Skip to main content
Applied Microbiology logoLink to Applied Microbiology
. 1970 Feb;19(2):287–289. doi: 10.1128/am.19.2.287-289.1970

Separation of Treponema pallidum from Tissue Debris Through Continuous-Particle Electrophoresis

John D Schmale 1, Douglas S Kellogg Jr 1, Curtis E Miller 1,1, Patricia Schammel 1,1, James D Thayer 1,2
PMCID: PMC376669  PMID: 4908530

Abstract

Treponema pallidum can only be cultured in living animal tissue, such as rabbit testes. However, the extract of these organisms from the testicular material leaves the T. pallidum contaminated with tissue debris. This paper describes the separation of T. pallidum from the debris by continuous-particle electrophoresis. The importance of equilibration time before electrophoresis is discussed.

Full text

PDF
287

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. HUNTER E. F., DEACON W. E., MEYER P. E. AN IMPROVED FTA TEST FOR SYPHILIS, THE ABSORPTION PROCEDURE (FTA-ABS). Public Health Rep. 1964 May;79:410–412. [PMC free article] [PubMed] [Google Scholar]
  2. PORTNOY J., MAGNUSON H. J. Treponema pallidum complement-fixation (TPCF) test for syphilis. Am J Clin Pathol. 1956 Mar;26(3):313–322. doi: 10.1093/ajcp/26.3_ts.313. [DOI] [PubMed] [Google Scholar]
  3. RATHLEV T., PFAU C. J. PURIFICATION OF THE PATHOGENIC TREPONEMA PALLIDUM BY DENSITY GRADIENT CENTRIFUGATION. Scand J Clin Lab Invest. 1965;17:130–134. doi: 10.1080/00365516509077298. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES