Abstract
Tissues soaked at low temperature in a trypsin-citrate solution, washed to remove enzymes, and disrupted by rapid mechanical vibrations gave yields of viable cells 1.6 to 5 times those from conventional procedures.
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Selected References
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- BISHOP L. W., SMITH M. K., BEALE A. J. An apparatus for producing trypsinized monkey kidney cell suspensions. Virology. 1960 Feb;10:280–283. doi: 10.1016/0042-6822(60)90048-9. [DOI] [PubMed] [Google Scholar]
- DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MELNICK J. L., RIORDAN J. T. Poliomyelitis viruses in tissue culture. IV. Protein-free nutrient media in stationary and roller tube cultures. Proc Soc Exp Biol Med. 1952 Oct;81(1):208–213. doi: 10.3181/00379727-81-19823. [DOI] [PubMed] [Google Scholar]
- RAPPAPORT C. Trypsinization of monkey-kidney tissue: an automatic method for the preparation of cell suspensions. Bull World Health Organ. 1956;14(1):147–166. [PMC free article] [PubMed] [Google Scholar]
