in intensity levels of lipid species were determined by one-way analysis of variance using PASW Statistics 18 software (IBM, Somers, NY, USA), followed by the Tukey's significant-difference test. The level of statistical significance was set at p<0.05.

Identification and Quantification of Carotenoids

The identification of various carotenoids was accomplished by a comparison of the retention times and the value of [M+H]⁺ with standards and sample peaks. The standard curves were obtained using a UPLC-PDA system (measuring at 460 nm) [18]. An internal standard, β-apo-8′-carotenal, was added to various concentrations of each carotenoids standards for quantification at a fixed concentration (2.5 µg/mL) [22]. Five concentrations, 0.3125, 0.625, 1.25, 2.5, and 5 µg/mL, were prepared for VIO and αCAR, 0.625, 1.25, 2.5, 5, and 10 µg/mL for γCAR and βCAR, 1.25, 2.5, 5, 10, and 20 µg/mL for NEO and ANT, and 3.125, 6.25, 12.5, 25, and 50 µg/mL for LUT. Next, all standard solutions were injected into the UPLC in triplicate. The standard calibration curves were obtained by plotting concentration against area ratio between components and the internal standard using Quanbrowser (Version 2.5.6, Thermo Electron Corporation, Hemel Hempstead, UK). The limit of detection (LOD) and the limit of quantitation (LOQ) were determined at signal-to-noise ratios of 3.3 and 10, respectively, as described in a previous report [23].

Results and Discussion

1. Identification of Glycerolipids and Glycerophospholipids

A representative pooled sample of D. tertiolecta was used for identifying lipids. In the positive mode, 12 of DGTS, 2 of lyso-DGTS, 1 of MGDG, and 6 of DGDG were detected (Fig. 1A, Table 1). DGTS is analyzed in positive ionization mode as [M + H]⁺, while MGDG and DGDG were detected as [M + Na]⁺. The specific diagnostic ions were used to identify each class of lipids. For example, DGTS species were identified by the fragment as an ion at m/z 236, corresponding to its polar head group of [C₁₀H₂₂NO₅] (Fig. S1) [7].

Figure 1. Analyses of glycerolipids and glycerophospholipids extracted from D. tertiolecta by direct infusion into electrospray ionization-tandem mass spectrometry (ESI-MS/MS) in both the (A) positive- and (B) negative-ion modes.

DGTS, diacylglyceryltrimethylhomoserine; MGDG, monogalactosyldiacylglycerol; DGDG, digalactosyldiacylglycerol; SQDG, sulfoquinovosyldiacylglycerol; PG, phosphatidylglycerol; PI, phosphatidylinositol. m/z, mass-to-charge ratio. NL, normalized intensity level.

Table 1. Identification of lipid species from D. tertiolecta by ESI-tandem mass spectrometry equipped with an automated nanoinfusion/nanospray source.

No.	Lipid molecular speciesa (total acyl carbons: total double bonds)	Proposed composition	Ion mode	Ion species	Nominal mass used in lipid profiling	Theoret-ical exact mass (Da)	Element composition
1	DGTS 30∶2	C 16∶0/14∶2	(+)	[M + H]+	680	680.5465	C40H74O7N+
2	DGTS 32∶4	C 16∶0/16∶4	(+)	[M + H]+	705	704.5465	C42H74O7N+
3	DGTS 32∶2	C 16∶0/16∶2	(+)	[M + H]+	709	708.5778	C42H78O7N+
4	DGTS 34∶4	C 18∶4/16∶0	(+)	[M + H]+	733	732.5778	C44H78O7N+
5	DGTS 34∶3	C 18∶3/16∶0	(+)	[M + H]+	735	734.5935	C44H80O7N+
6	DGTS 34∶2	C 18∶2/16∶0	(+)	[M + H]+	737	736.6091	C44H82O7N+
7	DGTS 34∶1	C 18∶1/16∶0	(+)	[M + H]+	739	738.36248	C44H84O7N+
8	DGTS 36∶6	C 18∶3/18∶3	(+)	[M + H]+	757	756.5778	C46H78O7N+
9	DGTS 36∶5	C 18∶2/18∶3	(+)	[M + H]+	759	758.5935	C46H80O7N+
10	DGTS 36∶4	C 18∶2/18∶2	(+)	[M + H]+	761	760.6091	C46H82O7N+
11	DGTS 36∶3	C 18∶1/18∶2	(+)	[M + H]+	762	762.6248	C46H84O7N+
12	DGTS 36∶2	C 18∶1/18∶1, C18∶2/C18∶0	(+)	[M + H]+	765	764.6404	C46H86O7N+
13	LysoDGTS 16∶0	C 16∶0	(+)	[M + H]+	475	474.3795	C26H52O6N+
14	LysoDGTS 18∶3	C18∶3	(+)	[M + H]+	497	496.3638	C28H50O6N+
15	MGDG 34∶7	C 18∶3/16∶4	(+)	[M + Na]+	768	767.4710	C43H68O10Na+
			(−)	[M + OAc]−	804	803.4946	C45H71O12 −
16	MGDG 34∶6	C 18∶3/16∶3	(−)	[M + OAc]−	806	805.5102	C45H73O12 −
17	DGDG 34∶7	C 18∶3/16∶4	(+)	[M + Na]+	930	929.5238	C49H78O15Na+
18	DGDG 34∶6	C 18∶3/16∶3	(+)	[M + Na]+	932	931.5395	C49H80O15Na+
19	DGDG 34∶5	C 18∶3/16∶2	(+)	[M + Na]+	935	933.5551	C49H82O15Na+
20	DGDG 34∶4	C 18∶4/16∶0, C18∶3/16∶1, C18∶2.16∶2, C18∶1/16∶3	(+)	[M + Na]+	936	935.5708	C49H84O15Na+
21	DGDG 34∶3	C 18∶3/16∶0	(+)	[M + Na]+	938	937.5864	C49H86O15Na+
22	DGDG 34∶2	C 18∶2/16∶0	(+)	[M + Na]+	940	939.6021	C49H88O15Na+
23	SQDG 32∶0	C 16∶0/16∶0	(−)	[M – H]−	794	793.5136	C41H77O12 S−
24	SQDG 34∶3	C 18∶3/16∶0	(−)	[M – H]−	816	815.4972	C43H75O12 S−
25	SQDG 34∶2	C 18∶2/16∶0	(−)	[M – H]−	818	817.5136	C43H77O12 S−
26	SQDG 34∶1	C 18∶1/16∶0	(−)	[M – H]−	820	819.5292	C43H79O12 S−
27	PI 34∶1	C 18∶1/16∶0	(−)	[M – H]−	836	835.5337	C43H80O13 P−
28	PG 34∶3	C 18∶3/16∶0	(−)	[M – H]−	744	743.4863	C40H72O10 P−
29	PG 34∶2	C 18∶1/16∶1	(−)	[M – H]−	746	745.5020	C40H74O10 P−

