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. 1970 Jul;20(1):64–68. doi: 10.1128/am.20.1.64-68.1970

Recovery of Rauscher Leukemia Virus from Large Volumes of Seeded Cow's Milk and from Infected Murine Spleens

Edward P Larkin 1, Ray M Dutcher 1
PMCID: PMC376867  PMID: 5456940

Abstract

Rauscher murine leukemia virus was used as an indicator agent to develop a methodology for the extraction and concentration of a theoretical leukemia virus from bovine milk and tissues. The indicator virus was seeded into cow's milk or was recovered from infected murine spleens. The tissue homogenates and the defatted milk were processed in a B-XVI rotor of a Spinco L-4 ultracentrifuge at a flow rate of 3 liters/hr. The efficiency of Rauscher virus recovery was greatest when the rotor was used without a gradient. A loss of between 0.6 and 0.7 log of total infectious virus, as determined by the spleen assay method, resulted when the seeded milk and murine spleens were processed. The procedures developed are presently being used in transmission experiments in an attempt to induce leukemia in the bovine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson N. G., Barringer H. P., Amburgey J. W., Jr, Cline G. B., Nunley C. E., Berman A. S. Continuous-flow centrifugation combined with isopycnic banding: rotors B-8 and B-IX. Natl Cancer Inst Monogr. 1966 Jun;21:199–216. [PubMed] [Google Scholar]
  2. CHIRIGOS M. A. STUDIES WITH THE MURINE LEUKEMOGENIC RAUSCHER VIRUS. 3. AN IN VIVO ASSAY FOR ANTI-VIRAL AGENTS. Cancer Res. 1964 Jul;24:1035–1041. [PubMed] [Google Scholar]
  3. Cornefert-Jensen F., Hare W. C., Stock N. D. Studies on bovine lymphosarcoma: formation of syncytia and detection of virus particles in mixed-cell cultures. Int J Cancer. 1969 Jul 15;4(4):507–519. doi: 10.1002/ijc.2910040416. [DOI] [PubMed] [Google Scholar]
  4. DMOCHOWSKI L. VIRUSES AND CANCER IN ANIMALS AND MAN. South Med J. 1964 Nov;57:1267–1272. doi: 10.1097/00007611-196411000-00003. [DOI] [PubMed] [Google Scholar]
  5. Dmochowski L. Electron microscopic observations of leukemia in animals and in man. Cancer Res. 1965 Oct;25(9):1654–1671. [PubMed] [Google Scholar]
  6. Dmochowski L. Viruses and their relationship to animal and human tumors. Tex Rep Biol Med. 1965 Fall;23(3):539–561. [PubMed] [Google Scholar]
  7. Dutcher R. M., Larkin E. P., Tumilowicz J. J., Nazerian K., Eusebio C. P., Stock N. D., Guest G. B., Marshak R. R. Evidence in support of a virus etiology for bovine leukemia. Cancer. 1967 May;20(5):851–856. doi: 10.1002/1097-0142(1967)20:5<851::aid-cncr2820200544>3.0.co;2-o. [DOI] [PubMed] [Google Scholar]
  8. FRIEND C. Cell-free transmission in adult Swiss mice of a disease having the character of a leukemia. J Exp Med. 1957 Apr 1;105(4):307–318. doi: 10.1084/jem.105.4.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GROSS L. Transmission of mouse leukemia virus through milk of virus-injected C3H female mice. Proc Soc Exp Biol Med. 1962 Apr;109:830–836. doi: 10.3181/00379727-109-27350. [DOI] [PubMed] [Google Scholar]
  10. Gerin J. L., Richter W. R., Fenters J. D., Holper J. C. Use of zonal ultracentrifuge systems for biophysical studies of rhinoviruses. J Virol. 1968 Sep;2(9):937–943. doi: 10.1128/jvi.2.9.937-943.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Larkin E. P., Dutcher R. M., Charney J. Bovine leukemia: the use of citrate for the preparation of milk samples for examination by electron microscopy. Am J Vet Res. 1966 Mar;27(117):594–596. [PubMed] [Google Scholar]
  12. MOLONEY J. B. Biological studies on a lymphoid-leukemia virus extracted from sarcoma 37. I. Origin and introductory investigations. J Natl Cancer Inst. 1960 Apr;24:933–951. [PubMed] [Google Scholar]
  13. Malmquist W. A., Van der Maaten M. J., Boothe A. D. Isolation, immunodiffusion, immunofluorescence, and electron microscopy of a syncytial virus of lymphosarcomatous and apparently normal cattle. Cancer Res. 1969 Jan;29(1):188–200. [PubMed] [Google Scholar]
  14. Miller J. M., Miller L. D., Olson C., Gillette K. G. Virus-like particles in phytohemagglutinin-stimulated lymphocyte cultures with reference to bovine lymphosarcoma. J Natl Cancer Inst. 1969 Dec;43(6):1297–1305. [PubMed] [Google Scholar]
  15. Mirand E. A., Buffett R. F., Grace J. T., Jr Mode of transmission of Friend virus disease. Proc Soc Exp Biol Med. 1966 Mar;121(3):970–976. doi: 10.3181/00379727-121-30939. [DOI] [PubMed] [Google Scholar]
  16. O'CONNOR T. E., RAUSCHER F. J., ZEIGEL R. F. DENSITY GRADIENT CENTRIFUGATION OF A MURINE LEUKEMIA VIRUS. Science. 1964 May 29;144(3622):1144–1147. doi: 10.1126/science.144.3622.1144. [DOI] [PubMed] [Google Scholar]
  17. RAUSCHER F. J. A virus-induced disease of mice characterized by erythrocytopoiesis and lymphoid leukemia. J Natl Cancer Inst. 1962 Sep;29:515–543. [PubMed] [Google Scholar]
  18. Rickard C. G., Post J. E., Noronha F., Barr L. M. A transmissible virus-induced lymphocytic leukemia of the cat. J Natl Cancer Inst. 1969 Jun;42(6):987–1014. [PubMed] [Google Scholar]
  19. Theilen G. H., Dungworth D. L., Harrold J. B., Straub O. C. Bovine lymphosarcoma transmission studies. Am J Vet Res. 1967 Mar;28(123):373–386. [PubMed] [Google Scholar]
  20. Toplin I. Purification of the Moloney and Rauscher murine leukemia viruses by use of zonal ultracentrifuge systems. Appl Microbiol. 1967 May;15(3):582–589. doi: 10.1128/am.15.3.582-589.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tumilowicz J. J., Shirahama S. Aberrant characteristics and virus-like particles of bovine mammary cell cultures. Am J Vet Res. 1969 Jan;30(1):51–59. [PubMed] [Google Scholar]

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