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. 1972 Sep;24(3):506–507. doi: 10.1128/am.24.3.506-507.1972

Improved Cell Dispersal Method by Low-Temperature Incubation of Tissue in a Trypsin-Citrate Solution

A L van Wezel 1,2, P A van Hemert 1,2, W Parisius 1,2, N B Cucakovich 1,2, H G Macmorine 1,2
PMCID: PMC376550  PMID: 16349935

Abstract

Tissues soaked at low temperature in a trypsin-citrate solution, washed to remove enzymes, and disrupted by rapid mechanical vibrations gave yields of viable cells 1.6 to 5 times those from conventional procedures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BISHOP L. W., SMITH M. K., BEALE A. J. An apparatus for producing trypsinized monkey kidney cell suspensions. Virology. 1960 Feb;10:280–283. doi: 10.1016/0042-6822(60)90048-9. [DOI] [PubMed] [Google Scholar]
  2. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. MELNICK J. L., RIORDAN J. T. Poliomyelitis viruses in tissue culture. IV. Protein-free nutrient media in stationary and roller tube cultures. Proc Soc Exp Biol Med. 1952 Oct;81(1):208–213. doi: 10.3181/00379727-81-19823. [DOI] [PubMed] [Google Scholar]
  4. RAPPAPORT C. Trypsinization of monkey-kidney tissue: an automatic method for the preparation of cell suspensions. Bull World Health Organ. 1956;14(1):147–166. [PMC free article] [PubMed] [Google Scholar]

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