^aDGTS, diacylglyceryltrimethylhomoserine; MGDG, monogalactosyldiacylglycerol; DGDG, digalactosyldiacylglycerol; SQDG, sulfoquinovosyldiacylglycerol; PI, phosphatidylinositol; PG, phosphatidylglycerol.

The fragment at m/z 243, corresponding to the galactosyl head group of [C₉H₁₆O₆+ Na]⁺, was used for the identification of MGDG species (Fig. S2) [7]. Tandem-MS analysis of sodiated DGDG species in the positive-ion mode led to a fragment at m/z 405, corresponding to the digalactosyl head group of [C₁₅H₂₆O₁₁+ Na]⁺ (Fig. S3) [7]. The neutral losses of 162 Da, corresponding to the loss of the hexose moiety [C₆H₁₀O₅] of the head group, occurred in both MGDG and DGDG. In the positive ion mode of the MS/MS spectrum, lyso-glycerolipid or dehydrated lyso-glycerolipid product ions were observed remarkably well, and therefore they were used to identify the fatty acyl groups of each lipid species.

In the negative mode, 2 of MGDG, 4 of SQDG, 1 of PI, and 2 of PG were detected (Fig. 1B, Table 1). MGDG can be detected in the positive mode, but was strongly detected in the negative mode as [M + OAc]⁻ in negative mode. On the other hand, SQDG, PI, and PG were ionized in the negative mode by forming [M − H]⁻.

The major diagnostic ion for MGDG in the negative-ion mode is m/z 253, corresponding to galactosylglycerol of [C₉H₁₆O₈]⁻ (Fig. S4). SQDG species were identified by the fragment ion at m/z 225, corresponding to the sulfoquinovosyl head group of [C₆H₉O₇S]⁻ (Fig. S5) [8]. The PI species resulted in the three common fragment ions at m/z 241, 297, and 315, corresponding to the dehydrated phosphoinositol group of [C₆H₁₀O₈P]⁻, the epoxide anion form of the phosphoinositol head group of [C₉H₁₄O₉P]⁻, and the dehydrated glycerophosphoinositol group of [C₉H₁₆O₁₀P]⁻, respectively. A neutral loss of 180 Da, corresponding to the inositol group of [C₆H₁₂O₆] from the head group, was also observed (Fig. S6) [24], [25]. The neutral loss of 74 Da, corresponding to the loss of a dehydrated glycerol [C₃H₆O₂]⁻, was used for the identification of PG species (Fig. S7) [26].

In the negative mode, since fatty acids were ionized as carboxylate ions, they could easily be identified after fragmentation, although their positions on glycerol backbone (sn-1 and sn-2) or the cis/trans configuration could not be determined by this type of analysis.

2. Changes in Lipid Profiles under Stress Conditions

A principal component analysis (PCA) was conducted on D. tertiolecta samples using normalized intensities of identified lipid species. The PCA score plot showed a clear separation according to stress conditions when using the compounds detected in the positive-ion mode rather than lipid species in the negative-ion mode. In particular, samples under each stress condition were efficiently distinguished from control conditions (LLNS) at the stationary phase (Fig. S8). As a consequence, variation of the lipid composition at the stationary phase was investigated in more detail. The ratio was calculated using the normalized intensities of identified lipids under each stress condition compared to those under control conditions, and the statistical significance was tested (Table 2). The actual values of the normalized intensities of lipid species were described in Table S1.

Table 2. The ratio of intensity of identified lipid species in D. teriolecta under other culture conditions compared to that intensity of them under control condition (low light and nitrate sufficient, LLNS) at the stationary phases.

