Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1971 Nov;22(5):760–762. doi: 10.1128/am.22.5.760-762.1971

Improved Method for Virus Structural Polypeptide Analysis on Dissociating Acylamide Gel

P A Boulanger 1,2, R Warocquier 1,2
PMCID: PMC376413  PMID: 5132092

Abstract

A method is described in which polypeptides can be separated, with a high band resolution, by electrophoresis through “pore gradient” acrylamide gel (15 cm in length) in a sodium dodecyl sulfate-urea dissociating system. The applicability of this technique to the analysis of virus structural components was examined with the adenovirus type 2 model system.

Full text

PDF
760

Images in this article

761Image
on p.761

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acheson N. H., Tamm I. Structural proteins of Semliki Forest virus and its nucleocapsid. Virology. 1970 Jun;41(2):321–329. doi: 10.1016/0042-6822(70)90084-x. [DOI] [PubMed] [Google Scholar]
  2. Anker H. S. A solubilizable acrylamide gel for electrophoresis. FEBS Lett. 1970 Apr 16;7(3):293–293. doi: 10.1016/0014-5793(70)80185-5. [DOI] [PubMed] [Google Scholar]
  3. GREEN M., PINA M. BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION, VI. PROPERTIES OF HIGHLY PURIFIED TUMORIGENIC HUMAN ADENOVIRUSES AND THEIR DNA. Proc Natl Acad Sci U S A. 1964 Jun;51:1251–1259. doi: 10.1073/pnas.51.6.1251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Girard M., Marty L., Suarez F. Capsid proteins of Simian virus 40. Biochem Biophys Res Commun. 1970 Jul 13;40(1):97–102. doi: 10.1016/0006-291x(70)91051-x. [DOI] [PubMed] [Google Scholar]
  5. Laver W. G. Isolation of an arginine-rich protein from particles of adenovirus type 2. Virology. 1970 Jul;41(3):488–500. doi: 10.1016/0042-6822(70)90170-4. [DOI] [PubMed] [Google Scholar]
  6. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lunney J., Chrambach A., Rodbard D. Factors affecting resolution, band width, number of theoretical plates, and apparent diffusion coefficients in polyacrylamide gel electrophoresis. Anal Biochem. 1971 Mar;40(1):158–173. doi: 10.1016/0003-2697(71)90088-1. [DOI] [PubMed] [Google Scholar]
  8. Maizel J. V., Jr, Summers D. F. Evidence for differences in size and composition of the poliovirus-specific polypeptides in infected HeLa cells. Virology. 1968 Sep;36(1):48–54. doi: 10.1016/0042-6822(68)90115-3. [DOI] [PubMed] [Google Scholar]
  9. Maizel J. V., Jr, White D. O., Scharff M. D. The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. Virology. 1968 Sep;36(1):115–125. doi: 10.1016/0042-6822(68)90121-9. [DOI] [PubMed] [Google Scholar]
  10. Maizel J. V., Jr, White D. O., Scharff M. D. The polypeptides of adenovirus. II. Soluble proteins, cores, top components and the structure of the virion. Virology. 1968 Sep;36(1):126–136. doi: 10.1016/0042-6822(68)90122-0. [DOI] [PubMed] [Google Scholar]
  11. Rodbard D., Kapadia G., Chrambach A. Pore gradient electrophoresis. Anal Biochem. 1971 Mar;40(1):135–157. doi: 10.1016/0003-2697(71)90087-x. [DOI] [PubMed] [Google Scholar]
  12. Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES