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. 1970 Jun;19(6):1001–1004. doi: 10.1128/am.19.6.1001-1004.1970

Cytochemical Electron Microscopic Localization of Esterase Activity in Lactobacillus casei

J P Brown 1
PMCID: PMC376840  PMID: 4195477

Abstract

Enzymes capable of hydrolyzing thiolacetic acid in two strains of Lactobacillus casei were localized by the formation of electron-opaque PbS deposits in vivo in the presence of Pb(NO3)2. By electron microscopy, the deposition of PbS appeared primarily at the plasma membrane and at the outer surface of the cell wall.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARRNETT R. J., PALADE G. E. Enzymatic activity in the M band. J Biophys Biochem Cytol. 1959 Oct;6:163–170. doi: 10.1083/jcb.6.2.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baillie A., Thomson R. O., Batty I., Walker P. D. Some preliminary observations on the location of esterases in Bacillus cereus. J Appl Bacteriol. 1967 Aug;30(2):312–316. doi: 10.1111/j.1365-2672.1967.tb00302.x. [DOI] [PubMed] [Google Scholar]
  3. Brown J. P., Edwards M. R., VanDemark P. J. Ultrastructure and sites of tetranito-blue tetrazolium reduction of Lactobacillus casei. Can J Microbiol. 1968 Aug;14(8):823–827. doi: 10.1139/m68-140. [DOI] [PubMed] [Google Scholar]
  4. Brown J. P., VanDemark P. J. Respiration of Lactobacillus casei. Can J Microbiol. 1968 Aug;14(8):829–835. doi: 10.1139/m68-141. [DOI] [PubMed] [Google Scholar]
  5. Harold F. M. Inorganic polyphosphates in biology: structure, metabolism, and function. Bacteriol Rev. 1966 Dec;30(4):772–794. doi: 10.1128/br.30.4.772-794.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KELLENBERGER E., RYTER A., SECHAUD J. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J Biophys Biochem Cytol. 1958 Nov 25;4(6):671–678. doi: 10.1083/jcb.4.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Morichi T., Sharpe M. E., Reiter B. Esterases and other soluble proteins of some lactic acid bacteria. J Gen Microbiol. 1968 Oct;53(3):405–414. doi: 10.1099/00221287-53-3-405. [DOI] [PubMed] [Google Scholar]
  8. Sargent M. G., Ghosh B. K., Lampen J. O. Localization of cell-bound penicillinase in Bacillus licheniformis. J Bacteriol. 1968 Oct;96(4):1329–1338. doi: 10.1128/jb.96.4.1329-1338.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tice L. W. Lead-adenosine triphosphate complexes in adenosine triphosphatase histochemistry. J Histochem Cytochem. 1969 Feb;17(2):85–94. doi: 10.1177/17.2.85. [DOI] [PubMed] [Google Scholar]

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