			Culture conditiona
			LLND			HLNS			HLND
			12h	36h	72h	12h	36h	72h	12h	36h	72h
No.	Compoundsb	Proposed composition
1	DGTS 30∶2	C 16∶0/14∶2	1.7 n.s.	0.9 n.s.	1.4 n.s.	1.6 n.s.	0.8 n.s.	1.1 n.s.	1.5 n.s.	1.0 n.s.	1.8 n.s.
2	DGTS 32∶4	C 16∶0/16∶4	1.9 n.s.	1.1 n.s.	1.2 n.s.	1.1 n.s.	0.8 n.s.	0.8 n.s.	1.6 n.s.	0.6 ∇	0.7 n.s.
3	DGTS 32∶2	C 16∶0/16∶2	1.4 n.s.	1.3 n.s.	1.4 n.s.	1.1 n.s.	1.2 n.s.	1.1 n.s.	1.2 n.s.	1.0 n.s.	1.3 n.s.
4	DGTS 34∶4	C 18∶4/16∶0	1.4 n.s.	0.5 ∇	0.6 n.s.	1.3 n.s.	0.8 ∇	0.8 n.s.	1.7 n.s.	0.3 ∇	0.5 n.s.
5	DGTS 34∶3	C 18∶3/16∶0	2.0 n.s.	1.0 n.s.	1.2 n.s.	1.7 n.s.	1.1 n.s.	1.1 n.s.	1.7 n.s.	0.7 n.s.	1.2 n.s.
6	DGTS 34∶2	C 18∶2/16∶0	2.8 n.s.	2.1 ▴	1.8 n.s.	1.9 n.s.	1.4 n.s.	1.4 n.s.	2.7 n.s.	2.0 ▴	2.7 ▴
7	DGTS 34∶1	C 18∶1/16∶0	2.6 n.s.	2.0 ▴	1.5 n.s.	1.7 n.s.	1.3 n.s.	1.1 n.s.	2.4 n.s.	1.9 ▴	2.1 ▴
8	DGTS 36∶6	C 18∶3/18∶3	1.7 n.s.	0.6 ∇	0.9 n.s.	1.1 n.s.	0.6 ∇	0.7 n.s.	1.7 n.s.	0.3 ∇	0.7 n.s.
9	DGTS 36∶5	C 18∶2/18∶3	2.0 n.s.	0.8 n.s.	1.3 n.s.	1.6 n.s.	0.8 n.s.	0.9 n.s.	2.1 n.s.	0.4 ∇	1.3 n.s.
10	DGTS 36∶4	C 18∶2/18∶2	2.2 n.s.	1.0 n.s.	1.2 n.s.	1.9 n.s.	1.0 n.s.	0.9 n.s.	2.0 n.s.	0.6 ∇	1.5 ▴
11	DGTS 36∶3	C 18∶1/18∶2	1.2 n.s.	0.9 n.s.	1.0 n.s.	0.9 n.s.	0.7 n.s.	0.6 n.s.	1.0 n.s.	0.5 ∇	0.9 n.s.
12	DGTS 36∶2	C 18∶1/18∶1, C18∶2/C18∶0	0.9 n.s.	1.0 n.s.	1.1 n.s.	0.9 n.s.	0.9 n.s.	0.8 n.s.	0.8 n.s.	0.8 n.s.	1.0 n.s.
13	LysoDGTS 16∶0	C 16∶0	1.1 n.s.	1.1 n.s.	0.9 n.s.	1.2 n.s.	1.3 n.s.	1.0 n.s.	0.8 n.s.	0.9 n.s.	0.9 n.s.
14	LysoDGTS 18∶3	C 18∶3	1.1 n.s.	0.8 n.s.	0.6 n.s.	1.2 n.s.	1.0 n.s.	0.7 n.s.	1.0 n.s.	0.7 n.s.	0.5 ∇
15	MGDG 34∶7	C 18∶3/16∶4	0.4 n.s	1.3 n.s.	0.8 n.s.	0.4 n.s.	1.0 n.s.	0.5 n.s.	0.5 n.s.	1.0 n.s.	0.6 n.s.
			0.8 n.s.	1.0 n.s.	1.0 n.s.	1.0 n.s.	0.6 n.s.	0.9 n.s.	0.8 n.s.	0.8 n.s.	1.0 n.s.
16	MGDG 34∶6	C 18∶3/16∶3	0.7 n.s.	1.0 n.s.	1.1 n.s.	1.1 n.s.	0.9 n.s.	1.1 n.s.	1.0 n.s.	1.0 n.s.	1.3 n.s.
17	DGDG 34∶7	C 18∶3/16∶4	0.8 n.s.	1.7 n.s.	1.7 ▴	0.9 n.s.	1.5 n.s.	1.3 n.s.	0.8 n.s.	1.9 n.s.	1.6 ▴
18	DGDG 34∶6	C 18∶3/16∶3	0.7 n.s.	1.7 n.s.	1.7 ▴	0.8 n.s.	1.5 n.s.	1.4 ▴	0.7 n.s.	2.1 n.s.	1.9 ▴
19	DGDG 34∶5	C 18∶3/16∶2	0.7 n.s.	2.2 n.s.	1.8 ▴	0.8 n.s.	1.5 n.s.	1.4 ▴	0.9 n.s.	3.0 ▴	2.5 ▴
20	DGDG 34∶4	C 18∶4/16∶0, C18∶3/16∶1, C18∶2.16∶2, C18∶1/16∶3	0.7 n.s.	2.0 n.s.	1.7 ▴	0.7 n.s.	1.4 n.s.	1.3 ▴	0.9 n.s.	2.7 ▴	2.1 ▴
21	DGDG 34∶3	C 18∶3/16∶0	0.7 n.s.	1.1 n.s.	1.0 n.s.	0.6 n.s.	0.8 n.s.	0.8 ∇	0.7 n.s.	1.1 n.s.	0.9 n.s.
22	DGDG 34∶2	C 18∶2/16∶0	0.7 n.s.	0.8 n.s.	0.7 n.s.	0.6 n.s.	0.6 ∇	0.7 n.s.	0.7 n.s.	0.6 ∇	0.5 n.s.
23	SQDG 32∶0	C 16∶0/16∶0	0.9 n.s.	0.9 n.s.	0.9 n.s.	1.5 n.s.	1.1 n.s.	1.4 n.s.	1.2 n.s.	1.3 ▴	1.6 n.s.
24	SQDG 34∶3	C 18∶3/16∶0	1.0 n.s.	0.8 n.s.	0.7 n.s.	1.4 n.s.	0.9 n.s.	1.0 n.s.	0.9 n.s.	0.7 n.s.	0.7 n.s.
25	SQDG 34∶2	C 18∶2/16∶0	0.9 n.s.	0.8 n.s.	0.9 n.s.	1.6 n.s.	1.1 n.s.	1.1 n.s.	1.3 n.s.	1.0 n.s.	1.0 n.s.
26	SQDG 34∶1	C 18∶1/16∶0	0.9 n.s.	1.1 n.s.	1.0 n.s.	1.8 n.s.	1.6 n.s.	1.5 ▴	1.4 n.s.	1.6 n.s.	1.3 n.s.
27	PI 34∶1	C 18∶1/16∶0	0.6 n.s.	1.3 n.s.	1.1 n.s.	1.2 n.s.	1.2 n.s.	1.3 ▴	1.1 n.s.	1.6 n.s.	1.6 ▴
28	PG 34∶3	C 18∶3/16∶0	0.7 n.s.	0.6 n.s.	0.6 n.s.	1.2 n.s.	0.6 n.s.	0.6 n.s.	0.6 n.s.	0.5 n.s.	0.6 n.s.
29	PG 34∶2	C 18∶1/16∶1	0.8 n.s.	0.9 n.s.	0.9 n.s.	1.4 n.s.	0.9 n.s.	1.0 n.s.	0.8 n.s.	0.9 n.s.	1.0 n.s.

