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. 1970 May;19(5):785–790. doi: 10.1128/am.19.5.785-790.1970

Method for Extracting Viral Hemagglutination-Inhibiting Antibodies from the Nonspecific Inhibitors of Serum

William A Altemeier III 1,1, Francis K Mundon 1, Franklin H Top Jr 1, Philip K Russell 1
PMCID: PMC376789  PMID: 5463576

Abstract

Various methods are used to remove nonspecific inhibitors from sera before titering viral hemagglutination-inhibiting antibodies. These methods have several undesirable features; some are tedious and time-consuming, some remove antibody along with nonspecific inhibitors, and different techniques are usually required to remove the nonspecific inhibitors for different viruses. This communication describes a single method that uses diethylaminoethyl-Sephadex to extract the immunoglobulin G antibodies for several viruses from nonspecific inhibitors. The procedure is fast, simple to perform, and removed the nonspecific inhibitors for influenza, Western equine encephalitis, dengue-2, and rubella viruses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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