Xylitol production from wheat straw hemicellulosic hydrolysate: hydrolysate detoxification and carbon source used for inoculum preparation

Abstract

Wheat straw hemicellulosic hydrolysate was used for xylitol bioproduction. The use of a xylose-containing medium to grow the inoculum did not favor the production of xylitol in the hydrolysate, which was submitted to a previous detoxification treatment with 2.5% activated charcoal for optimized removal of inhibitory compounds.

Xylitol is a sweetener with outstanding anticariogenic properties. It is suitable for diabetics, prevents osteoporosis, can replace antibiotics in the treatment of otitis and is capable of intervening in the initial steps of infections in patients with cystic fibrosis (7). Its production by biotechnological means is expected to be cheaper than the traditional chemical process because it does not requires pure xylose and is carried out under mild temperature and pressure conditions (7).

In the present study, the carbon source present in the medium used to grow the cells during the preparation of the inoculum was either glucose or a mixture of glucose and xylose. This last sugar was added to the medium used to grow the inoculum in order to induce the expression of enzymes necessary for xylose metabolism, like xylose reductase and xylitol dehydrogenase (16). It was expected that the growth of the cells in an inducing medium would speed up the xylitol bioproduction in the hydrolysate-based medium, since the xylose-to-xylitol bioconversion is influenced by the type and ratio of sugars present in fermentation media (18).

The presence of inhibitory compounds in the hemicellulosic hydrolysate, originated during the hydrolysis of the raw material, negatively influences the xylose-to-xylitol bioconversion. Therefore, the removal of inhibitors (organic acids, sugar degradation compounds and lignin derivatives) from the hydrolysates prior to the fermentation generally leads to improved bioconversion rates and yields (7). A number of methods can be used for hydrolysate detoxification, including pH adjustment (10), adsorption into ion exchange resins (17) and adsorption into active charcoal, which presents a high efficiency associated to a low-cost (11). In the present study, two levels of active charcoal were tested to detoxify the wheat straw hydrolysate prior to xylitol bioproduction. It was expected that a higher amount of active charcoal (adsorbing matrix) would favor the xylitol bioproduction due to a higher elimination of undesirable inhibiting compounds.

The materials and methods were as follows: The wheat straw was hydrolyzed with dilute sulfuric acid (4) and concentrated threefold at 70°C under vacuum. The pH of the concentrated hydrolysate was then raised to 7.0 with CaO and reduced to 5.5 with H3PO4 (1). The hydrolysate thus obtained was submitted to adsorption into active charcoal at 200 rpm and 30°C for 1 h. The ratio between active charcoal mass and hydrolysate volume was 1:10 (10% active charcoal adsorption - HD₁) or 1:40 (2.5% active charcoal adsorption - HD₂). After removal of the precipitate by vacuum filtration, the hydrolysates were sterilized at 111°C for 15 min. The yeast Candida guilliermondii FTI 20037 (2) was used to promote the xylose-to-xylitol bioconversions. A loopful of cells was transferred to 125-mL Erlenmeyer flasks containing 50 mL of medium consisting of (g/ L) 2.0 ammonium sulfate, 0.1 calcium chloride and 20.0 rice bran. Either one or two carbon sources were used to grow the cells, namely 30.0 xylose and 7.0 glucose (IP1) or 37.0 glucose (IP2). The cells were then incubated at 30°C and 200 rpm for 24 h, collected by a 15-min centrifugation at 2000 g and re-suspended in sterile distilled water. The fermentations (triplicates) were carried out in 125-mL Erlenmeyer flasks containing 50 mL of medium and 0.5 g/L initial cell concentration, at 30°C and 200 rpm for 72 h. The medium was composed by the detoxified hydrolysates supplemented with (g/L) 1.0 ammonium sulfate and 5.0 rice bran extract, and had its pH adjusted to 6.0 prior to inoculation (4). Sugars, xylitol, acetic acid, furfural and hydroxymethylfurfural (HMF) concentrations were determined by HPLC (17). Lignin derivatives were determined by UV spectroscopy (4). Cell concentrations were determined by VIS spectroscopy and correlated with the cell dry-weight (4).