^aLLND, low light intensity and nitrate deficiency; HLNS, high light intensity and nitrate sufficiency; HLND, high light intensity and nitrate deficiency.

^bDGTS, diacylglyceryltrimethylhomoserine; MGDG, monogalactosyldiacylglycerol; DGDG, digalactosyldiacylglycerol; SQDG, sulfoquinovosyldiacylglycerol; PI, phosphatidylinositol; PG, phosphatidylglycerol.

▴, significantly increased level compared with control (p<0.05); ∇, significantly decreased level compared with control (p<0.05); n.s., no significant difference.

A nitrate deficiency had a greater impact on DGTS species than high light intensity (Table 2). When both stress factors were imposed on D. tertiolecta, DGTS species exhibited the largest changes. Nitrogen starvation brought about a strong increase in DGTS species containing monounsaturated fatty acid (MUFA) moieties, such as C18∶1, and one of the PUFAs, C18∶2, while DGTS species exhibited significant decreases in polyunsaturated fatty acid (PUFA) moieties, such as C18∶3 and C18∶4. D. tertiolecta cultures grown under high light attained changes in DGTS species containing C18∶3 and C18∶4 only.

D. tertiolecta grown under HLND showed a significant decrease in DGTS species consisting of C18 fatty acyl groups. The levels of DGTS species, which have the common C16∶0 fatty acid, showed variations depending on fatty acid moieties in other position. DGTS, whose fatty acyl group has four double bonds, such as C16∶4 and C18∶4, were reduced, while DGTS species, whose fatty acyl group includes C18∶1 and C18∶2, resulted in an increase in the intensity. The most intense variations in DGTS species was observed at 36 hours after the culture underwent stress. In addition, lyso-DGTS 16∶0 and lyso-DGTS 18∶3 were detected from D. tertiolecta samples. This may explain how they were the most abundant lyso form of species from among the detected DGTS.

The physiological role of DGTS has not yet been unraveled. According to Sato, it was assumed that DGTS would play a similar role within the cell to that of PC in higher plants because of the similarity in their chemical and physical characteristics, and the fact that the algae, which contains DGTS, rarely stores PC simultaneously [27]. PC acts as an acyl donor to the synthesis of triacylglycerol (TAG), catalyzed by phospholipid: diacylglycerol acyltransferase (PDAT). There are several reports indicating that many microalgae tend to accumulate TAG under nitrate deplete condition [28], [29]. DGTS has a role as a substrate for the desaturation of C18∶1 to 18∶2 and as a donor of primarily C18∶2 to other membrane lipids, such as PE [30]. It could then be hypothesized that the high level of DGTS with C18∶1 and C18∶2 is responsible for the enhancement of the biosynthesis of TAG. Moreover, as shown in Table 2, theses DGTS species commonly have C16∶0, fatty acids relating to energy storage, and concurrently is a predominant fatty acyl chain in TAG. Meanwhile, He et al mentioned that DGTS has been hypothesized to transfer fatty acids from the cytoplasm to chloroplasts [8]. Therefore, a significant reduction in DGTS species with PUFA could be related to an increment in the desaturation of glycerolipids, such as DGDG, which are present in chloroplasts. However, the hypotheses drawn above require verification through subsequent experimental testing.

The changes of DGDG species were observed under both nitrogen starvation and high light intensity. The significant increase of DGDG species containing unsaturated C16 fatty acid was found under these two stress conditions. In high light conditions, the DGDG species, which have a saturated fatty acid, C16∶0, showed a significant decline. When D. tertiolecta cultures were under nitrate deficiency and intense light, the high values of ratio were shown after 36 h in DGDG 34∶4 and DGDG 34∶5.

A neutral lipid, DGDG, facilitates the assembly of photosynthetic complexes and aids in the oligomerization of protein subunits. Consequently, it occupies functionally and structurally crucial sites of both the photosystem I and II (PSI and PSII) reaction centers [31], [32]. Hölzl and Dörmann reported that the head group of galactolipids interacts with photosynthetic protein complex [31]. Recently, the contribution of the fatty acyl chain composition to photosynthesis has been studied. Montero et al hypothesized that high light leads to an improvement in the photosynthetic activity, and it requires a higher membrane fluidity so that there could be an increase in glycerolipid species with desaturated fatty acyl chains [33]. This report would be in accordance with above result regarding the enhancement in DGDG species with unsaturated C16 fatty acyl chain under high light condition (Table 2).

Meanwhile, nitrate deficiency did not lead to any significant changes in SQDG species, while cultivation under a high light intensity resulted in an increase of SQDG (18∶1/16∶0) at 72 h. SQDG (16∶0/16∶0) showed a significant increment at 72 h in D. tertiolecta cultivated under nitrate deficiency and high light intensity. SQDG has an important role in maintaining function and structure of PSII complex, especially when the PSII is present under stress conditions such as high temperature [34]. The major fatty acyl chains of SQDG are known to include C16∶0 and C18∶1 in green algae [35] so that it might be related to the characteristic changes observed in SQDG with C16∶0 and/or C18∶1 under high light stress in our results.

PI 34∶1 was found to be increased at 72 h under HLNS and HLND conditions. PI was present at a low abundance in plants, and is related to cell signaling rather than acting as a structural component of the membrane. In particular, there are several reports that various kinds of phosphoinositides, which are generated from PI, were produced rapidly in response to osmotic, drought, and salt stresses [36]. However, to our knowledge, the alteration of PI by high light stress has not been reported yet.

MGDG and PG species did not exhibit variations under stress conditions, despite previous findings indicating that they have been found to play an essential role in photosynthesis [33].

