Abstract
A method was tested for protecting a Streptococcus lactis strain, ML3, used as a starter in the manufacture of Cheddar cheese, against the lytic activity of its homologous phage, ml3. At a concentration of 10−2m, a naturally occurring polyamine, spermine, in the form of its hydrochloride, protected ML3 against lysis-from-without and lysozyme activity and against lysis by the phage when added at the time of infection or up to 21 min after infection. It was found that the latter protective effect could be accounted for in terms of the spermine preventing the formation of mature particles rather than preventing the escape of viable phage. Single colonies selected from a culture of ML3 cells that had been previously infected with phage ml3, in the presence of spermine, were all found to have acquired resistance to phage ml3. They retained this resistance during a 3-month period of daily subculture in broth and, in the absence of spermine, could not be induced to liberate phage or phage components either by the techniques normally used for inducing lysogens or by artificial disruption of the cells. It is concluded that when spermine is added to ML3 cells before a certain critical stage of the phage infection cycle, the process of phage synthesis is irreversibly halted and the cells retain the infecting phage as a defective prophage that confers on the cells immunity to infection by the homologous phage. Phage-resistant cultures did not inherit reduced starter activity in association with their acquired resistance characteristic.
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Selected References
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- COHEN S. S., LICHTENSTEIN J. Polyamines and ribosome structure. J Biol Chem. 1960 Jul;235:2112–2116. [PubMed] [Google Scholar]
- Ezekiel D. H., Brockman H. Effect of spermidine treatment on amino acid availability in amino acid-starved Escherichia coli. J Mol Biol. 1968 Feb 14;31(3):541–552. doi: 10.1016/0022-2836(68)90426-9. [DOI] [PubMed] [Google Scholar]
- Friedman M. E., Bachrach U. Inhibition of protein synthesis by spermine in growing cells of Staphylococcus aureus. J Bacteriol. 1966 Jul;92(1):49–55. doi: 10.1128/jb.92.1.49-55.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GROSSOWICZ N., ARIEL M. Mechanism of protection of cells by spermine against lysozyme-induced lysis. J Bacteriol. 1963 Feb;85:293–300. doi: 10.1128/jb.85.2.293-300.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Groman N. B., Suzuki G. Effect of spermine on lysis and reproduction by bacteriophages phi-X174, lambda, and f2. J Bacteriol. 1966 Dec;92(6):1735–1740. doi: 10.1128/jb.92.6.1735-1740.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HERBST E. J., SNELL E. E. Putrescine and related compounds as growth factors for Hemophilus parainfluenzae 7991. J Biol Chem. 1949 Nov;181(1):47–54. [PubMed] [Google Scholar]
- Imaeda T., Rieber M. Mitomycin C-induced phage-like particles in a mutant of Mycobacterium tuberculosis BCG. J Bacteriol. 1968 Aug;96(2):557–559. doi: 10.1128/jb.96.2.557-559.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAGER J., BENEDICT M., ARTMAN M. A common site of action of polyamines and streptomycin. Biochim Biophys Acta. 1962 Jul 30;62:202–204. doi: 10.1016/0006-3002(62)90519-x. [DOI] [PubMed] [Google Scholar]
- MAGER J. The stabilizing effect of spermine and related polyamines and bacterial protoplasts. Biochim Biophys Acta. 1959 Dec;36:529–531. doi: 10.1016/0006-3002(59)90195-7. [DOI] [PubMed] [Google Scholar]
- ROZANSKY R., BACHRACH U., GROSSOWICZ N. Studies on the antibacterial action of spermine. J Gen Microbiol. 1954 Feb;10(1):11–16. doi: 10.1099/00221287-10-1-11. [DOI] [PubMed] [Google Scholar]
- Tabor C. W. STABILIZATION OF PROTOPLASTS AND SPHEROPLASTS BY SPERMINE AND OTHER POLYAMINES. J Bacteriol. 1962 May;83(5):1101–1111. doi: 10.1128/jb.83.5.1101-1111.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]