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. 2013 Jul 22;122(10):1793–1801. doi: 10.1182/blood-2013-06-508952

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© 2013 by The American Society of Hematology

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Decrease in detectable KEL glycoprotein beyond 10 minutes cannot solely be attributed to clearance of KEL2 RBCs with the highest antigen level. (A) Following passive immunization and transfusion as previously described, we calculated clearance rates of DiI-labeled KEL2 RBCs between time points. (B) Changes in detectable KEL glycoprotein levels between time points was calculated by creating a gate that includes the cells from a particular time point and overlaying that gate onto the antigen level of the previous time point to determine the percentage change. (C) Representative bar graphs comparing the data from panels A and B. These data are representative of 5 independent experiments, with 3-5 animals/group/experiment. Error bars represent standard deviation. *Statistical significance, P < .01.