3. LC-MS Analysis of Carotenoids

Figure 2 shows the LC-MS chromatogram of carotenoids extracted from D. tertiolecta. Seven carotenoids were separated simultaneously within 40 min. Table 3 shows the assignment data for carotenoids in D. tertiolecta. All of the carotenoids ionized by APCI showed the protonated molecular ion [M + H]⁺: 601.4 for neoxanthin and violaxanthin, 585.4 for anthraxanthin, 569.4 for lutein, and 537.4 for γ-, α-, and β-carotene. The UPLC system coupled with the PDA allowed us to distinguish carotenoids species according to chromatographic retention time, although there were some carotenoids that produced an identical m/z of [M + H]⁺. The separation factors (α) and resolutions (Rs) of carotenoids in the algae for all peaks were higher than 1. Furthermore, reproducibility was good for these carotenoids as presented in the analytical results, with an relative standard deviation (R.S.D.) <0.91% for retention times and an R.S.D. <7.63% for integrated areas.

Figure 2. Analyses of (A) carotenoid standard mixture and (B) carotenoids extracted from D. tertiolecta by ultra-performance liquid chromatography (UPLC) equipped with a photodiode array (PDA) in positive-ion mode.

NEO, neoxanthin; VIO, violaxanthin; ANT, anthraxanthin; ZEA, zeaxanthin; LUT, lutein; βCRYP, β-cryptoxanthin; LYC, lycopene; NEU, neurosporene; γCAR, γ-carotene; αCAR, α-carotene; βCAR, β-carotene; IS*, internal standard (β-apo-8′-carotenal). mAU, mili-absorbance unit.

Table 3. List of identified carotenoids in D. tertiolecta.

Peak no.	Compound a	Formula	m/z [M + H]+	RT (min) b	R.S.D. RT (%)	R.S.D. area ratio (%)	α c	Rs d
1	NEO	C40H56O4	601.4	8.06	0.91	2.00	–	–
2	VIO	C40H56O4	601.4	9.43	0.58	0.99	1.20 (1, 2)e	3.75 (1, 2)
3	ANT	C40H56O3	585.4	11.51	0.65	1.36	1.25 (2, 3)	5.94 (2, 3)
4	LUT	C40H56O2	569.4	13.28	0.61	1.91	1.17 (3, 4)	4.72 (3, 4)
5	IS*			16.82	–	–	1.29 (4, 5)	10.41 (4, 5)
6	γCAR	C40H56	537.4	30.43	0.51	7.63	1.87 (5, 6)	18.77 (5, 6)
7	αCAR	C40H56	537.4	35.43	0.50	3.72	1.17 (6, 7)	5.10 (6, 7)
8	βCAR	C40H56	537.4	36.75	0.84	1.61	1.04 (7, 8)	1.61 (7, 8)

The mass-to-charge ratio, retention time (RT), reproducibility (R.S.D. RT and R.S.D. area ratio), separation factor (α), and resolution (Rs) were described.

^aNEO, neoxanthin; VIO, violaxanthin; ANT, anthraxanthin; LUT, lutein; γCAR, γ-carotene; αCAR, α-carotene; βCAR, β-carotene; IS*, internal standard.

^bData were obtained from inter-day variability testing performed by measuring the RT and area ratio of carotenoids on the three different days, with three determinations each day, for total of nine determinations.

^cSeparation factor (α)  =  (RT_n+1-RT₀)/(RT_n-RT₀), where RT_n is the retention time of a compound, and RT₀ is the retention time of an unretained peak.

^dRs  =  2 (RT_n+1-RT_n)/(W_n+W_n+1), where W_n is the band width of a compound at the baseline.

^eValues in parentheses represent two neighboring peaks.

To quantify carotenoids in D. tertiolecta, calibration curves, the related LOD, and LOQ were obtained (Table S2). Linearity was obtained using five calibrators and three replicates. The LOD and LOQ were measured at the peak-area ratio between the analytes and internal standards against the analyte concentrations. The correlation coefficients of each calibration curve ranged from 0.9959 to 0.9999.

4. Changes in Carotenoid Profiles under Stress Conditions

The total carotenoid content (mg/g DCW) and productivity (mg/L) are illustrated in Figure 3. The results indicated that the higher level of total carotenoid content was obtained when the stress was triggered at the stationary phase (Fig. 3A). The maximum level of total carotenoid content was showed at LLNS (control) at the stationary phase. It appeared that carotenoid accumulation under control conditions increased over time, but the carotenoid content didn't show certain tendencies under other stress conditions. The greatest enhancement of the total carotenoid content was observed in D. tertiolecta under LLNS followed by HLNS, LLND, and HLND at the stationary phase at 72 h (ST-72 h).

Figure 3. Effect of high light intensity and nitrate deficiency on the (A) total carotenoid content (mg/g dry cell weight) and (B) productivity (mg/L) of D. tertiolecta under shifted cultivation conditions at the exponential (EX) and stationary (ST) phases.

Vertical bars represent the standard deviations (n = 4). Different alphabet in the same time point represent significant differences (p<0.05) within samples under various culture conditions. Sharing the same alphabet indicates no significant differences among levels in carotenoid content or productivity. LLNS, low light intensity and nitrate sufficiency; LLND, low light intensity and nitrate deficiency; HLNS, high light intensity and nitrate sufficiency; HLND, high light intensity and nitrate deficiency.

The carotenoid content of seven carotenoid species (neoxanthin, violaxanthin, anthraxanthin, lutein, and γ-, α-, and β-carotene) was analyzed and described in Table 4. The result of the changes in the carotenoid content over time and under culture conditions was similar to the total carotenoid variation, with the exception of γ-carotene. The level of γ-carotene content was increased under nitrate deficient conditions. However, the particular physiological functions of γ-carotene are not yet well understood.

Table 4. Time-course quantification of carotenoids^a in D. tertiolecta under the various culture conditions^b shifted at the exponential (EX) and stationary (ST) phases.

