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. 1970 Jun;19(6):932–936. doi: 10.1128/am.19.6.932-936.1970

Studies of Laryngotracheitis Virus in Avian Tissue Cultures

III. Enhancement of Infectivity by Diethylaminoethyl-Dextran

C R Rossi 1,1, A M Watrach 1
PMCID: PMC376827  PMID: 5466048

Abstract

Diethylaminoethyl-dextran (DEAE-D) enhanced the infectivity of laryngotracheitis virus (LTV) for chicken kidney (CK) cells when cultures were treated before inoculation with virus and when DEAE-D was present in the inoculum. Infectivity was not increased when cultures were treated after virus had adsorbed to cells; since infection was not synchronized, most of the virus had probably already penetrated the plasma membrane by the time DEAE-D was added. Maximal enhancement occurred when DEAE-D was present in the inoculum. Enhancement of a lesser degree occurred when virus and DEAE-D were mixed, diluted, and inoculated onto cultures. Adsorption of LTV at 37 C as compared to that at 5 C usually yields about a threefold greater number of plaques after a 2-hr adsorption period. However, when DEAE-D was incorporated in the inoculum, greater enhancement occurred at 5 C than at 37 C, and the number of plaques produced at both adsorption temperatures was about equal. Results are compatible with the hypothesis that increased adsorption is a factor in enhancement of infectivity of LTV by DEAE-D.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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