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. 1970 Jun;19(6):973–979. doi: 10.1128/am.19.6.973-979.1970

Volutin Granules in Zoogloea ramigera

Frank A Roinestad 1, Irving Yall 1
PMCID: PMC376836  PMID: 4195479

Abstract

Zoogloea ramigera, a gram-negative bacterium found in activated sludge, formed volutin granules when excess orthophosphate was added to a phosphate-starved culture. These volutin granules were stainable by hydrogen sulfide after lead acetate treatment and extractable by N-perchloric acid but were not adsorbed by activated charcoal. They appeared to consist of inorganic polyphosphate. Optimum granule formation in the arginine broth required 10 g of glucose, 3 mg of phosphate, and 1 to 20 mg of magnesium per liter of medium. At an Mg2+ concentration of 1 mg/liter, very large granules appeared which often appeared to fill the cell. An excess of glucose, orthophosphate, or magnesium reduced granule formation. In the absence of sulfate, moderate granulation occurred in arginine broth before the addition of excess orthophosphate; granulation did not increase after the addition of phosphate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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