Abstract
A new method of determining bacterial spoilage in fresh beef is presented. The technique is based upon the fact that as beef undergoes refrigerator spoilage, there is a gradual increase in the production of alkaline substances by the spoilage flora. The level of these substances was measured by titrating meat homogenates to a pH 5.00 end point, employing 0.02 n HCl and an autotitrator. When 23 samples of ground beef from retail stores were tested, an average of 1.32 ml of acid was required for titration of 1 g of fresh beef to pH 5.00, whereas 2.58 ml was required for the same meat at the onset of spoilage. Preliminary data indicate that beef which requires more than 2 ml of 0.02 n HCl/g to lower its pH to 5.00 under the conditions of the test is in some state of incipient spoilage. The statistical correlation between titration values, log bacterial numbers, and extract-release volume was high (P < 0.001). The technique is simple to execute and is highly reproducible, and duplicate samples can be run within 15 min.
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Selected References
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- JAY J. M. RELATIONSHIP BETWEEN WATER-HOLDING CAPACITY OF MEATS AND MICROBIAL QUALITY. Appl Microbiol. 1965 Jan;13:120–121. doi: 10.1128/am.13.1.120-121.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jay J. M. Response of the extract-release volume and water-holding capacity phenomena to microbiologically spoiled beef and aged beef. Appl Microbiol. 1966 Jul;14(4):492–496. doi: 10.1128/am.14.4.492-496.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shelef L. A., Jay J. M. Relationship between amino sugars and meat microbial quality. Appl Microbiol. 1969 Jun;17(6):931–932. doi: 10.1128/am.17.6.931-932.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]