Fig. 1-A shows the profiles of sugars and acetic acid consumptions, of xylitol production and of cell growth during the bioconversion carried out with cells grown in a medium elaborated with xylose + glucose (IP1) and hydrolysate detoxified with 10% active charcoal (HD1). As can be seen, glucose was rapidly consumed within the first 24 h of fermentation. At the same period, the yeast consumed 31% of the available xylose, thus indicating that a simultaneous consumption of xylose and glucose may have occurred in the assay. Such a simultaneous consumption of xylose and glucose was also previously observed by Silva and Felipe (16). Arabinose and acetic acid were also assimilated by the yeast during the fermentation. It is known that the presence of arabinose induces the enzyme xylose reductase and stimulates the yield and productivity of the xylose-to-xylitol bioconversion (18). A low consumption of acetic acid has previously been reported to improve the microbial growth (5). In the present study, a high cell concentration (7.2 g/ L) was observed at the end of the assay. During the first 24 h, the cells exhibited a pronounced growth, possibly due to the availability of the six-carbon sugar glucose. Afterwards, between 24 and 48 h, a “stationary” phase with no cellular growth and pronounced xylitol production was observed. By the end of the fermentation, a xylitol production of 24.2 g/L was observed, resulting in a productivity of 0.34 g/L h and in a bioconversion yield of 0.48 g/g (efficiency of 52.3%).

Figure 1.

Profiles of glucose (closed square), xylose (closed circle), arabinose (closed lozenge) and acetic acid (open circle) consumptions as well as of xylitol production (closed triangle) and of cell growth (X). Assays: A) HD₁ + IP₁, B) HD₂ + IP₁, C) HD₂ + IP₂. Average values of triplicates with standard deviations (error bars).

The presence of glucose in the fermentation medium can influence the xylose-to-xylitol bioconversion. For instance, when cultivated in a wood hemicellulosic hydrolysate, the yeast Candida parapsilosis exhibited a faster and more efficient bioconversion in the presence of glucose (glucose:xylose ratio of 1:2.5) than in the absence of this sugar (14). In other example (8), a two-substrate fermentation optimized for xylitol production with the yeast Candida tropicalis (32 g/L glucose and 100 g/L xylose) led to a bioconversion yield of 0.81 g/g and to a productivity of 5.06 g/L h. Such values were 1.4 and 1.85 times higher, respectively, than those obtained in the absence of glucose. For the yeast Candida guilliermondii FTI 20037, a ratio between glucose and xylose of 1:5 was previously demonstrated to improve the bioconversion yield (0.59 g/g) and productivity (0.53 g/L h) in a sugarcane bagasse-based fermentation medium (16). For the yeast C. tropicalis, glucose concentrations of up to 20 g/L did not hinder the bioconversion in a xylose-based medium (18). In the present study, the glucose:xylose ratio was 1:2.8 and resulted in bioconversion yield of 0.48 g/g and productivity of 0.34 g/L h.

As can be seen in Fig. 1-B, the use of a lower amount of active charcoal (2.5%) to detoxify the hydrolysate resulted in profiles of xylose consumption, xylitol production and cell growth similar to those observed when the hydrolysate was detoxified with 10% active charcoal (Fig. 1-A). In fact, the bioconversion parameters were slightly increased (production of 28.6 g/L, productivity of 0.40 g/L h and yield of 0.54 g/g), suggesting that only 2.5% active charcoal is sufficient to promote a good detoxification of the wheat straw hydrolysate prior to fermentation. A similar behavior was also observed in rice straw hydrolysate, when the best xylose-to-xylitol bioconversion yield (0.72 g/g) and productivity (0.61 g/L h) were achieved by promoting the detoxification with only 2.5% active charcoal (11).

Fig. 1-C shows the profiles of sugars and acetic acid consumption, xylitol production and cell growth during the bioconversion whose inoculum was grown in a medium with glucose (37 g/L) as the sole carbon source. In this experiment, only 2.5% active charcoal was used to detoxify the hydrolysate. In this assay, the bioconversion parameters were slightly better than those observed when both glucose (7 g/L) and xylose (30 g/L) were used as carbon sources in the medium used to grow the inoculum (Figure 1-B). A xylitol production of 30.5 g/L was achieved after 72 h of fermentation, resulting in productivity of 0.42 g/L h and bioconversion yield of 0.59 g/g. Such findings suggest that the cheaper sugar glucose can be used as the sole carbon source to grow the C. guilliermondii FTI 20037 cells for a subsequent xylose-to-xylitol bioconversion in wheat straw hemicellulosic hydrolysate. Moreover, they confirm the validity of using glucose as the sole carbon source for inoculum preparation in experiments aimed at xylitol bioproduction (9,12-13,15).