			0h				12h				36h				72h
			LLNS	LLND	HLNS	HLND	LLNS	LLND	HLNS	HLND	LLNS	LLND	HLNS	HLND	LLNS	LLND	HLNS	HLND
NEO	EX	Content (mg/g)	1.76±0.01a	1.58±0.00b	1.73±0.01a	1.71±0.03a	1.96±0.03a	1.69±0.22ab	1.38±0.19b	0.94±0.08c	2.64±0.16a	1.10±0.00bc	1.31±0.13b	0.96±0.02c	4.09±0.06a	0.98±0.12b	1.25±0.21b	0.68±0.10c
		Productivity (mg/L)	0.45±0.00a	0.50±0.00b	0.46±0.00a	0.55±0.01c	0.52±0.04	0.55±0.07	0.59±0.13	0.44±0.05	0.95±0.04a	0.38±0.00b	1.33±0.22c	0.55±0.01b	3.36±0.02a	0.74±0.06 b	2.66±0.44c	0.61±0.07 b
	ST	Content (mg/g)	2.84±0.30a	3.65±0.43b	4.32±0.07bc	3.41±0.41ab	3.38±0.15ab	3.44±0.14a	3.14±0.10b	2.22±0.09c	4.28±0.21a	3.09±0.03b	2.75±0.28b	2.14±0.11c	7.50±0.71a	3.24±0.12c	4.25±0.59b	1.85±0.04d
		Productivity (mg/L)	2.29±0.08a	2.75±0.38b	3.19±0.06c	2.55±0.19ab	2.75±0.13	2.92±0.20	3.07±0.42	2.62±0.18	3.86±0.16a	3.28±0.04ab	3.85±0.68a	2.77±0.13b	5.44±0.84a	3.30±0.08b	6.74±0.59 c	2.54±0.16 b
VIO	EX	Content (mg/g)	0.35±0.00a	0.35±0.00a	0.39±0.01b	0.32±0.00c	0.29±0.02a	0.20±0.01b	0.38±0.01c	0.19±0.02b	0.35±0.04a	0.21±0.00b	0.35±0.02a	0.38±0.02a	0.56±0.05a	0.33±0.03b	0.49±0.02a	0.32±0.05b
		Productivity (mg/L)	0.09±0.00a	0.11±0.00b	0.10±0.00c	0.10±0.0 c	0.08±0.01a	0.07±0.00a	0.16±0.02b	0.09±0.01a	0.12±0.01a	0.07±0.00b	0.35±0.01c	0.22±0.01d	0.46±0.04 a	0.25±0.01 b	1.04±0.03 c	0.29±0.04b
	ST	Content (mg/g)	0.61±0.05a	0.52±0.01a	0.73±0.02b	0.50±0.08a	0.72±0.02a	0.66±0.02a	0.67±0.01a	0.47±0.05b	0.55±0.07	0.57±0.01	0.62±0.07	0.61±0.04	0.92±0.10a	0.54±0.07b	0.62±0.09b	0.51±0.06b
		Productivity (mg/L)	0.49±0.01ab	0.39±0.01a	0.54±0.01b	0.38±0.10a	0.59±0.01	0.56±0.03	0.66±0.10	0.55±0.04	0.49±0.05a	0.61±0.01ab	0.86±0.17c	0.79±0.06bc	0.67±0.11a	0.55±0.06a	0.99±0.04b	0.71±0.11a
ANT	EX	Content (mg/g)	0.38±0.01a	0.35±0.00b	0.35±0.00b	0.34±0.00b	0.47±0.02a	0.36±0.01b	0.48±0.03a	0.37±0.01b	0.49±0.03a	0.33±0.00b	0.43±0.03a	0.52±0.04a	0.59±0.02a	0.32±0.00c	0.37±0.04bc	0.38±0.02b
		Productivity (mg/L)	0.10±0.00a	0.11±0.00b	0.09±0.00a	0.11±0.00b	0.12±0.01a	0.12±0.00a	0.21±0.03b	0.17±0.01b	0.18±0.01a	0.11±0.00b	0.44±0.01c	0.30±0.02d	0.49±0.01a	0.24±0.01b	0.79±0.10c	0.34±0.01b
	ST	Content (mg/g)	0.68±0.07a	0.67±0.03a	0.81±0.01b	0.63±0.04a	0.69±0.01a	0.66±0.01a	0.69±0.03a	0.63±0.01b	0.67±0.02a	0.67±0.01a	0.66±0.07a	0.78±0.04b	1.09±0.07a	0.79±0.06c	0.81±0.06bc	0.80±0.04c
		Productivity (mg/L)	0.55±0.01ab	0.51±0.01ab	0.60±0.01a	0.48±0.08b	0.56±0.01a	0.56±0.02a	0.68±0.08b	0.74±0.01b	0.60±0.00a	0.71±0.01a	0.93±0.17b	1.01±0.05b	0.79±0.10a	0.80±0.05a	1.29±0.14b	1.11±0.11b
LUT	EX	Content (mg/g)	8.35±0.02a	7.47±0.04b	7.64±0.00c	7.30±0.04d	8.49±0.09a	7.54±0.05b	7.88±0.62ab	5.36±0.33c	10.10±0.28a	5.36±0.03b	7.05±0.13c	6.63±0.30b	14.95±0.13a	5.41±0.35b	6.27±0.13c	5.07±0.65b
		Productivity (mg/L)	2.13±0.01a	2.37±0.01b	2.03±0.00c	2.36±0.01b	2.24±0.14a	2.47±0.03a	3.37±0.56b	2.53±0.24a	3.62±0.03a	1.83±0.01b	7.09±0.61c	3.80±0.17a	12.29±0.21a	4.10±0.10b	13.36±0.23c	4.59±0.40b
	ST	Content (mg/g)	14.17±1.89ab	15.14±0.33ac	16.49±0.09c	12.73±0.15b	13.32±0.65a	13.22±0.53a	12.88±0.30a	8.92±0.28b	13.00±0.15a	12.76±0.45a	13.03±0.64a	11.52±0.52b	22.71±1.16a	13.26±0.36b	16.33±1.21c	10.91±0.10d
		Productivity (mg/L)	11.39±0.70a	11.40±0.04a	12.19±0.15a	9.56±0.94b	10.85±0.50ab	11.22±0.74ab	12.60±1.67a	10.51±0.64b	11.72±0.16a	13.53±0.66ab	18.16±2.26c	14.90±0.65b	16.43±1.80a	13.48±0.18a	26.03±3.05b	15.00±0.75a
