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. 1971 Nov;22(5):812–817. doi: 10.1128/am.22.5.812-817.1971

Reversal of the Antimicrobial Activity of Trimethoprim by Thymidine in Commercially Prepared Media

Audrey E Koch 1, James J Burchall 1
PMCID: PMC376424  PMID: 4943586

Abstract

The ability of a potent dihydrofolate reductase inhibitor, trimethoprim, to inhibit the growth of Escherichia coli B in vitro is dependent on the composition of the medium in which the cells are grown. The inhibition observed in minimal broth could be partially reversed by the addition of thymidine, ribonucleosides, amino acids, and vitamins. No reversal occurred in the absence of thymidine. In a number of commercially prepared media, the inhibitory activity of trimethoprim correlated inversely with the amount of thymidine found to be present by microbiological assay. The significance of these findings for the routine testing of new, synthetic antibacterial agents is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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