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. 1972 Sep;24(3):430–436. doi: 10.1128/am.24.3.430-436.1972

Fermentation Process for Double-Stranded Ribonucleic Acid, an Interferon Inducer

B D Lago 1,2, J Birnbaum 1,2, A L Demain 1,2,1
PMCID: PMC376536  PMID: 4562479

Abstract

Double-stranded ribonucleic acid (ds-RNA) isolated from Escherichia coli infected with bacteriophage MS2 is a potent interferon inducer. High levels of ds-RNA are formed in nonpermissive cells infected with MU9, an amber coat protein mutant of MS2. This mutant has been used to develop a process for large-scale ds-RNA production. Preparation of quantities of MU9 lysate sufficient for ds-RNA production in fermentors is described. Over 300 μg of ds-RNA/ml can be accumulated after MU9 infection of cultures grown to high density in corn steep liquor medium. This is approximately 300 times the amount of ds-RNA made by MS2 infection of cells grown in tryptone medium. Maximum ds-RNA formation requires only 3 hr. The ds-RNA is stable and remains inside nonaerated cells for at least 17 hr.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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