Abstract
A disposable kit was tested as a means of detecting significant bacteriuria by quantitative culture of urine. The total error in 3,563 specimens tested by five investigators was less than 1%. The method was very effective in differentiating significant bacteriuria, i.e., more than 100,000 bacteria per ml of urine from uninfected urine. In specimens from patients with urinary tract abnormalities who had mixed bacterial flora, the absolute numbers obtained with the dip-inoculum method had a 10% variation when compared to results obtained by calibrated loop or dilution pour plate methods. Therefore, the main utility of the kit is for screening and following patients after therapy. A significant delay in time between inoculation of the medium in the kit with the freshly voided urine and incubation of the kit to promote growth did not affect the reliability of the kit as a method of doing quantitative urine cultures to detect bacteriuria.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bailey R. R., Little P. J., Rolleston G. L. Renal damage after acute pyelonephritis. Br Med J. 1969 Mar 1;1(5643):550–551. doi: 10.1136/bmj.1.5643.550. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohen S. N., Kass E. H. A simple method for quantitative urine culture. N Engl J Med. 1967 Jul 27;277(4):176–180. doi: 10.1056/NEJM196707272770403. [DOI] [PubMed] [Google Scholar]
- Dixon J. M., Clarke M. A. Experience with agar-filled plastic spoons for the transport of specimens of urine. Can Med Assoc J. 1968 Oct 19;99(15):741–746. [PMC free article] [PubMed] [Google Scholar]
- KUNIN C. M., ZACHA E., PAQUIN A. J., Jr Urinary-tract infections in schoolchildren. I. Prevalence of bacteriuria and associated urologic findings. N Engl J Med. 1962 Jun 21;266:1287–1296. doi: 10.1056/NEJM196206212662501. [DOI] [PubMed] [Google Scholar]
- Mackey J. P., Sandys G. H. Laboratory diagnosis of infections of the urinary tract in general practice by means of a dip-inoculum transport medium. Br Med J. 1965 Nov 27;2(5473):1286–1288. doi: 10.1136/bmj.2.5473.1286. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TURCK M., GOFFE B. S., PETERSDORF R. G. Bacteriuria of pregnancy. Relation to socioeconomic factors. N Engl J Med. 1962 Apr 26;266:857–860. doi: 10.1056/NEJM196204262661703. [DOI] [PubMed] [Google Scholar]