γCAR	EX	Content (mg/g)	0.20±0.03	0.20±0.00	0.20±0.00	0.19±0.00	0.07±0.00a	0.23±0.02b	0.14±0.01c	0.17±0.01d	0.07±0.01a	0.36±0.04b	0.22±0.00c	0.30±0.07bc	0.59±0.07a	0.77±0.14b	0.47±0.03a	0.48±0.04a
		Productivity (mg/L)	0.05±0.01	0.06±0.00	0.05±0.00	0.06±0.00	0.02±0.00a	0.08±±0.01b	0.06±0.00c	0.08±0.01b	0.02±0.00a	0.12±0.01b	0.22±0.02c	0.17±±0.04bc	0.49±0.06ab	0.58±0.08a	1.00±0.06c	0.43±0.03b
	ST	Content (mg/g)	1.01±0.04a	1.24±0.15b	1.08±0.03a	0.94±±0.02a	1.03±0.05a	1.04±0.06a	0.73±0.03b	0.60±0.03c	1.26±±0.03a	1.27±0.08a	0.47±0.07b	1.16±0.02a	1.17±0.07a	1.47±0.05b	0.81±±0.21c	1.45±0.05b
		Productivity (mg/L)	0.81±0.03ab	0.93±0.09a	0.80±0.03ab	0.71±0.09b	0.84±0.04ab	0.88±0.07a	0.72±0.10b	0.70±0.06b	1.13±0.01a	1.35±0.10b	0.66±0.15c	1.50±0.03b	0.85±0.09 a	1.50±0.07bc	1.34±0.55ab	1.99±0.16c
αCAR	EX	Content (mg/g)	0.12±0.00	0.11±0.00	0.10±0.00	0.08±0.03	0.09±0.02a	0.07±0.01a	0.04±0.00b	0.03±±0.00b	0.10±0.00a	0.05±0.00b	0.06±0.01b	0.06±0.00b	0.15±0.00a	0.05±0.01b	0.05±0.00b	0.07±0.01c
		Productivity (mg/L)	0.03±0.00	0.04±0.00	0.03±0.00	0.02±0.01	0.02±0.01a	0.02±0.00ab	0.02±0.00ab	0.02±0.00b	0.03±±0.00a	0.02±0.00b	0.06±v0.01c	0.04±0.00a	0.12±0.00a	0.04±0.00b	0.10±0.00c	0.07±0.00d
	ST	Content (mg/g)	0.09±0.02a	0.12±0.01b	0.11±0.01ab	0.09±0.00a	0.10±0.01a	0.09±0.00b	0.06±0.00c	0.04±0.00d	0.23±0.08a	0.08±0.00b	0.09±0.00b	0.07±0.00b	0.26±0.02a	0.09±0.00b	0.10±0.00bc	0.08±0.00c
		Productivity (mg/L)	0.07±0.01ab	0.09±0.00c	0.08±0.00ac	0.07±0.00b	0.08±0.01a	0.08±0.00a	0.06±0.01b	0.04±0.00c	0.21±0.07a	0.09±0.00b	0.13±0.01b	0.09±0.00 b	0.19±0.03 a	0.09±0.00b	0.17±0.03a	0.11±0.00b
βCAR	EX	Content (mg/g)	0.79±0.01a	0.79±0.00a	0.63±0.00b	0.73±0.00c	0.64±0.16ab	0.77±0.04a	0.51±0.06b	0.50±0.03b	0.87±0.03a	0.65±0.00b	0.85±0.03a	0.74±0.05b	1.92±0.26a	0.80±0.08b	1.13±0.03c	0.80±0.09b
		Productivity (mg/L)	0.20±0.00a	0.25±0.00b	0.17±0.00c	0.24±0.00d	0.17±0.05a	0.25±0.01ab	0.22±0.04ab	0.24±0.02b	0.31±0.00ab	0.22±0.00 a	0.86±0.09 c	0.42±0.03 b	1.58±0.23 a	0.61±0.04b	2.40±0.07c	0.72±0.05b
	ST	Content (mg/g)	2.06±0.22a	2.38±0.13b	2.40±0.08b	1.98±0.01a	2.15±0.02a	1.95±0.03b	1.78±0.03c	1.27±0.08d	2.76±0.23a	1.94±0.06b	2.06±0.14b	2.04±0.22b	3.27±0.11a	2.01±0.04b	2.87±0.24c	2.20±0.04d
		Productivity (mg/L)	1.66±0.06ab	1.79±0.07a	1.77±0.07a	1.49±0.17b	1.75±0.02	1.65±0.07	1.75±0.24	1.50±0.14	2.49±0.26ab	2.05±0.09a	2.88±0.42b	2.64±0.29ab	2.37±0.21 a	2.05±0.07 a	4.66±1.18b	3.02±0.10a
Total	EX	Content (mg/g)	11.95±0.02a	10.85±0.04b	11.03±0.01c	10.68±0.01d	12.00±0.11a	10.87±0.17b	10.81±0.90b	7.56±0.46c	14.61±0.54a	8.06±0.07b	10.27±0.26c	9.59±0.26b	22.86±0.27a	8.66±0.71b	10.02±0.28c	7.79±0.96b
		Productivity (mg/L)	3.05±0.00a	3.44±0.01b	2.93±0.00c	3.45±0.00b	3.17±0.26a	3.56±0.04a	4.62±0.79b	3.56±0.34 a	5.24±0.08a	2.76±0.02b	10.34±0.96c	5.50±0.15 a	18.79±0.36 a	6.56±0.28 b	21.35±0.51c	7.06±0.58b
	ST	Content (mg/g)	21.46±2.58ab	23.72±0.21ac	25.95±0.23c	20.28±0.42b	21.38±0.56a	21.07±0.78ab	19.96±0.44b	14.14±0.43c	22.74±0.27a	20.38±0.57b	19.70±1.27bc	18.32±0.64c	36.92±2.18a	21.40±0.53b	25.80±1.67c	17.80±0.23d
		Productivity (mg/L)	17.26±0.83a	17.86±0.17a	19.18±0.29a	15.22±1.44b	17.43±0.41	17.87±1.13	19.54±2.61	16.66±0.99	20.51±0.48a	21.61±0.88a	27.48±3.85b	23.70±0.80ab	26.73±3.15a	21.76±0.24a	41.22±5.47b	24.48±1.40a

^aNEO, neoxanthin; VIO, violaxanthin; ANT, anthraxanthin; LUT, lutein; γCAR, γ-carotene; αCAR, α-carotene; βCAR, β-carotene; Total, total carotenoids.

^bLLNS, low light intensity and nitrate sufficiency; LLND, low light intensity and nitrate deficiency; HLNS, high light intensity and nitrate sufficiency; HLND, high light intensity and nitrate deficiency.

Each value represents the mean ± standard deviation (SD) (n = 4)

Different letters in the same time point represent significant differences (p<0.05) within samples under various culture conditions.

Among all detected carotenoids in D. tertiolecta cells, lutein showed the highest level of content. Although D. salina showed a differential response to stresses, such as different salinity levels, compared to D. tertiolecta [37], this result is in agreement with a previous report, in which one of the major carotenoids in D. salina was lutein [21]. In addition, Lamers et al reported that β-carotene accumulation in D. salina was induced upon the shift to an increased light intensity, from 200 to 1,400 μE/m²s whereas lutein concentrations decreased sharply upon light stress [38]. According to this result, it assumed that the degree of high light intensity (300 μE/m²s) in our study might not be enough to increase the accumulation of β-carotene. Geider et al reported that photosynthetic carotenoids (lutein, neoxanthin, violaxanthin, and antheraxanthin) were reduced by nitrogen limitation in D. tertiolecta [39], which coincides with our results.

The total carotenoid productivity of D. tertiolecta at the stationary phase was higher than in the exponential phase, and it increased gradually over time (Fig. 3B). The highest level of total carotenoid productivity was obtained in D. tertiolecta culture under HLNS. This result came as a result of the fact that the highest dried cell weight of D. tertiolecta cell was acquired under this condition (data is not shown). As shown in Table 4, the highest levels of productivity of neoxanthin, violaxanhin, anthraxanthin, lutein, and β-carotene were recorded in HLNS at ST-72 h. However, α-carotene and γ-carotene were shown to have the highest productivity in LLNS at stationary phase at 36 h (ST-36 h) and HLND at ST-72 h, respectively. With the exception of violaxanthin, the other six carotenoids showed about a two-fold increase in the productivity levels at ST-72 h than during the exponential phase at 72 h (EX-72 h) in HLNS condition. Therefore, from the industrial point of view, HLNS conditions would yield the maximum productivity of carotenoids. For the enhancement of productivity in most of the carotenoids, nitrate limitation was unnecessary, whereas γ-carotene showed the highest level of productivity in D. tertiolecta cell culture under high-light and nitrate-deficient conditions.

Conclusion

NanoESI chip based direct infusion and UPLC-PDA-MS were successfully applied to elucidate changes in the lipid and carotenoid composition in D. tertiolecta under different nutrient-concentration and light-intensity conditions. A total of 29 lipids, including various species such as DGTS, MGDG, DGDG, SQDG, PG, and PI, were identified. Moreover, seven species of carotenoids, including neoxanthin, violaxanthin, anthraxanthin, lutein, and γ-, α-, and β-carotene, were qualified and quantified. Each lipid species showed a different variation in the fatty acid saturation, which might be related to photosynthesis in microalgae. A distinct discrimination of the lipid profiling among the stress conditions was shown when stress was induced at the stationary phase (ST) rather than during the exponential phase (EX). Also, the highest total carotenoid content and productivity were attained by inducing at the ST.

This is the first report of glycerolipid, glycerophospholipid, and carotenoid profiling in D. tertiolecta, and could provide new insights into the biological functions of their lipid species in response to nutrient and light stress. Moreover, this study suggested a methodology for identifying individual lipid species by using lipidomics techniques, which might contribute to providing more advanced knowledge for the enhancement of biofuel or biopharmaceutical materials production in microalgae systems.

Supporting Information

Figure S1

Positive-ion ESI tandem mass spectrum of [M + H]⁺ (at m/z 734) for major diacylglyceryltrimethylhomoserine (DGTS) species (C18∶3/C16∶0-DGTS).

(TIF)

Figure S2

Positive-ion ESI tandem mass spectrum of [M + Na]⁺ (at m/z 767) for major monogalactosyldiacylglycerol (MGDG) species (C18∶3/C16∶4-MGDG).

(TIF)

Figure S3

Positive-ion ESI tandem mass spectrum of [M + Na]⁺ (at m/z 931) for major digalactosyldiacylglycerol (DGDG) species (C18∶3/C16∶3-DGDG).

(TIF)

Figure S4

Negative-ion ESI tandem mass spectrum of [M + OAc]⁻ (at m/z 803) for major monogalactosyldiacylglycerol (MGDG) species (C18∶3/C16∶4-MGDG).

(TIF)

Figure S5

Negative-ion ESI tandem mass spectrum of [M – H]⁻ (at m/z 815) for major sulfoquinovosyl-diacylglycerol (SQDG) species (C18∶3/C16∶0-SQDG).

(TIF)

Figure S6

Negative-ion ESI tandem mass spectrum of [M – H]⁻ (at m/z 835) for major phosphatidylinositol (PI) species (C18∶1/C16∶0-PI).

(TIF)

Figure S7

Negative-ion ESI tandem mass spectrum of [M – H]⁻ (at m/z 745) for major phosphatidylglycerol (PG) species (C18∶2/C16∶0-PG).

(TIF)

Figure S8

PCA score plot derived from ESI-MS/MS of D. tertiolecta samples in (A) positive- and (b) negative-ion modes. EX, exponential phase; ST, stationary phase. •, LLNS; ▪, LLND; ▴, HLNS; ▾, HLND. The samples of each group are biological replicates. The time located at the bottom indicates the hours after triggering the stress condition.

(TIF)

Table S1

The normalized ion intensities of the identified lipids over the culture conditions and time course in D. tertiolecta.

(DOCX)

Table S2

Regression equations, correlation coefficients (r² values), LOD, and LOQ of carotenoids.

(DOCX)

Funding Statement

This work was supported by a grant from the National Research Foundation of Korea funded by the Korean government (MEST) (no. NRF-2011–0021055). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.