Skip to main content
PMC home page
Applied Microbiology logoLink to Applied Microbiology
. 1970 Feb;19(2):207–213. doi: 10.1128/am.19.2.207-213.1970

Recovery of Anaerobic Microorganisms from Clinical Specimens in Prereduced Media Versus Recovery by Routine Clinical Laboratory Methods 1

M Talmage McMinn 1, James J Crawford 1
PMCID: PMC376653  PMID: 4908525

Abstract

Prereduced anaerobically sterilized culture media, used with rigid adherence to the cultivation techniques described by Moore and his associates, were capable of recovering more than twice the number of anaerobic bacteria from clinical specimens than could be recovered by the conventional use of fluid thioglycolate medium and of blood-agar plates incubated anaerobically with hydrogen generation packets. No loss of clinical isolates was encountered with the more sensitive methods; however many of the isolates recovered only in prereduced media would not grow when placed into thioglycolate medium. A representative anaerobic isolate placed into aerobic transport broth was unable to survive beyond 30 min. Methods employing prereduced media were not difficult to master and were feasible for clinical laboratory use. Evidence implicating the gingival crevice flora as an important possible source of anaerobic bacteria that become involved in systemic infections was considered.

Full text

PDF
207

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brewer J. H., Allgeier D. L. Safe Self-contained Carbon Dioxide-Hydrogen Anaerobic System. Appl Microbiol. 1966 Nov;14(6):985–988. doi: 10.1128/am.14.6.985-988.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Conner H. D., Haberman S., Collings C. K., Winford T. E. Bacteremias following periodontal scaling in patients with healthy appearing gingiva. J Periodontol. 1967 Nov-Dec;38(6):466–472. doi: 10.1902/jop.1967.38.6_part1.466. [DOI] [PubMed] [Google Scholar]
  3. ERNSTENE A. C., McGARVEY C. J., ECKER J. A. The prophylaxis of subacute bacterial endocarditis. Cleve Clin Q. 1951 Jan;18(1):1–5. doi: 10.3949/ccjm.18.1.1. [DOI] [PubMed] [Google Scholar]
  4. HARVEY W. P., CAPONE M. A. Bacterial endocarditis related to cleaning and filling of teeth with particular reference to the inadequacy of present day knowledge and practice of antibiotic prophylaxis for all dental procedures. Am J Cardiol. 1961 Jun;7:793–798. doi: 10.1016/0002-9149(61)90397-6. [DOI] [PubMed] [Google Scholar]
  5. HUNGATE R. E. The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950 Mar;14(1):1–49. doi: 10.1128/br.14.1.1-49.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Khairat O. The non-aerobes of post-extraction bacteremia. J Dent Res. 1966 Jul-Aug;45(4):1191–1197. doi: 10.1177/00220345660450042601. [DOI] [PubMed] [Google Scholar]
  7. McVAY L. V., Jr, SPRUNT D. H. Bacteroides infections. Ann Intern Med. 1952 Jan;36(1):56–76. doi: 10.7326/0003-4819-36-1-56. [DOI] [PubMed] [Google Scholar]
  8. Moore W. E., Cato E. P., Holdeman L. V. Anaerobic bacteria of the gastrointestinal flora and their occurrence in clinical infections. J Infect Dis. 1969 Jun;119(6):641–649. doi: 10.1093/infdis/119.6.641. [DOI] [PubMed] [Google Scholar]
  9. STOKES E. J. Anaerobes in routine diagnostic cultures. Lancet. 1958 Mar 29;1(7022):668–670. doi: 10.1016/s0140-6736(58)91087-0. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES