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. 1970 Jun;19(6):997–1000. doi: 10.1128/am.19.6.997-1000.1970

Application of Freeze-Etching Method to the Study of Reovirus-Infected LLC-MK2 Cells

K R Rozee 1, K B Easterbrook 1
PMCID: PMC376839  PMID: 4195480

Abstract

A continuous cell line of rhesus monkey kidney cells, LLC-MK2, was infected with reovirus type 1 (Lang). The cells were freeze-etched as a method for observing the structural details of the reovirus-induced cytoplasmic inclusion. Information on maturation may be obtained by preparing infected cells for electron microscopy by freeze-etching.

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Selected References

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  1. Bächi T., Gerhard W., Lindenmann J., Mühlethaler K. Morphogenesis of influenza A virus in Ehrlich ascites tumor cells as revealed by thin-sectioning and freeze-etching. J Virol. 1969 Nov;4(5):769–776. doi: 10.1128/jvi.4.5.769-776.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. STEERE R. L. Electron microscopy of structural detail in frozen biological specimens. J Biophys Biochem Cytol. 1957 Jan 25;3(1):45–60. doi: 10.1083/jcb.3.1.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. TOURNIER P., PLISSIER M. [The intracellular development of the reovirus studied with the electron microscope]. Presse Med. 1960 Apr 9;68:683–688. [PubMed] [Google Scholar]

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