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. 1971 Dec;22(6):1064–1069. doi: 10.1128/am.22.6.1064-1069.1971

Rapid Detection of Salmonella Microcolonies by Fluorescent Antibody

Berenice M Thomason 1
PMCID: PMC376486  PMID: 4944805

Abstract

A microcolony fluorescent-antibody (FA) procedure for detecting salmonellae was compared to the usual direct FA procedure on 304 environmental, food, and feed samples. The microcolony FA test detected all of the specimens found positive by culture, whereas the direct FA missed 3.1% of them. Both FA tests revealed stained organisms in some of the culturally negative specimens. The microcolony FA test has several advantages over the direct FA test: ease of examining the smears, elimination of the fluorescent background material, and increased sensitivity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHADWICK P., ABBOTT L. SPECIFICITY AND SENSITIVITY OF A MICROCOLONY TECHNIQUE FOR FLUORESCENT ANTIBODY IDENTIFICATION OF PATHOGENIC ESCHERICHIA COLI SEROTYPES. Can J Microbiol. 1964 Dec;10:853–859. doi: 10.1139/m64-111. [DOI] [PubMed] [Google Scholar]
  2. Cherry W. B., Thomason B. M. Fluorescent antibody techniques for Salmonella and other enteric pathogens. Public Health Rep. 1969 Oct;84(10):887–898. [PMC free article] [PubMed] [Google Scholar]
  3. Ellis E. M., Harrington R., Jr A direct fluorescent antibody test for Salmonella. Arch Environ Health. 1969 Dec;19(6):876–881. doi: 10.1080/00039896.1969.10666946. [DOI] [PubMed] [Google Scholar]
  4. Fantasia L. D. Accelerated immunofluorescence procedure for the detection of Salmonella in foods and animal by-products. Appl Microbiol. 1969 Nov;18(5):708–713. doi: 10.1128/am.18.5.708-713.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GEORGALA D. L., BOOTHROYD M. A RAPID IMMUNOFLUORESCENCE TECHNIQUE FOR DETECTING SALMONELLAE IN RAW MEAT. J Hyg (Lond) 1964 Sep;62:319–327. doi: 10.1017/s0022172400040031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Georgala D. L., Boothroyd M., Hayes P. R. Further evaluation of a rapid immunofluorescence technique for detecting salmonellae in meat and poultry. J Appl Bacteriol. 1965 Dec;28(3):421–425. doi: 10.1111/j.1365-2672.1965.tb02172.x. [DOI] [PubMed] [Google Scholar]
  7. Goepfert J. M., Mann M. E., Hicks R. One-day fluorescent-antibody procedure for detecting salmonellae in frozen and dried foods. Appl Microbiol. 1970 Dec;20(6):977–983. doi: 10.1128/am.20.6.977-983.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HAGLUND J. R., AYRES J. C., PATON A. M., KRAFT A. A., QUINN L. Y. DETECTION OF SALMONELLA IN EGGS AND EGG PRODUCTS WITH FLUORESCENT ANTIBODY. Appl Microbiol. 1964 Sep;12:447–450. doi: 10.1128/am.12.5.447-450.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Insalata N. F., Schulte S. J., Berman J. H. Immunofluorescence technique for the detection of salmonellae in various foods. Appl Microbiol. 1967 Sep;15(5):1145–1149. doi: 10.1128/am.15.5.1145-1149.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laramore C. R., Moritz C. W. Fluorescent-antibody technique in detection of salmonellae in animal feed and feed ingredients. Appl Microbiol. 1969 Mar;17(3):352–354. doi: 10.1128/am.17.3.352-354.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Thomason B. M., Wells J. G. Preparation and testing of polyvalent conjugates for fluorescent-antibody detection of salmonellae. Appl Microbiol. 1971 Nov;22(5):876–884. doi: 10.1128/am.22.5.876-884.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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