As can be seen in Table 1, the best results achieved in the present study (xylitol production of 30.5 g/L, productivity of 0.42 g/L h and yield of 0.59 g/g) were lower than those obtained in sugarcane bagasse (1) and rice straw (11) hydrolysates. At the same time, they were higher than those observed in eucalyptus hydrolysates (3,17) and in an immobilized cell system (6). It is difficult to compare the results obtained by different researchers since many variables influence the xylose-to-xylitol bioconversion in hemicellulosic hydrolysates. In spite of this, the interesting parameters of bioconversion attained in the rice straw hydrolysate may certainly be related with the high xylose concentration used in the experiment. While a considerably high bioconversion yield could be achieved in the sugarcane bagasse hydrolysate using a relatively low initial cell concentration (0.5 g/L), the poor bioconversions observed in the eucalyptus hydrolysate were attributed to a very high concentration of inhibiting hydrolysis by-products.

Table 1.

Data regarding xylitol bioproduction with the yeast C. guilliermondii FTI 20037 in hydrolysates from different raw materials.

Raw material	MD	CS	Xo (g/L)	So (g/L)	P (g/L)	QP(g/Lh)	YP/S(g/g)	Reference
Wheat straw	pH alteration with 2.5% active charcoal	Glucose (37.0 g/L)	0.5	51,7	30.5	0.42	0.59	this work
Sugarcane bagasse	pH alteration with 2.4% active charcoal	Xylose (30.0 g/L)	0.5	-	29.6	0.47	0.79	(1)
Sugarcane bagasse	pH alteration with 2.4% active charcoal	Xylose (30.0 g/L)	1.6*	43.4	21.0	0.44	0.54	(6)
Eucalyptus wood	pH alteration with 10.0% active charcoal	Xylose (30.0 g/L) and Glucose (7.0 g/L)	3.0	60.0	10.0	0.10	0.20	(3)
Eucalyptus wood	pH alteration with 5.0% active charcoal	Xylose (30.0 g/L) and Glucose (2.0 g/L)	3.0	56.5	11.1	0.09	0.26	(17)
Rice straw	pH alteration with 2.5% active charcoal	Xylose (20.0 g/L)	3.0	90.0	59.0	0.61	0.72	(11)

MD: Method used for hydrolysate detoxification; CS: Carbon source used for inoculum preparation; Xo: Initial cell concentration; So: Initial xylose concentration; P: Xylitol production; Qp: Xylitol productivity; Yp/_S: Xylose-to-xylitol conversion yield; -: data not presented.

As a whole, the present study points to the fact that the wheat straw is a potential raw material to be used as a source of xylose for xylitol bioproduction. The toxic compounds present in the hydrolysate can be efficiently removed by a simple detoxification strategy based on pH alteration and active charcoal adsorption. Moreover, glucose can be used as the sole carbon source to grow the inoculum of C. guilliermondii FTI 20037 cells in order to provide good xylose-to-xylitol bioconversion rates and yields in the detoxified hydrolysate.

RESUMO

Produção de xilitol em hidrolisado hemicelulósico de palha de trigo: destoxificação do hidrolisado e fonte de carbono utilizada para o preparo do inóculo

Hidrolisado hemicelulósico de palha de trigo foi utilizado para a bioprodução de xilitol. O uso de meio contendo xilose para crescer o inóculo não favoreceu a produção de xilitol no hidrolisado, que foi submetido a um tratamento prévio de destoxificação com 2.5% de carvão ativo para remoção otimizada de compostos inibitórios.

Palavras-chave:Hidrolisado hemicelulósico de palha de trigo; Destoxificação do hidrolisado; Preparo do inóculo; Candida guilliermondii; Produção de xilitol.