Skip to main content
Applied Microbiology logoLink to Applied Microbiology
. 1970 Jul;20(1):75–77. doi: 10.1128/am.20.1.75-77.1970

Simplified Procedure for Preparation of Specific Antibodies to Gamma Globulins

Richard G Olsen 1, James R McCammon 1, David S Yohn 1
PMCID: PMC376870  PMID: 4989671

Abstract

Antibodies were prepared to rhesus monkey, rabbit, dog, and hamster gamma globulins which had been purified by agar-block electrophoresis. Immunoelectrophoretic analysis demonstrated that these antisera were specific for gamma globulin components in whole serum. The use of these antisera as secondary serological reagents was examined.

Full text

PDF
75

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bloch K. J., Morse H. C., 3rd, Austen K. F. Biologic properties of rat antibodies. I. Antigen-binding by four classes of anti-DNP antibodies. J Immunol. 1968 Oct;101(4):650–657. [PubMed] [Google Scholar]
  2. Exum E. D., Bowser R. T. Purification of rat gamma globulin and the production of a specific antirat gamma globulin serum. Proc Soc Exp Biol Med. 1970 Jan;133(1):43–48. doi: 10.3181/00379727-133-34403. [DOI] [PubMed] [Google Scholar]
  3. FAGRAEUS A., ESPMARK A. Use of a 'mixed haemadsorption' method in virus-infected tissue cultures. Nature. 1961 Apr 22;190:370–371. doi: 10.1038/190370a0. [DOI] [PubMed] [Google Scholar]
  4. Holmes E. C., Morton D. L. Detection of antibodies against the mammary tumor virus with the antiglobulin consumption test. J Natl Cancer Inst. 1969 May;42(5):733–738. [PubMed] [Google Scholar]
  5. Reif A. E. Batch preparation of rabbit gammaG globulin with deae-cellulose. Immunochemistry. 1969 Sep;6(5):723–731. doi: 10.1016/0019-2791(67)90136-x. [DOI] [PubMed] [Google Scholar]
  6. Sparks F. C., Ting C. C., Hammond W. G., Herberman R. B. An isotopic antiglobulin technique for measuring antibodies to cell-surface antigens. J Immunol. 1969 Apr;102(4):842–847. [PubMed] [Google Scholar]
  7. Yohn D. S., Funk C. A., Grace J. T., Jr Sex-related resistance in hamsters to adenovirus-12 oncogenesis. II. Influence of virus dose. J Virol. 1967 Dec;1(6):1186–1192. doi: 10.1128/jvi.1.6.1186-1192.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Yohn D. S., Marmol F. R., Olsen R. G. Growth kinetics of Yaba tumor poxvirus after in vitro adaptation to cercopithecus kidney cells. J Virol. 1970 Feb;5(2):205–211. doi: 10.1128/jvi.5.2.205-211.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES