Abstract
The propagation of various influenza virus strains in primary rhesus monkey kidney (RMK), primary hamster kidney (HK), and the MDCK-USD canine kidney (CK) cell cultures was compared. Virus-infected cultures were examined for cytopathic effect (CPE) and by performing the hemadsorption (HAd) technique. The highest HAd titers were found most often in HK, followed by RMK and CK. However, the CK cell line provided the best substrate for detecting CPE. Although influenza B strains tended to grow to higher titers, these strains produced less CPE than did the A strains.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- GREEN I. J. Serial propagation of influenza B (Lee) virus in a transmissible line of canine kidney cells. Science. 1962 Oct 5;138(3536):42–43. doi: 10.1126/science.138.3536.42. [DOI] [PubMed] [Google Scholar]
- GROSSBERG S. E. Cytocidal assay and propagation of human influenza viruses in cultured primary hamster kidney cells. Proc Soc Exp Biol Med. 1963 Jul;113:546–553. doi: 10.3181/00379727-113-28422. [DOI] [PubMed] [Google Scholar]
- Gaush C. R., Smith T. F. Replication and plaque assay of influenza virus in an established line of canine kidney cells. Appl Microbiol. 1968 Apr;16(4):588–594. doi: 10.1128/am.16.4.588-594.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hatano M., Morita O. Multiplication and plaque assay of influenza viruses in a continuous cell line (G2) of human origin. Arch Gesamte Virusforsch. 1967;20(3):305–313. doi: 10.1007/BF01241950. [DOI] [PubMed] [Google Scholar]
- KILBOURNE E. D., SUGIURA A., WONG S. C. SERIAL MULTIPLICATION OF AN INFLUENZA VIRUS (NWS) IN CERTAIN HUMAN DIPLOID CELL STRAINS. Proc Soc Exp Biol Med. 1964 May;116:225–228. doi: 10.3181/00379727-116-29209. [DOI] [PubMed] [Google Scholar]
- LEHMANN-GRUBE F. INFLUENZA VIRUSES IN CELL CULTURES. I. PREPARATION AND USE OF FETAL PIG LUNG CELLS FOR QUANTAL ASSAY. Arch Gesamte Virusforsch. 1963 Oct 7;14:1–14. [PubMed] [Google Scholar]
- MOGABGAB W. J., GREEN I. J., DIERKHISING O. C., PHILLIPS I. A. Isolation and cytopathogenic effect of influenza B viruses in monkey kidney cultures. Proc Soc Exp Biol Med. 1955 Aug;89(4):654–659. doi: 10.3181/00379727-89-21907. [DOI] [PubMed] [Google Scholar]
- SHELOKOV A., VOGEL J. E., CHI L. Hemadsorption (adsorption-hemagglutination) test for viral agents in tissue culture with special reference to influenza. Proc Soc Exp Biol Med. 1958 Apr;97(4):802–809. doi: 10.3181/00379727-97-23884. [DOI] [PubMed] [Google Scholar]